International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article    |    Open Access
International Journal of Innovative Approaches in Agricultural Research 2019, Vol. 3(4) 557-565

Effect of Quinoa Plant on Metastasis and Ion Channels of Rat Brain Cancer Glioma Cell Lines

Nebiye Pelin Türker & Ufuk Bağcı

pp. 557 - 565   |  DOI: https://doi.org/10.29329/ijiaar.2019.217.2

Published online: December 10, 2019  |   Number of Views: 156  |  Number of Download: 734


Abstract

In this study, the effects of the quinoa plant on the rat brain cancer glioma cell line were examined. Rat C6 glioma cell lines were cultivated in Dulbecco's Minimum Essential Medium (DMEM) appendage with HAMS F 12 (1: 1) and 2% FBS. After proliferation, Quinoa plant was added into the cells and incubated at 37°C for 24 and 48 h in 5% CO2. The viability of the cells was identified by using the MTT method (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide). IC50 concentration was determined using the statistic software SPSS (Probit analysis). The effect of the quinoa plant on the invasiveness of the C6 cells was analyzed by the wound test and the changes in the ion concentrations in the cells were determined with ICP-MS. As a result of the MTT test, the IC50 value of the quinoa plant was determined as 50 ppb. Wound test showed that use of quinoa plant (50 ppb) inhibited metastasis in the glioma cells while the cell proliferation in the control group was continued. Furthermore, calcium, sodium and potassium ions, which are regulators of cell cycle, were found in higher concentrations in that the untreated control cells than quinoa treated cells. As a result of this study; ICP-MS analysis showed that higher levels of calcium, sodium, and potassium ions were found in the untreated cells, whereas the application of the quinoa plant decreased these values. This change in ion channels was thought to be associated with the invasion of glioma cells, and it was determined that quinoa had significant anticancer effects.

Keywords: quinoa, cancer, invasion, glioma, ion channels


How to Cite this Article

APA 6th edition
Turker, N.P. & Bagci, U. (2019). Effect of Quinoa Plant on Metastasis and Ion Channels of Rat Brain Cancer Glioma Cell Lines . International Journal of Innovative Approaches in Agricultural Research, 3(4), 557-565. doi: 10.29329/ijiaar.2019.217.2

Harvard
Turker, N. and Bagci, U. (2019). Effect of Quinoa Plant on Metastasis and Ion Channels of Rat Brain Cancer Glioma Cell Lines . International Journal of Innovative Approaches in Agricultural Research, 3(4), pp. 557-565.

Chicago 16th edition
Turker, Nebiye Pelin and Ufuk Bagci (2019). "Effect of Quinoa Plant on Metastasis and Ion Channels of Rat Brain Cancer Glioma Cell Lines ". International Journal of Innovative Approaches in Agricultural Research 3 (4):557-565. doi:10.29329/ijiaar.2019.217.2.

