The present study aims to establish the application of fluorescence spectroscopy as a field method in the determination of varietal differences after carrot harvesting. The experimental studies were conducted on site at the farm where the carrot accessions were grown. The fluorescence analysis was carried out with a source with an emission wavelength of 285 nm and an author-developed mobile fiber-optic experimental set-up. The subjects of this research are root crops from Nantes, Short`n Sweet, Touchon, and Flakkee.

The correlation between the emission signals of the samples was established. This fact allows mobile fluorescence spectroscopy to be successfully applied as a rapid tool to establish the origin of unknown carrot accessions in the presence of a rich library of spectra as an applied tool in breeding programs. The results of the experiment can be used to optimize the time for the analysis of the varietal differences of the carrot genotypes after harvest. Fluorescence spectroscopy in a fiber-optical configuration will support the process of determining the belonging of a specific variety to a given variety (even for samples of unknown origin when it is necessary to qualify the result of accessions in a short time).