International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article | International Journal of Innovative Approaches in Agricultural Research 2021, Vol. 5(4) 476-483

The Effects of In Ovo Injection of Propolis Extract to Breeder Eggs on Hatchability and Early Performance in Broilers

Berceste Babaeker, Çiğdem Şeremet Tuğalay & Özer Hakan Bayraktar

pp. 476 - 483   |  DOI: https://doi.org/10.29329/ijiaar.2021.415.12

Published online: December 31, 2021  |   Number of Views: 6  |  Number of Download: 79


Abstract

This research was conducted to determine the effects of in ovo injection of propolis extract into the amnion to breeder eggs on hatchability and early performance in broilers. For this purpose, a total of 250 Ross 308 broiler breeder hatching eggs were obtained from a commercial hatchery. Eggs were divided into four groups randomly, isotonic serum sodium (0.9%) was injected at the level of 0.2 ml into the first group and there were used as positive control (PC) group, whereas there was no treatment on negative control (NC) group. Where the amount of 0.2 ml prepared mixture which is included %3 propolis extract were injected into low propolis (LP) group, 0.2 ml prepared mixture which is included %6 propolis extract were given into the high propolis group (HP). In ovo propolis extract injection resulted in a shorter hatching time compared with the control groups. The hatchability, hatching weight and survival of newly hatched chicks were not affected by in ovo injection of propolis extract according to our results. There were no differences between the control and treatmet groups in hatchability and embryonic mortality. The results indicated that except for the PC group chicks’ weights were similar at hatching day, but the later stage weights of chicks were started to significantly differ from each other and propolis groups have shown higher live weights than the controls at 5th and 10th day (P<0.05). All treatment groups exhibited greater weight gain from hatch to 10 d compared to the control groups. The results indicate that in ovo feeding of broiler embryos with propolis extract may have beneficial effects on day old chick weights and early growth rate.

Keywords: Broiler, Hatching, In ovo injection, Performance, Propolis


How to Cite this Article?

APA 6th edition
Babaeker, B., Tugalay, C.S. & Bayraktar, O.H. (2021). The Effects of In Ovo Injection of Propolis Extract to Breeder Eggs on Hatchability and Early Performance in Broilers . International Journal of Innovative Approaches in Agricultural Research, 5(4), 476-483. doi: 10.29329/ijiaar.2021.415.12

Harvard
Babaeker, B., Tugalay, C. and Bayraktar, O. (2021). The Effects of In Ovo Injection of Propolis Extract to Breeder Eggs on Hatchability and Early Performance in Broilers . International Journal of Innovative Approaches in Agricultural Research, 5(4), pp. 476-483.

Chicago 16th edition
Babaeker, Berceste, Cigdem Seremet Tugalay and Ozer Hakan Bayraktar (2021). "The Effects of In Ovo Injection of Propolis Extract to Breeder Eggs on Hatchability and Early Performance in Broilers ". International Journal of Innovative Approaches in Agricultural Research 5 (4):476-483. doi:10.29329/ijiaar.2021.415.12.

