International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article    |    Open Access
International Journal of Innovative Approaches in Agricultural Research 2020, Vol. 4(2) 210-219

The Effects of Microbial Transglutaminase (MTG) Enzyme Usage on Sensory Properties of Hatay Cheese

Nuray Güzeler, Hüseyin Mert & Çağla Özbek

pp. 210 - 219   |  DOI: https://doi.org/10.29329/ijiaar.2020.254.5

Published online: June 29, 2020  |   Number of Views: 176  |  Number of Download: 651


Abstract

Hatay Cheese is a kind of cheese produced in and around Hatay with its own shapes and sizes. In this study, the use of microbial transglutaminase enzyme was tried to improve the textural structure of Hatay cheese which is in fresh cheese class. Enzymatic modification of milk proteins by microbial transglutaminase enzyme has been applied in the production of half-fat Hatay cheese with different pre-ripening time applications and some sensory properties of the cheeses have been investigated such as external appearance, internal appearance, texture, odor and taste during 60 days of storage. In this study, four different Hatay cheese were produced as control sample (A), 30 minutes pre-ripening with enzyme (B), 45 minutes pre-ripening with enzyme (C) and 60 minutes pre-ripening with enzyme (D).

When the sensory properties of the cheeses were examined, it was observed that the application of different pre-ripening time significantly affected the external appearance, internal appearance, texture and taste scores on the 60th day of storage (p<0.05), and storage time caused significant differences on the appearance scores of A, B and C cheeses (p<0.05). Storage time caused significant differences on the internal appearance scores of all cheeses except cheese A (p<0.05). The effects of storage time on texture scores were not significant (p>0.05). The effects of different pre-ripening periods and storage time on the odor scores of the cheeses were not significant (p>0.05). Taste scores of cheese D were significantly affected by storage time (p<0.05). As a result of all sensory analyzes, it was found that the cheeses supplemented with MTG enzyme were more liked and the scores given to the cheeses increased as the pre-ripening period increased. As a result, the most admired cheese was D cheese.

Keywords: Microbial transglutaminase, Hatay cheese, Sensory properties


How to Cite this Article

APA 6th edition
Guzeler, N., Mert, H. & Ozbek, C. (2020). The Effects of Microbial Transglutaminase (MTG) Enzyme Usage on Sensory Properties of Hatay Cheese . International Journal of Innovative Approaches in Agricultural Research, 4(2), 210-219. doi: 10.29329/ijiaar.2020.254.5

Harvard
Guzeler, N., Mert, H. and Ozbek, C. (2020). The Effects of Microbial Transglutaminase (MTG) Enzyme Usage on Sensory Properties of Hatay Cheese . International Journal of Innovative Approaches in Agricultural Research, 4(2), pp. 210-219.

Chicago 16th edition
Guzeler, Nuray, Huseyin Mert and Cagla Ozbek (2020). "The Effects of Microbial Transglutaminase (MTG) Enzyme Usage on Sensory Properties of Hatay Cheese ". International Journal of Innovative Approaches in Agricultural Research 4 (2):210-219. doi:10.29329/ijiaar.2020.254.5.

