International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article | International Journal of Innovative Approaches in Agricultural Research 2021, Vol. 5(4) 381-389

Bacillus Strains as an Effective Treatment of Mobile Forms of Phosphorus in Bulgarian Soils

Tsvetana Licheva, Dilyana Nikolova & Evstatieva Yana

pp. 381 - 389   |  DOI: https://doi.org/10.29329/ijiaar.2021.415.4

Published online: December 31, 2021  |   Number of Views: 5  |  Number of Download: 112


Abstract

Strains of genus Bacillus have the potential to increase the availability of phosphorus to plants by the dissolution of inorganic phosphate, which favours the growth of plant species and that has an important economic and agricultural impact. Soils with low phosphate intensity (Lеаched chernozem) were enriched with poorly soluble phosphorus compounds and inoculated with the tested newly isolated strains from genus Bacillus (Bacillus subtilis T 2, Bacillus amyloliquefaciens T 3, Bacillus subtilis T 4, Bacillus subtilis T 10, Bacillus thuringiensis T 17 and Bacillus cereus Т 18). Six newly isolated strains from different regions in Bulgaria were identified by classical phenotypic techniques and 16S rDNA sequence analysis. Tested strains were inoculated (2 ml and 15 ml) in the soil and incubated for 25 days at 28oC. After the incubation period, the degradation of phosphorite flour to available phosphorus was examined by the classical method of Egner-Riehm and by extraction with CaCl2.

A vegetation experiment was conducted with a test plant Pelargonium zonale, characterized by its ability to absorb large amounts of phosphorus. A peat substrate enriched with all macro-and microelements was used, and phosphorus was added to the medium in the form of phosphorite flour. The amounts of phosphorus absorbed by one plant Pelargonium zonale treated with newly isolated strains Bacillus subtilis T 10 and Bacillus cereus T 18 absorbed 23% more phosphorus than the control variant.

 As a result of the experiments, it was found that the studied strains have a positive impact on the increase of phosphorus mobility in soils with low phosphate intensity treated with hardly degradable phosphors.

Keywords: Bacillus, Lеаched chernozem, Phosphorus, Pelargonium zonale


How to Cite this Article?

APA 6th edition
Licheva, T., Nikolova, D. & Yana, E. (2021). Bacillus Strains as an Effective Treatment of Mobile Forms of Phosphorus in Bulgarian Soils . International Journal of Innovative Approaches in Agricultural Research, 5(4), 381-389. doi: 10.29329/ijiaar.2021.415.4

Harvard
Licheva, T., Nikolova, D. and Yana, E. (2021). Bacillus Strains as an Effective Treatment of Mobile Forms of Phosphorus in Bulgarian Soils . International Journal of Innovative Approaches in Agricultural Research, 5(4), pp. 381-389.

Chicago 16th edition
Licheva, Tsvetana, Dilyana Nikolova and Evstatieva Yana (2021). "Bacillus Strains as an Effective Treatment of Mobile Forms of Phosphorus in Bulgarian Soils ". International Journal of Innovative Approaches in Agricultural Research 5 (4):381-389. doi:10.29329/ijiaar.2021.415.4.

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