Pen Academic Publishing   |  e-ISSN: 2602-4772

Original article | International Journal of Innovative Approaches in Agricultural Research 2019, Vol. 3(4) 550-556

Investigation of the Anticancer and Proliferative Effect of Broccoli Extract on Du145 Prostate Cancer and MEF Healthy Fibroblast Cell Lines

Nebiye Pelin Türker, Ufuk Bağcı & Pelin Onsekizoglu Bagci

pp. 550 - 556   |  DOI: https://doi.org/10.29329/ijiaar.2019.217.1   |  Manu. Number: MANU-1905-20-0007

Published online: December 10, 2019  |   Number of Views: 32  |  Number of Download: 146


Abstract

In recent years, many researchers have focused on the health effects of plant-derived foods. In this context, foods with high content of flavonoids and phenolic substances have received a great deal of attention as potential agents for cancer prevention and treatment. Studies on the broccoli plant have revealed that broccoli has antioxidant and anti-carcinogenic properties. However, effective dose of broccoli needs to be determined for the cancer treatment. In this study, the effects of broccoli extracts on du145 prostate cancer and Mef fibroblast healthy cells were investigated at different doses for 48 hours.

Du145 and Mef cells were grown with Dulbecco's Minimum Essential Medium (DMEM) and HAMS F 12 (1: 1) supplemented with 2% FBS. Broccoli extracts at ten different doses (0,19% - 100%) were added into cultures and incubated at 37°C for 24 and 48 h in 5% CO2. The viability of the cells was determined by the MTT method (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide). Probit analysis by SPSS software revealed that the broccoli extract at a dose of 4.070 % dose killed du145 cancer cells at 48 h. At the same time, MTT results showed that the viability of Mef cells was increased during 48 h of incubation.

As a result, broccoli extract showed a significant level of anticancer activity in Du145 cells, while increasing the viability of Mef healthy cells. This result suggests that broccoli extract is a potential candidate for cancer treatment.

Keywords: broccoli, cancer, prostate cancer, fibroblast cell


How to Cite this Article?

APA 6th edition
Turker, N.P., Bagci, U. & Bagci, P.O. (2019). Investigation of the Anticancer and Proliferative Effect of Broccoli Extract on Du145 Prostate Cancer and MEF Healthy Fibroblast Cell Lines . International Journal of Innovative Approaches in Agricultural Research, 3(4), 550-556. doi: 10.29329/ijiaar.2019.217.1

Harvard
Turker, N., Bagci, U. and Bagci, P. (2019). Investigation of the Anticancer and Proliferative Effect of Broccoli Extract on Du145 Prostate Cancer and MEF Healthy Fibroblast Cell Lines . International Journal of Innovative Approaches in Agricultural Research, 3(4), pp. 550-556.

Chicago 16th edition
Turker, Nebiye Pelin, Ufuk Bagci and Pelin Onsekizoglu Bagci (2019). "Investigation of the Anticancer and Proliferative Effect of Broccoli Extract on Du145 Prostate Cancer and MEF Healthy Fibroblast Cell Lines ". International Journal of Innovative Approaches in Agricultural Research 3 (4):550-556. doi:10.29329/ijiaar.2019.217.1.

References
  1. Canene-Adams, K.,  B. L. Lindshield, S. Wang, E. H. Jeffery, S. K. Clinton  and J. W. Erdman, Jr. (2007). Combinations of tomato and broccoli enhance antitumor activity in Dunning R3327-H prostate adenocarcinomas. Cancer Res.,  836-843.  [Google Scholar]
  2. Catalona, W.J. (2004). Management of the cancer of the prostate. New. Engl. J. Med., 331, 996-1004. [Google Scholar]
  3. Chan-Smutko, G., D. Patel, K. M. Shannon and P. D. Ryan (2008). Professional challenges in cancer genetic testing: who is the patient?.  Oncologist,  13(3), 232–238. [Google Scholar]
  4. Dawson, N.A. (1993). Treatment of progressive metastatic prostate cancer. Oncology, 7, 17-24. [Google Scholar]
  5. Hwang, J.-H. and S.-B. Lim (2015). Antioxidant and anticancer activities of broccoli by- products from different cultivars and maturity stages at harvest. Prev. Nutr. Food  Sci., 20(1):8-14. [Google Scholar]
  6. Kristen, M. S. and P. Devanshi (2010). Principles of Cancer Genetic Counseling and Genetic Testing. Handbook from the Massachusetts General Hospital, pp. 23-40.  [Google Scholar]
  7. Laufer, M., S. R. Denmeade, V. J. Sinibaldi, M. A. Carducci, M. A. Eisenberger (2000). Complete androgen  blockade for prostate cancer. What went wrong? J. Urol., 164, 3-9. [Google Scholar]
  8. Lynn, A.G. and M.S. Reis (1995). Top 5 cancers for females and males in the U.S. J. Natl. Cancer Inst., 87, 867-868. [Google Scholar]
  9. Murphy, G., K. Griffths, L. Denis, S. Khoury, C. Chatelain, A.T.K. Cockett (1997). Epidemiology and natural history of prostate cancer. Proc. 1st  International  Consultation on Prostate Cancer. Paris,  Manchecourt Scientific Communication Int., pp. 1-30. [Google Scholar]
  10. Rose, P.,  Q. Huang, C. N. Ong and M. Whiteman (2005). Broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells. Toxicol. Appl. Pharm., 209, 105 – 113.   [Google Scholar]