References
  1. Aygun, A. (2016). The effects of in-ovo injection of propolis on egg hatchability and starter live performance of Japanese quails. Brazilian Journal of Poultry Science, 2, 83-89. [Google Scholar]
  2. Bello, A., Zhai, W., Gerard, P.D. & Peebles, E.D. (2013). Effects of the commercial in ovo injection of 25-hydroxycholecalciferol on the hatchability and hatching chick quality of broilers. Poultry Science, 92, 2551-2559. [Google Scholar]
  3. Bhanja, S.K., Mandal, A.B., Agarwal, S.K., Majumdar S. & Bhattacharyya, A. (2007). Effect of in ovo injection of vitamins on the chick weight and post-hatch growth performance in broiler chickens. European Symposium Poultry Nutrition, 16, 143-146. [Google Scholar]
  4. Bhargava, K.K., Hanson, R.P. & Sunde, M.L. (1971). Effects of threonine on growth and antibody production in chicks infected with live or killed New Castle Disease virus. Poultry Science, 50, 710-713. [Google Scholar]
  5. Dalloul, R.A., Lillehoj, H.S., Klinman, D.M., Ding, X., Min, W., Heckert, R.A. & Lillehoj, E.P. (2005). In ovo administration of CpG oligodeoxynucleotides and the recombinant microneme protein MIC2 protects against Eimeria infections. Vaccine, 23, 3108-3113. [Google Scholar]
  6. Decuypere, E. (1979). Effect of incubation temperature patterns on morphological, physiological and reproduction criteria in Rhode Island Red birds. Agricultura, 27, 216-230. [Google Scholar]
  7. Deeming, D.C. (1995). What is chick quality? World Poultry, 11,2, 20-23. [Google Scholar]
  8. Druyan, S. (2010). The effects of genetic line (broilers vs. layers) on embryo development. Poultry Science, 89, 1457-1467. [Google Scholar]
  9. Gore, A.B. & Qureshi, M.A. (1997). Enhancement of humoral and cellular immunity by vitamin E after embryonic exposure. Poultry Science, 76, 984-991. [Google Scholar]
  10. Hager, J.E. & Beane W.L. (1983). Post-hatch incubation time and early growth of broiler chickens. Poult. Sci. 62, 247-254. [Google Scholar]
  11. Henry, M.H. & Burke, W.H. (1999). The effects of in ovo administration of testosterone or an antiandrogen on growth of chick embryos and embryonic muscle characteristics. Poultry Science, 78, 1006-1013. [Google Scholar]
  12. Johnson, R. (2018). In ovo feeding: ingesting the facts. https://www.thepoultrysite.com/articles/in-ovo-feeding-ingesting-the-facts. Accessed 18 July 2018. [Google Scholar]
  13. Kadam, M.M., Bhanja, S.K., Mandal, A.B., Thakur, R., Vasan, P., Bhattacharyya, A. & Tyagi, J.S. (2008). Effect of in ovo threonine supplementation on early growth, immunological responses and digestive enzyme activities in broiler chickens. British Poultry Science, 49, 736-741. [Google Scholar]
  14. Keralapurath, M.M., Corzo, A., Pulikanti, R., Zhai, W. & Peebles, E.D. (2010). Effects of in ovo injection of l–carnitine on hatchability and subsequent broiler performance and slaughter yield. Poultry Science, 89, 1497-1501. [Google Scholar]
  15. Kılıç Altun, S. & Aydemir, M.E. (2020). Phenolic characterization of some propolis samples of Anatolia. Journal of Bahri Dagdas Animal Research, 9,2, 97-104. [Google Scholar]
  16. Kocamis, H., Yeni, Y.N., Brown, C.U., Kenney, P.B., Kirkpatrick-Keller, D.C. & Killefer, J. (2000). Effect of in ovo administration of insulin-like growth factor-I on composition and mechanical properties of chicken bone. Poultry Science, 79, 1345-1350. [Google Scholar]
  17. Kop Bozbay, C., Konanç, K., Ocak, N., Öztürk, E. (2016). The effects of in ovo injection of propolis and injection site on hatchability, hatching weight and survival of chicks. Turkish Journal of Agricultural Research, 3,1, 48-54. [Google Scholar]
  18. Kordali, N. (2020). In-Ovo Nutrition: a review. Pen & Tec Consulting. https://pentec-consulting.eu/in-ovo-nutrition-a-review/. Accessed 02 March 2020. [Google Scholar]
  19. Kucharska-Gaca, J., Kowalska, E. & Dębowska, M. (2007). Feeding-technology of the future - A Review. Annals of Animal Science, 17, 4, 979-99. [Google Scholar]
  20. McGruder, B.M., Zhai, W., Keralapurath, M.M., Bennett, L.W., Gerard, P.D. & Peebles, E.D. (2011). Effects of in ovo injection of electrolyte solutions on the pre- and post-hatch physiological characteristics of broilers. Poultry Science, 90, 1058-1066. [Google Scholar]
  21. Ohta, Y., Tsushima, N., Koide, K., Kidd, M.K. & Ishibashi, T. (1999). Effect of amino acid injection in broiler breeder eggs on embryonic growth and hatchability of chicks. Poultry Science, 78, 1493-1498. [Google Scholar]
  22. Peebles, E.D. (2018). In ovo applications in poultry: A review Poultry Science, 97, 2322-2338. [Google Scholar]
  23. Ramos, A.F.N. & Miranda, J.L. (2007). Propolis: a review of its anti-inflammatory and healing actions. Journal of Venomous Animals and Toxins including Tropical Diseases, 13, 697-710. [Google Scholar]
  24. Romanoff, A.L. & Hayward, F.W. (1943). Changes in volume and physical properties of allantoic and amniotic fluids under normal and extreme temperatures. Biological Bulletin, 84, 2, 141-147. [Google Scholar]
  25. JMP. Version 10. SAS Institute Inc., Cary, NC. (1989–2021). [Google Scholar]
  26. Sanniyasi, B., Bhanja, S., Majumdar, S. & Banabihari, D. (2012). Post-hatch immunomodulation through in ovo supplemented nutrients in broiler chickens. Journal of the Science of Food and Agriculture, 92, 313-320. [Google Scholar]
  27. Sharma, J.M. & Burmester, B.R. (1982). Resistance to Marek’s disease at hatching in chickens vaccinated as embryos with the turkey herpesvirus. Avian Diseases, 26, 134-149. [Google Scholar]
  28. Tako, E., Ferket, P.R. & Uni, Z. (2004). Effects of in ovo feeding of carbohydrates and β-hydroxy-β-methylbutyrate on the development of chicken intestine. Poultry Science, 83, 2023-2028. [Google Scholar]
  29. Tona, K., Onagbesan, O., De Ketelaere, B., Bruggeman, V. & Decuypere, E. (2005). Interrelationships between chick quality parameters and the effect of individual parameter on broiler relative growth to 7 days of age. Archive fur Geflügelkunde, 69,2, 67-72. [Google Scholar]
  30. Uni, Z. & Ferket, R.P. (2004). Methods for early nutrition and their potential. World’s Poultry Science Journal, 60, 101-111. [Google Scholar]
  31. Uni, Z., Ferket, P.R., Tako, E. & Kedar, O. (2005). In ovo feeding improves energy status of late-term chicken embryos. Poultry Science, 84, 764-770. [Google Scholar]
  32. Wang, J.S., Guo, T.Y., Wang, Y.X., Li, K.X., Wang, Q. & Zhan, X.E. (2018) Effects of first feed intake time on growth performance, nutrient apparent metabolic rate and intestinal digestive enzyme activities in broilers. Asian-Australian Journal of Animal Science, 31, 6, 899-904. [Google Scholar]
  33. Zhai, W., Neuman, S., Latour, M.A. & Hester, P.Y. (2008). The effect of in ovo injection of l-carnitine on hatchability of White Leghorns. Poultry Science, 87, 569-572. [Google Scholar]
  34. Zuidhof, M.J., Schneider, B.L., Carney, V.L., Korver, D.R. & Robinson, F.E. (2014). Growth, efficiency, and yield of commercial broilers from 1957, 1978, and 2005. Poultry Science, 93, 1-13. [Google Scholar]