International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article    |    Open Access
International Journal of Innovative Approaches in Agricultural Research 2018, Vol. 2(4) 391-407

The Influence of Protein Content on Beer Quality and Colloidal Stability

Ariola Devolli, Frederik Dara, Merita Stafasani, Edlira Shahinasi & Mariola Kodra

pp. 391 - 407   |  DOI: https://doi.org/10.29329/ijiaar.2018.174.12

Published online: December 17, 2018  |   Number of Views: 341  |  Number of Download: 939

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Abstract

Protein content and beer composition depend on the raw materials and enzymatic reactions used in brewery technology. In order to improve the colloidal stability of beer, it is necessary to remove both protein and polyphenolic complexes or prevent their formation. This study aims to determine the protein content in all production stages and to evaluate its influence on beer quality and colloidal stability.

Analyzes of total protein content were performed by Kjeldahl and spectrophotometric methods.

Statistically significant change was observed in the protein content of the final product, which was less than that in wort (P < 0.05). Turbididy, colour, extract, alcohol, bitterness, foam and pH were also measured.

Haze forcing tests (incubation at 200C, 400C and 600 C) were conducted to evaluate the colloidal stability of the final product. Results of tests showed that the highest level of product stability was ensured after treatment of beer with both silica gel and polyvinylpolypyrrolidone (PVPP).

Keywords: Beer quality, Colloidal stability, Protein content.

How to Cite this Article

APA 6th edition
Devolli, A., Dara, F., Stafasani, M., Shahinasi, E. & Kodra, M. (2018). The Influence of Protein Content on Beer Quality and Colloidal Stability. International Journal of Innovative Approaches in Agricultural Research, 2(4), 391-407. doi: 10.29329/ijiaar.2018.174.12

Harvard
Devolli, A., Dara, F., Stafasani, M., Shahinasi, E. and Kodra, M. (2018). The Influence of Protein Content on Beer Quality and Colloidal Stability. International Journal of Innovative Approaches in Agricultural Research, 2(4), pp. 391-407.