References
  1. Abou-Soliman, N.H.I., S. Awad and M. E. El-Sayed  (2020). The impact of microbial transglutaminase on the quality and antioxidant activity of camel-milk soft cheese. Food  Nutr. Sci., 11, 153-171. [Google Scholar]
  2. Al-Otaibi, M. M., and R. A. Wilbey (2005). Effect of chymosin and salt reduction on the quality of ultrafiltrated white-salted cheese. J. Dairy Res., 72(2), 234-242. [Google Scholar]
  3. Anonymous (2010). Sensory evaluation. British Nutrition Foundation, http://www.foodafactoflife.org.uk/attachments/6f6c51d7-d7b6-40053a38a7f2.pdf, Access date: 15.02.2018. [Google Scholar]
  4. Anonymous (2018). ACTIVA® Transglutaminase. http://www.ajinomoto.de/cms/front_content.php?idcat=76, Access date: 21.03.2018. [Google Scholar]
  5. Anonymous (2001). Transglutaminase GRAS notification submitted to the US. Food and Drug Administration, p. 200.   [Google Scholar]
  6. Anonymous, (2015). Türk Gıda Kodeksi, Peynir Tebliği. Resmi Gazete. Sayı: 29261 Gıda, Tarım ve Hayvancılık Bakanlığı, Tebliğ no: 2015/6.    [Google Scholar]
  7. Badem, A., (2015). Rennet kazeinin kaşar peynirinin kimyasal, mikrobiyolojik ve duyusal kalite niteliklerine etkisi. Doktora Tezi, Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü Besin Hijyeni ve Teknolojisi Anabilim Dalı, Konya, s.114.  [Google Scholar]
  8. Bulut Solak, B. (2013). Farklı tip peynirler kullanılarak üretilen eritme tipi peynirlerin üretimi esnasında uygulanan işlem parametrelerinin peynirin bazı özellikleri üzerine etkisi. Doktora Tezi, Selçuk Üniversitesi Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı, Konya, s.313. [Google Scholar]
  9. Carbonell-Barrachina, A.A. (2007). Application of sensory evaluation of food to quality control in the Spanish food industry. Polish J. Food Nutr. Sci., 57 (4A), 71-76. [Google Scholar]
  10. Çayır, M.S. (2018). İnek, keçi sütü ve karışımlarından üretilen hatay köy peynirlerinin depolama süresince bazı kalite kriterlerinin belirlenmesi. Doktora Tezi, Çukurova Üniversitesi Gıda Mühendisliği Anabilim Dalı, Adana, s.170. [Google Scholar]
  11. Danesh, E., H. Jooyandeh and M.  Goudarzi (2017). Improving the rheological properties of low-fat Iranian UF-feta cheese by incorporation of whey protein concentrate and enzymatic treatment of transglutaminase. Iranian J. Food Sci. Tech., 14(67):285-298. [Google Scholar]
  12. Darnay, L., A. Toth, B.  Salamon, K.  Papik, G. Oros, G. Jonas, K. Horti, K. Koncz and L. Friedrich  (2017). Texture-modifying properties of microbial transglutaminase on 2 popular Hungarian products: Trappist cheese and Frankfurter. Acta Aliment., 46 (1): 116–122. [Google Scholar]
  13. Ehsannia, S. and  M. R. Sanjabi (2016). Quality characterization of processed cheese inoculated by Bacillus coagulans during cold storage: compositional and sensorial attributes and probiotic microorganism viability. J. Food Process. Pres., 40, 667–674. [Google Scholar]
  14. Ercili-Cura, D. (2012). Structure modification of milk protein gels by enzymatic cross-linking. VTT Science 24, p. 82. [Google Scholar]
  15. Gan, C.Y., L.H. Cheng and A. M. Easa (2008). Physicochemical properties and microstructures of soy protein isolate gels produced using combined cross-linking treatments of microbial transglutaminase and Maillard cross-linking. Food Res. Int., 41, 600–605. [Google Scholar]
  16. Gan, C.Y., A. A. Latiff, L. H. Cheng and A. M. Easa (2009). Gelling of microbial transglutaminase cross-linked soy protein in the presence of ribose and sucrose. Food Res. Int., 42, 1373–1380. [Google Scholar]
  17. Garcia-Gomez, B., L. Vazquez-Oderiz, N. Munoz-Ferreiro, A. Romero-Rodriguez and M. Vazquez (2020). Rennet type and microbial transglutaminase in cheese: effect on sensory properties. Eur. Food Res. Technol., 246, 513–526. [Google Scholar]
  18. Gharibzahedi, S.M.T., M. Kouba, F. J.  Barba, R. Greiner, S. George and S. Roohinejad (2018). Recent advances in the application of microbial transglutaminase crosslinking in cheese and ice cream products: A review. Int. J. Biol. Macromolec., 107, 2364-2374. [Google Scholar]
  19. Karzan, T.M., H. S. Nawal and T. A. Ashna (2016). The effect of microbial transglutaminase enzyme on some physicochemical and sensory properties of goat’s whey cheese. Int. Food Res. J. 23(2), 688-693. [Google Scholar]
  20. Kesenkaş, H., N. Dinkçi and Ö. Kınık (2012). Farklı işletmelerde üretilen köy peynirlerinin özellikleri. Ege Üniversitesi Ziraat Fakültesi Dergisi, 49(2), 167-173. [Google Scholar]
  21. Kondyli, E., E.C. Pappa and C. Svarnas (2016). Ripening changes of the chemical composition, proteolysis, volatile fraction and organoleptic characteristics of a white-brined goat milk cheese. Small Rum. Res., 145, 1–6. [Google Scholar]
  22. Kuraishi, C., Y. Katsutoshi and Y. Susa (2001).  Transglutaminase:  its utilization in the food industry. Food Rev. Int., 17(2), 221-246. [Google Scholar]
  23. Mahmood, W.A. and N. H. Sebo (2009). Effect of microbial transglutaminase treatment on soft cheese properties. Mesopotamia J. Agric., 37(4), 1-9. [Google Scholar]
  24. Miloradovic, Z., N. Kljajevic, J. Miocinovic, N. Tomic, J. Smiljanic and O. Macej (2017). High heat treatment of goat cheese milk the effect on yield, composition, proteolysis, texture and sensory quality of cheese during ripening. Int. Dairy J., 68, 1-8 [Google Scholar]
  25. Motoki, M. and Y. Kumazawa (2000). Recent research trends in transglutaminase technology for food processing. Food Sci. Technol. Res., 6 (3), 151-160.        [Google Scholar]
  26. Motoki, M. and K. Seguro (1998). Transglutaminase and its use for food processing. Trend. Food Sci.Technol., 9, 204–210. [Google Scholar]
  27. Paksoy, G. (2016). Bazı baharatların ultrafiltre beyaz peynir kalitesi üzerine etkileri. Yüksek Lisans Tezi, Namık Kemal Üniversitesi Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı, Tekirdağ, s.63. [Google Scholar]
  28. Rahila, M.P., R. Kumar, P. S. Rao and B. Mann (2015). Transglutaminase cross- linking of milk proteins for improved functionality of cow and buffalo milk. Indian J. Dairy Sci., 68(4). [Google Scholar]
  29. Romeih, E. and G. Walker  (2017). Recent advances on microbial transglutaminase and dairy application. Trends Food Sci. Technol., 62, 133-140. [Google Scholar]
  30. TSE, (2013). TS 591. Beyaz Peynir Standardı. Türk Standartları Ensititüsü, Ankara. [Google Scholar]
  31. Yokoyama, K., H. Utsumi, T. Nakamura, D. Ogaya, S. Nobuhisa, E. Suzuki  and S. Taguchi (2010). Screening for improved activity of a transglutaminase from Streptomyces mobaraensis created by a novel rational mutagenesis and random mutagenesis. Appl. Microbiol. Biotech., 87(6), 2087-2096.   [Google Scholar]