International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article    |    Open Access
International Journal of Innovative Approaches in Agricultural Research 2022, Vol. 6(2) 112-128

Comparative Expression Profiles of SUVH7 in Sexual and Apomict Boechera spp. Display Differential Expression

Aslıhan Özbi̇len & Kemal Melih Taşkın

pp. 112 - 128   |  DOI: https://doi.org/10.29329/ijiaar.2022.451.5

Published online: June 30, 2022  |   Number of Views: 108  |  Number of Download: 234

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Abstract

Genomic imprinting is parent-of-origin specific gene expression in embryo nourishing tissues endosperm and placenta in flowering plants and mammals, respectively. Seeds are formed with double fertilization in flowering plants and the endosperm has a 3n chromosome set with the contribution of 2 maternal and 1 paternal genome. Any deviation from this ratio (2m+1p) results in seed abortion in many species, however, apomict species modify their gametogenesis or fertilization to survive. Boechera divaricarpa is a diploid apomict plant species that can produce seeds with a 4m:1p parental genome ratio in endosperm and produce viable seeds. SUVH7, on the other hand, is a histone methyltransferase that has a catalytic SET domain responsible for epigenetic control of gene expression. In this study, we characterized the structures of the SUVH7 gene and compared the mRNA levels of SUVH7 in diploid apomict and sexual Boechera spp. in unopened immature buds and manually pollinated siliques representing the -pre and -post pollination stages, respectively. The expression level of SUVH7 in apomict B. divaricarpa has reached the max level 48 hours later following pollination, while in sexual B. stricta its expression level has dramatically decreased. Therefore, our study suggests the importance of epigenetic reprogramming in apomicts during seed development since chromatin marks via SUVH7 are commonly associated with the activation of transcription in plants.

Keywords: SUVH7, Boechera divaricarpa, Boechera stricta, Apomixis, Seed development, qPCR

How to Cite this Article

APA 6th edition
Ozbi̇len, A. & Taskin, K.M. (2022). Comparative Expression Profiles of SUVH7 in Sexual and Apomict Boechera spp. Display Differential Expression . International Journal of Innovative Approaches in Agricultural Research, 6(2), 112-128. doi: 10.29329/ijiaar.2022.451.5

Harvard
Ozbi̇len, A. and Taskin, K. (2022). Comparative Expression Profiles of SUVH7 in Sexual and Apomict Boechera spp. Display Differential Expression . International Journal of Innovative Approaches in Agricultural Research, 6(2), pp. 112-128.

Chicago 16th edition
Ozbi̇len, Aslihan and Kemal Melih Taskin (2022). "Comparative Expression Profiles of SUVH7 in Sexual and Apomict Boechera spp. Display Differential Expression ". International Journal of Innovative Approaches in Agricultural Research 6 (2):112-128. doi:10.29329/ijiaar.2022.451.5.
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