References
  1. Bennett, J.S., D.M. Stroud, J.R. Becker, D.M. Roden (2013). Proliferation of embryonic cardiomyocytes in zebrafish requires the sodium channel scn5Lab. Genesis, 51, 562–574. [Google Scholar]
  2. Campbell, T.M., M.J. Main and E.M. Fitzgerald (2013). Functional expression of the voltage-gated Na(+)-channel Nav1.7 is necessary for EGF-mediated invasion in human non-small cell lung cancer cells. J. Cell Sci., 126, 4939–4949. [Google Scholar]
  3. Catterall, W.A. (2012). Voltage-gated sodium channels at 60: structure, function and pathophysiology. J. Physiol., 590, 2577–2589. [Google Scholar]
  4. Chopra, S.S., D.M. Stroud, H. Watanabe, J.S. Bennett, C.G. Burns, K.S. Wells, T. Yang, T.P. Zhong and D.M. Roden (2010). Voltage-gated sodium channels are required for heart development in zebrafish. Circ. Res., 106, 1342–1350. [Google Scholar]
  5. Erdogan, M.A. and O. Erbas (2018), Ion channels and cancer. Fng J. Med. Sci., 49-62. [Google Scholar]
  6. Fraser, S.P., J. K. Diss, A.M. Chioni, M. E. Mycielska, Pan H, Yamaci RF, et al. (2005). Voltage-gated sodium channel expression and potentiation of human breast cancer metastasis. Clin. Cancer Res., 11, 5381-9. [Google Scholar]
  7. Han, X., Wang F, Yao W, Xing H, Weng D, Song X, et al. (2007). Heat shock proteins and p53 play a critical role in K+ channel-mediated tumor cell proliferation and apoptosis. Apoptosis, 12, 1837-1846. [Google Scholar]
  8. Hara, Y., Wakamori M, Ishii M, Maeno E, Nishida M, Yoshida T, et al. (2002).  LTRPC2 Ca+2-permeable channel activated by changes in redox status confers susceptibility to cell death. Mol. Cell, 9, 163-173. [Google Scholar]
  9. Hu, J., X. Yuan, M.K.Ko, D. Yin, M. R. Sacapano, X. Wang, et al. (2007) Calcium-activated potassium channels mediated blood-brain tumor barrier opening in a rat metastatic brain tumor model. Mol. Cancer, 6, 22. [Google Scholar]
  10. Kis-Toth, K., P. Hajdu, I. Bacskai, O. Szilagyi, F. Papp, A. Szanto, E. Posta, P. Gogolak, G. Panyi and E. Rajnavolgyi (2011). Voltage-gated sodium channel Nav1.7 maintains the membrane potential and regulates the activation and chemokine-induced migration of a monocyte-derived dendritic cell subset. J. Immunol., 187, 1273–1280. [Google Scholar]
  11. Lin, W.H.,  C. Gunay, R. Marley, A.A. Prinz and R.A. Baines (2012). Activity-dependent alternative splicing increases persistent sodium current and promotes seizure, J. Neurosci., 32, 7267–7277. [Google Scholar]
  12. Lu, F., H. Chen, C. Zhou, S. Liu, M. Guo, P. Chen, H. Zhang, D. Xie and S. Wu (2008).  T-type Ca2+ channel expression in human esophageal carcinomas: a functional role in proliferation. Cell Calcium, 43, 49-58. [Google Scholar]
  13. Marx, A., J. Siara and R. Rüdel (1991). Sodium and potassium channels in epithelial cells from thymus glands and thymomas of myasthenia gravis patients. Pflugers Arch., 417, 537-9. [Google Scholar]
  14. Mycielska, M.E., S. P. Fraser, M. Szatkowski and M. B. Djamgoz (2003). Contribution of functional voltage-gated Na+ channel expression to cell behaviors involved in the metastatic cascade in rat prostate cancer: II. Secretory membrane activity. J. Cell Physiol.,195, 461-9. [Google Scholar]
  15. Pancrazio, J. J., M. P. Viglione, I. A. Tabbara and Y. I. Kim (1989). Voltage-dependent ıon channels in small-cell lung cancer cells. Cancer Res., 49, 5901-5906. [Google Scholar]
  16. Potier, M., V. Joulin, S. Roger, P. Besson, M. L. Jourdan, J. Y. Le Guennec, P. Bougnoux and C. Vandier (2006). Identification of SK3 channel as a new mediator of breast cancer cell migration. Mol. Cancer Res., 5, 2946-2953. [Google Scholar]
  17. Roger, M., M. Potier, C. Vandier, P. Besson and J. Y. Le Guennec (2006). Voltage-gated sodium channels: new targets in cancer therapy? Curr. Pharm. Des., 12, 3681-3695. [Google Scholar]
  18. Yildirim, S., (2013). Expression of voltage-gated sodium channel in primary tumors and corresponding metastases in the Copenhagen rat model of prostate cancer. Journal of Clinical and Experimental Investigations, 422-428. [Google Scholar]