International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article    |    Open Access
International Journal of Innovative Approaches in Agricultural Research 2022, Vol. 6(4) 417-425

Effect of the Dietary Royal Jelly Supplementation in Ewes of Bulgarian Dairy Synthetic Population on the Body Weight of the Lambs and the Milk Composition

Tanya Ivanova, Teodora Popova & Ralitsa Balkanska

pp. 417 - 425   |  DOI:

Published online: December 31, 2022  |   Number of Views: 13  |  Number of Download: 56


The aim of the experiment was to assess the effect of the royal jelly supplementation in the diet of ewes from Bulgarian Dairy Synthetic Population on the body weight of the lambs and the milk composition.  The trial was carried out with 20 ewes and their lambs, reared in the experimental sheep farm of the Institute of Animal Science- Kostinbrod, Bulgaria and lasted 30 days during the suckling period. The animals had the same date of lambing and lambs’ body weight, and were divided into two groups, each containing 10 ewes. The feeding was according to the standards for this category of ruminants with ad libitum access to water, as each ewe from the supplemented group received twice a week 300 mg freeze dried royal jelly.  The body weight of the lambs was controlled at the beginning and at the end of the trial period, and the weight gain was calculated. The chemical and the fatty acid composition of the milk was measured in individual samples. The fatty acid composition was used to calculate the atherogenic (AI) and thrombogenic (TI) indices. The live weight (LW) and the average daily gain (ADG) differed between the groups of lambs according to the dietary royal jelly supplementation. The lambs of the ewes that had received the supplement displayed higher LW (P=0.0138) and ADG (P=0.0062).  The physicochemical composition of the milk showed lower fat content (P=0.0142) and higher density (P=0.0200) in the ewes receiving royal jelly. Significant increase of C18:0 and decrease of C17:1 proportion was observed. Additionally, the contents of the polyunsaturated С20:4n-6, С22:5n-3 and C22:6n-3 was considerably diminished in the milk of the group that received royal jelly. The results so far demonstrated the potential of the royal jelly to manipulate the fatty acid profile of the ewes’ milk.

Keywords: Royal Jelly, Dairy Sheep, Sucking Period, Body Weight, Milk Composition

How to Cite this Article

APA 6th edition
Ivanova, T., Popova, T. & Balkanska, R. (2022). Effect of the Dietary Royal Jelly Supplementation in Ewes of Bulgarian Dairy Synthetic Population on the Body Weight of the Lambs and the Milk Composition . International Journal of Innovative Approaches in Agricultural Research, 6(4), 417-425. doi: 10.29329/ijiaar.2022.506.12

Ivanova, T., Popova, T. and Balkanska, R. (2022). Effect of the Dietary Royal Jelly Supplementation in Ewes of Bulgarian Dairy Synthetic Population on the Body Weight of the Lambs and the Milk Composition . International Journal of Innovative Approaches in Agricultural Research, 6(4), pp. 417-425.

Chicago 16th edition
Ivanova, Tanya, Teodora Popova and Ralitsa Balkanska (2022). "Effect of the Dietary Royal Jelly Supplementation in Ewes of Bulgarian Dairy Synthetic Population on the Body Weight of the Lambs and the Milk Composition ". International Journal of Innovative Approaches in Agricultural Research 6 (4):417-425. doi:10.29329/ijiaar.2022.506.12.