Chicago 16th edition
Devolli, Ariola, Frederik Dara, Merita Stafasani, Edlira Shahinasi and Mariola Kodra (2018). "The Influence of Protein Content on Beer Quality and Colloidal Stability". International Journal of Innovative Approaches in Agricultural Research 2 (4):391-407. doi:10.29329/ijiaar.2018.174.12.
References
  1. Aron, P.M. and Th.H. Shellhammer (2010). A Discussion of Polyphenols in Beer Physical and Flavour Stability. J. Inst. Brew. 116(4), 369–380. [Google Scholar]
  2. Bamforth, Ch. W. (1999). Beer haze. J. Am. Soc. Brew. Chem, 57, 81-90. [Google Scholar]
  3. Bamforth, Ch.W.  (2011). 125th Anniversary Review: The Non-Biological Instability of Beer, J. Inst. Brew. 117(4), 488–497. [Google Scholar]
  4. Briggs, D.E., C.A. Boulton, P.A. Brookes and R. Stevens (2004). Brewing: Science and Practice. Woodhead Publishing Limited: Cambridge, England, 881 p.  [Google Scholar]
  5. Callemien, D. and S. Collin (2008). Use of RP-HPLC-ESI(-)-MS/MS to differentiate various proanthocyanidin isomers in lager beer extracts. J. Am. Soc. Brew. Chem., 66(2), 109-115. [Google Scholar]
  6. Callemien, D., S. Guyot and S. Collin (2008). Use of thiolysis hyphenated to RP-HPLC-ESI (-)-MS/MS for the analysis of flavanoids in fresh lager beers. Food Chem., 110(4), 1012- 1018. [Google Scholar]
  7. Callemien, D. and S. Collin (2010). Structure, organoleptic properties, quantification methods, and stability of phenolic compounds in beer – a review. Food Rev. Int., 26,1–84. [Google Scholar]
  8. Clark, D.T. and C.W. Bamforth (2007). Realistic haze specifications for beer. Tech. Q. Master Brew. Assoc. Am., 44, 160-163 [Google Scholar]
  9. Collin, S., V. Jerkovic, M. Bröhan and D. Callemien (2013). Polyphenols and Beer Quality. In Natural Products: Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes; Ramawat, K.G., Mérillon, J.-M., Eds.; Springer: Berlin/Heidelberg, Germany, 2013; pp. 2333–2359.  [Google Scholar]
  10. Devolli, A., M. Kodra, E. Shahinasi and Dh. Feta (2015). Determination and elemination of beer turbidity.Proceeding Book ICAFE, 25 September 2015, Korçë Albania ISBN: 978-9928-146-41-0 [Google Scholar]
  11. Devolli, A., M. Kodra, E. Shahinasi, M. Stafasani and F. Dara (2017). Determination of Optimal Kieselguhr Doses to Improve Beer Filtration Proceeding Book ICFST, 27-29 Nobember 2017. Durres, Albania. ISBN 978-9951-437-63-9, 11-17. [Google Scholar]
  12. Dienstbier, M., P. Gabriel, P. Sladký and K. Sigler (2011). Prediction of Colloidal Stability of Highly Stabilized Beers by a Modified Chapon Tannoid Content Test, J. Inst. Brew. 117(3), 329–334. [Google Scholar]
  13. Dvorakova, M., P. Hulin, M. Karabin and P. Dostalek (2007). Determination of polyphenols in beer by an effective method based on solid-phase extraction and high performance liquid chromatography with diode-array detection. Czech J. Food Sci., 25(4), 182-188. [Google Scholar]
  14. European Brewery Convention (2008). Analytica EBC, Method 9.29, Haze in beer: calibration of haze meters; 9.30, Prediction of shelf-life of beer. Brauerei und Getränke Rundschau. 8th Ed. Fachverlag Hans Carl, Nürenberg.  [Google Scholar]
  15. European Brewery Convention (2007). Analytica EBC, Fachverlag Hans Carl, Nürnberg, Germany.  [Google Scholar]
  16. European Brewery Convention (1998). Analytica EBC, 5th ed., Section 9 Beer Method 9.6. Colour in Beer; 9.8. Bitterness in Beer; 9.11. Total polyphenols in Beer: Instrumental Method, Fachverlag Hans Carl, Nürnberg, Germany. [Google Scholar]
  17. Evans, D.E. and M.C. Sheehan (2002). Don’t be fobbed off: the substance of beer foam. J. Am. Soc. Brew. Chem., 60(2), 47–57 [Google Scholar]
  18. Gerhäuser, C. (2005). Beer constituents as potential cancer chemopreventive agents. Eur. J. Cancer, 41(13), 1941-1954. [Google Scholar]
  19. Goldammer, T. (2008). The Brewer's Handbook, Chapter 15 Beer Filtration Second Edition, ISBN (13): 978-0-9675212-3-7. [Google Scholar]
  20. Heverley, L., J. Leonard, L. Rusinski and C. Schuster, Colloidal Brew, www.eng.buffalo.edu/Courses/ce435/ce529/Project3/ColloidsInBeer.doc. [Google Scholar]
