Detection and the Prevalence of Prune Dwarf Virus (PDV) in Important Cherry Plantations of the Eastern Mediterranean Region by DAS-ELISA and RT-PCR Analyzes

Peli̇n Gündoğan, Mona Gazel & Kadri̇ye Çağlayan

doi:https://doi.org/10.29329/ijiaar.2022.451.3

Original article | Open Access
International Journal of Innovative Approaches in Agricultural Research 2022, Vol. 6(2) 90-102

Detection and the Prevalence of Prune Dwarf Virus (PDV) in Important Cherry Plantations of the Eastern Mediterranean Region by DAS-ELISA and RT-PCR Analyzes

Peli̇n Gündoğan, Mona Gazel & Kadri̇ye Çağlayan

pp. 90 - 102 | DOI: https://doi.org/10.29329/ijiaar.2022.451.3

Published online: June 30, 2022 | Number of Views: 213 | Number of Download: 425

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Abstract

In this study, the prevalence of PDV, which infects cherry (Prunus avium L.) trees and is among the important viral pathogens in Adana, Kahramanmaraş and Osmaniye provinces where cherry is extensively produced in the Eastern Mediterranean region of Turkey, was investigated. A total of 173 cherry leaf samples with and without symptoms collected from Adana, Kahramanmaraş and Osmaniye provinces were tested by DAS-ELISA and RT-PCR methods. PDV was not detected in all samples tested by the DAS-ELISA method. As a result of the tests performed with RT-PCR, 874 bp long nucleotide sequences belonging to the coat protein gene region of the 15 samples were obtained and found positive. After the sequences were obtained, they were compared with the sequences from Turkey and the sequences of PDV reference isolates registered in GenBank. As a result of BLASTn analysis, it was determined that the Turkish PDV cherry isolates showed similarity between 89.58-99.32% at the nucleotide level with the PDV reference sequence. Phylogenetic analysis of the nucleotide sequences of the obtained isolates was performed and their relationship with PDV cherry isolates in the world and Turkey was determined.

Keywords: Prunus avium L., cherry, DAS-ELISA, RT-PCR, PDV, Sequence analysis

How to Cite this Article

APA 6th edition
Gundogan, P., Gazel, M. & Caglayan, K. (2022). Detection and the Prevalence of Prune Dwarf Virus (PDV) in Important Cherry Plantations of the Eastern Mediterranean Region by DAS-ELISA and RT-PCR Analyzes . International Journal of Innovative Approaches in Agricultural Research, 6(2), 90-102. doi: 10.29329/ijiaar.2022.451.3

Harvard
Gundogan, P., Gazel, M. and Caglayan, K. (2022). Detection and the Prevalence of Prune Dwarf Virus (PDV) in Important Cherry Plantations of the Eastern Mediterranean Region by DAS-ELISA and RT-PCR Analyzes . International Journal of Innovative Approaches in Agricultural Research, 6(2), pp. 90-102.

Chicago 16th edition
Gundogan, Peli̇n, Mona Gazel and Kadri̇ye Caglayan (2022). "Detection and the Prevalence of Prune Dwarf Virus (PDV) in Important Cherry Plantations of the Eastern Mediterranean Region by DAS-ELISA and RT-PCR Analyzes ". International Journal of Innovative Approaches in Agricultural Research 6 (2):90-102. doi:10.29329/ijiaar.2022.451.3.

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