  1. Aguiar, S.C., Cottica, S.M., Boeing, J.S., Samensari, R.B., Santos, G.T., Visentainer, J.V., Zeoula, L.M., 2014. Effect of feeding phenolic compounds from propolis extracts to dairy cows on milk production, milk fatty acid composition, and the antioxidant capacity of milk. Animal Feed Science and Technology, 193: 148 – 154, [Google Scholar] [Crossref] 
  2. Babaei, S., Rahimi, S., Karimi Torshizi, M.A., Tahmasebi, G., Khaleghi Miran, S.N., 2016. Effects of propolis, royal jelly, honey and bee pollen on growth performance and immune system of Japanese quails. Veterinary Research Forum, 7(1):13-20. [Google Scholar]
  3. Christie, W.W. 1973. Lipid analysis. Pergamon Press. Oxford. 352 p. [Google Scholar]
  4. Domínguez, R., Crecente, S., Borrajo, P., Agregan, R., Lorenzo, J.M., 2015. Effect of slaughter age on foal carcass traits and meat quality, Animal, 9: 1713-1720, [Google Scholar] [Crossref] 
  5. El-Tarabany, M.S., El-Tarabany, A.A., Atta, M.A., Ahmed-Farid, O.A., Mostafa, M.M., 2019. Effect of royal jelly on milk composition and blood biochemical pаrameters in lactating ewes. The Journal of Agricultural Science, 157 (2):1–6, [Google Scholar] [Crossref] 
  6. El-Tarabany, А.А.S, Ahmed-Farid, O.A., Emara, S.S., El-Tarabany., M.S., 2020. Impact of royal jelly supplement on ewe's milk fat content and fatty acid profile during the early stage of lactation. Advances in Applied Physiology, 5(2): 37-41, [Google Scholar] [Crossref] 
  7. Gerchev, G., Naydenova, N., Slavkova, S., Mihaylova, G., 2015. Fatty acid composition of milk fat in milk of Tzigay sheep and their F2 cross-breeds with Chios. Biotechnology in Animal Husbandry, 31(1), 45-53, [Google Scholar] [Crossref] 
  8. Hara, A., Radin, N.S., 1978. Lipid extraction of tissues with a low-toxicity solvent. Analytical Biochemistry, 90: 420-426, [Google Scholar] [Crossref] 
  9. Husein, M.Q., Kridli, R.T., Humphrey, W.D., 1999. Effect of royal jelly on estrus synchronization and pregnancy rate of ewes using flourogestone acetate sponges. Journal of Animal Science, 77, 431-438. [Google Scholar]
  10. Ivanova, T., Raicheva, E.,  Pacinovski, N., 2011. Yield of cheese and milk composition in sheep from Synthetic Population Bulgarian Milk and East-Friesian breed. Biotechnology in Animal Husbandry, 27(3): 1105-1111, [Google Scholar] [Crossref] 
  11. Kridli, R.T., Husein, M.Q., Humphrey, W. D.,  2003. Effect of royal jelly and GnRH on the estrus synchronization and pregnancy rate in ewes using intravaginal sponges. Small Ruminant Research, 49(1): 25–30, [Google Scholar] [Crossref] 
  12. Kridli, R.T., Al-Khetib, S.S., 2006. Reproductive responses in ewes treated with eCG or increasing doses of royal jelly. Animal Reproduction Science, 92(1–2): 75-85, [Google Scholar] [Crossref] 
  13. Madras-Majewska, B., Ochnio, L., Ochnio, M., 2015. Use of bee products in livestock nutrition and therapy. Medycyna Weterynaryjna, 71 (2): 94-99. [Google Scholar]
  14. Moradi, А. R., Malekinejad, H., Farrokhi-Ardabili, F., Bernousi, I., 2013. Royal Jelly improves the sperm parameters of ram semen during liquid storage and serves as an antioxidant source. Small Ruminant Research, 113(2–3): 346-352, [Google Scholar] [Crossref] 
  15. Muqier, Qi, S.,Wang, T., Chen, R., Wang, C., Ao, C., 2017.  Effects of flavonoids from Allium mongolicum Regel on growth performance and growth-related hormones in meat sheep. Animal Nutrition, 3 (1): 33-38, [Google Scholar] [Crossref] 
  16. Park, J.-H., Shanmugam, S., Ho, K. I., 2020. Influences of dietary flavonoid (quercetin) supplementation on growth performance and immune response of growing pigs challenged with Escherichia coli lipopolysaccharide. Journal of Animal Science and Technology, 62(5):605-613, [Google Scholar] [Crossref] 
  17. Pavel, C. I., L. Al. Marghitas, O. Bobis, D. S. Dezmirean, A. Sapcaliu, I. Radoi, M. N. Madas. 2011. Biological activities of royal jelly – review. Scientific Papers: Animal Science and Biotechnologies, 44(2): 108 – 118. [Google Scholar]
  18. Prihambodo, T.R., M.M. Sholikin, N. Qomariyah, A. Jayanegara, I. Batubara, D.B. Utomo, N. Nahrowi, 2021. Effects of dietary flavonoids on performance, blood constituents, carcass composition and small intestinal morphology of broilers: a meta-analysis. Animal Bioscience, 34(3):434-442, [Google Scholar] [Crossref] 
  19. Seven, I., Aksu, T., Tatli Seven, P., 2012. The effects of propolis and vitamin C supplemented feed on performance, nutrient utilization and carcass characteristics in broilers exposed to lead. Livestock Science, 148(1 – 2): 10 – 15, [Google Scholar] [Crossref] 
  20. Seven, I., Şimşek, Ü. G., Gökçe, Z., Seven, P. T., Arslan, A., Yilmaz, Ö., 2014. The effects of royal jelly on performance and fatty acid profiles of different tissues in quail (Coturnix coturnix japonica) reared under high stocking density. Turkish Journal of Veterinary and Animal Sciences, 38(3): 271 – 277, [Google Scholar] [Crossref] 
  21. Seven, P.T., A. Sur Arslan, M. Özçelik, Ü. Gülcihan Şimşek, İ. Seven, 2016. Effects of propolis and royal jelly dietary supplementation on performance, egg characteristics, lipid peroxidation, antioxidant enzyme activity and mineral levels in Japanese quail. European Poultry Science, 80, [Google Scholar] [Crossref] 
  22. Sinanoglou, V. J., P. Koutsouli, C. Fotakis, G. Sotiropoulou, D. Cavouras, Iosif Bizelis. 2015. Assessment of lactation stage and breed effect on sheep milk fatty acid profile and lipid quality indices. Dairy Science & Technology, 95(4): 509-531, [Google Scholar] [Crossref] 
  23. Strant, M., Yücel, B., Topal E., Puscasu A.M., Margaoan, R., Varadi, A., 2019. Use of royal jelly as functional food in human and animal health. J. Anim. Prod., 60(2), 131-144, [Google Scholar] [Crossref] 
  24. Ulbricht, T.L.  Southgate, D.A.T., 1991. Coronary heart disease: seven dietary factors. Lancet, 338: 985–992, [Google Scholar] [Crossref] 
  25. Voblikova, T., Permyakov, А., Rostova, А., Masyutina, G., Eliseeva, A. 2020. Study of fatty-acid composition of goat and sheep milk and its transformation in the production of yogurt. International Applied Research Conference “Biological Resources Development and Environmental Management”, KnE Life Sciences, 742-751, [Google Scholar] [Crossref]