  21. Jones, B.L. and A.D. Budde (2005), How various malt endoproteinase classes affect wort soluble protein levels. J. Cereal Sci., 41(1), 95–106. [Google Scholar]
  22. Kunze, W. (2007). Technologie Brauer und Mälzer, VLB: Berlin. [Google Scholar]
  23. Leemans, C., J. Pellaud, L. Melotte and S. Dupire (2003). Opportunities for lag phase prediction: a new tool to assess beer colloidal stability. In: Proceedings of the 29th European Brewery Convention (Dublin), pp 88/1–88/11, Fachverlag Hans Carl, Nürnberg, Germany. [Google Scholar]
  24. Leiper, K.A., G.C. Stewart and I.P. McKeown (2003). Beer polypeptides and silica gel - Part I.Polypeptides involved in haze formation. J. Inst. Brew., 109, 57–72 [Google Scholar]
  25. Lewis, M.J. and T. W. Young (2002). Brewing. 2nd Edition. Kluwer Academic and Plenum Publishers: New York, 398 p.  [Google Scholar]
  26. Lingzhen Ye, Yuqing Huang, Fei Dai, Huajiang Ning, Chengdao Li, Meixue Zhou and Guoping Zhang (2015). Identification of two key genes controlling chill haze stability of beer in barley (Hordeum vulgare L). BMC Genomics, 16:449. [Google Scholar]
  27. Manual. (2017). Analysis Methods for the Brewery Industry, Spectroquant® Prove - Analysis Methods for the Brewery Industry.  [Google Scholar]
  28. Marković, R.S., O.S. Grujić and J.D. Pejin (2003). Conventional and alternative principles for stabilization of protein and polyphenol fractions in beer. APTEFF, 34, 1–148. [Google Scholar]
  29. MEBAK. (2002). Method 2.15.2. Forcier methode in MEBAK Brautechnische Analysen Methoden, Band II, 4. Aufgabe, H. Pfenninger Ed., Selbstverlag der MEBAK, Freizing-Weihenstephan. [Google Scholar]
  30. Nardini, M. and A. Ghiselli (2004). Determination of free and bound phenolic acids in beer. Food Chem., 84(1), 137-143. [Google Scholar]
  31. Robinson, L.H., D. Evan Evans, Anu Kaukovirta-Norja, Arvi Vilpola, Peter Aldred and Silja Home (2004). The Interaction Between Malt Protein Quality and Brewing Conditions and Their Impact on Beer Colloidal Stability. MBAA TQ vol. 41, no. 4, 353–362. [Google Scholar]
  32. Roza, J.R., C.E. Wallin and C. W. Bamforth (2006). A comparison between the instrumental measurement of head retention/lacing and perceived foam quality. Tech. Q. Master Brew. Assoc. Am., 43, 173-176. [Google Scholar]
  33. Siebert, K.J. (1999). Effects of protein-polyphenol interactions on beverage haze. Stabilization and analysis. J. Agric. Food Chem., 47, 353–362 [Google Scholar]
  34. Siebert, K.J. and P.Y. Lynn (1996). Formation of protein-polyphenol haze in beverages. J. Agric. Food Chem., 44(8): 1997–2005. [Google Scholar]
  35. Smythe, J.E., M.A. O’Mahony and C.W. Bamforth (2002). The Impact of the Appearance of Beer on Its Perception,” 108, no. 1 (January 2002). [Google Scholar]
  36. Steele, R. (2004). Understanding and measuring the shelf-life of food, Woodhead Publishing Series in Food Science Technology and Nutrition No. 100, 448 p. [Google Scholar]
  37. Steiner, E. and W. Back (2009). A critical review of protein assays and further aspects of new methods in brewing science. Brew Sci., 62, 90–94 [Google Scholar]
  38. Steiner, E., T. Becker and M. Gastl (2010). Turbidity and Haze Formation in Beer – Insights and Overview. J. Inst. Brew. 116(4), 360–368. [Google Scholar]
  39. Steiner, E., M. Gastl and T. Becker (2011). Protein changes during malting and brewing with focus on haze and foam formation: a review. Eur. Food Res. Technol., 232(2),191-204. [Google Scholar]
  40. Steiner, E., E.K. Arendt, T. Becker and M. Gastl (2011). Influence of the malting parameters on the haze formation of beer after filtration.  Eur. Food Res. Technol., 233, 587–597. [Google Scholar]
  41. Van Nierop, S.N., D.E. Evans, B.C. Axcell, I.C. Cantrell and M. Rautenbach (2004). Impact of different wort boiling temperatures on the beer foam stabilizing properties of lipid transfer protein 1. J. Agric. Food Chem., 52(10), 3120–3129. [Google Scholar]
  42. Wiesen, E., M. Gastl and Th. Becker (2011). Protein changes during malting and brewing with focus on haze and foam formation: A review. Eur. Food Res. Technol., 232(2), 191-204. [Google Scholar]
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