International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article    |    Open Access
International Journal of Innovative Approaches in Agricultural Research 2019, Vol. 3(2) 229-246

Occurrence and Biological Control of Cucumber Damping Off Disease under Protected Cultivation in Sulaimani, Iraq

Emad Al-Maaroof & Nermin M. Saber

pp. 229 - 246   |  DOI: https://doi.org/10.29329/ijiaar.2019.194.9

Published online: June 30, 2019  |   Number of Views: 173  |  Number of Download: 883


Abstract

Occurrence and cucumber damping-off disease incidence was determined in Sulaimani plastic houses in 2014 revealed from overall disease incidence of 6.82%. The highest incidence and severity reached to 23.7% and 5.0 respectively in Kharajian. While the lowest incidence and severity was detected in Arabit (0.2% and 0.6 respectively). Disease symptoms include pre and post-emergency damping-off of cucumber seedlings. Twelve fungal pathogens were isolated from roots and crown of infected seedlings and plants that explore typical damping-off and root rot symptoms. Rhizoctonia solani was the most frequently isolated fungi followed by Pythium aphanidermatum, Fusarium solani and Pythium sp. Morphology and characteristics of R. solani and P. aphanidermatum match with the original described characters of the fungi. The optimum growth temperature for P. aphanidermatum was 30°C and for R. solani was between 25-30°C. Pathogenicity test revealed that R. solani significantly surpassed all other treatment except P. aphanidermatum by inciting 53.3% pre and 66.4% post-emergency damping-off followed by P. aphanidermatum that incited 43.6% and 56.3% pre and post emergency damping-off respectively. T. harzianum showed high antagonistic ability against both pathogens. Antagonistic ability degree of T. harzianum reached to 37.02  against P. aphanidermatum and 32.00 against R. solani. The bio-control bacterial Bacillus subtilis, Rhizobacteria, Streptomyces coelicolor showed high efficiency in controlling the disease. Rhizobacteria and S. coelicolor completely inhibit R. solani growth at 10-1 bacteria dilution and significantly surpassed all other treatments. dilution 10-1 from all the used bacteria were significantly more efficient against P. aphanidermatum. This dilution was contain 21.4 × 107 cell forming unit in each milliliter (CFU/ml) in B. subtilis, 28 × 107(CFU/ml) in Rhizobacteria, 29.5 × 107 (CFU/ml) in S. coelicolor, 32.2× 107(CFU/ml) in Pseudomonas flouresence and 22.6 × 107 (CFU/ml) in Azotobacter chroococcus. 

Keywords: Cucumis sativus, biological control, fungal diseases, Iraq


How to Cite this Article

APA 6th edition
Al-Maaroof, E. & Saber, N.M. (2019). Occurrence and Biological Control of Cucumber Damping Off Disease under Protected Cultivation in Sulaimani, Iraq . International Journal of Innovative Approaches in Agricultural Research, 3(2), 229-246. doi: 10.29329/ijiaar.2019.194.9

Harvard
Al-Maaroof, E. and Saber, N. (2019). Occurrence and Biological Control of Cucumber Damping Off Disease under Protected Cultivation in Sulaimani, Iraq . International Journal of Innovative Approaches in Agricultural Research, 3(2), pp. 229-246.

Chicago 16th edition
Al-Maaroof, Emad and Nermin M. Saber (2019). "Occurrence and Biological Control of Cucumber Damping Off Disease under Protected Cultivation in Sulaimani, Iraq ". International Journal of Innovative Approaches in Agricultural Research 3 (2):229-246. doi:10.29329/ijiaar.2019.194.9.

References
  1. Agrios, G. N. (1997) Plant pathology. 4th Ed.  Academic press, New York U.S.A. pp 606. [Google Scholar]
  2. Aziz, A. Y., H. A. Foster and C. P. Fairhurst (1993) In vitro interactions between Trichoderma spp. and  Ophiostoma ulmi and their cat ions for the biological control of Dutch elm disease. And other fungal disease. Arb-bio-cultural Journal, 17 (2), 145-157. [Google Scholar]
  3. Bakker, P. A. H. M., L. X. Ran, C. M. J. Pieterse and L. C. Van Loon (2003). Understanding the involvement of rhizobacteria - mediated induction of systemic resistance in bio-control of  plant disease. Can. J. Plant Pathol., 25, 5-9. [Google Scholar]
  4. Barnett, H. L. and B. H. Hunter (1998). Illustrated Genera of Imperfect Fungi. Fourth edition. 241 pp. [Google Scholar]
  5. Beagle-Ristaino, J. E. and G. C. Papavizas (1985). Survival and proliferation of propagules of Trichoderma spp. and Gliocladium virens in soil and in plant rhizospheres. Phytopathology, 75, 729-732. [Google Scholar]
  6. Beattie, G.A. (2006). Plant-associated bacteria: survey, molecular phylogeny, genomics and recent advances. In: S.S. Gnanamanickam (Ed).Plant-Associated Bacteria. Springer, Netherlands, pp: 1-56. [Google Scholar]
  7. Ben-yephet, Y. and E. B. Nelson (1999). Differential suppression of damping-off caused by pythium aphanidermatum, p.irregular, and P. myriotylum in   composts   at    different    temperatures.  Plant Disease, 83, 356-360. [Google Scholar]
  8.  Bertagnolli, B. L., F. K. D. Soglio and J. B. Sinclair (1996). Extracellular enzyme profiles of the fungal pathogen Rhizoctonia solani isolate 2B) 12 and of two antagonists, Bacillus megaterium strain B153-2-2 and Trichoderma harzianum isolate Th008. I. Possible correlations with inhibition of growth and bio-control. Physiol. Mol. Plant. Path., 48, 145-160.  [Google Scholar]
  9. Cole, J. S. and Z. Zvenyika (1988). Integrated control of Rhizoctonia solani and Fusarium solani in tobacco transplants with Trichoderma harzianum and triadimenol. Plant Pathol., 37, 271-277. [Google Scholar]
  10. Devaki, N. S., S.S. Bhat, S. G. Bhat and K. R. Manjunatha (1992). Antagonistic activities of Trichoderma harzianum against Phythium aphanidermatum and Pythium myriotylum on tobacco. J. Phytopathol., 136, 82-87. [Google Scholar]
  11. Domsch, K. H., W. Gams and T. Anbreson (2007).Compendium of soil fungi. 2nd Ed. IHW-Verlag. Echoing. Germany, pp. 270-276.  [Google Scholar]
  12. Dowling D.N. and F. O. O’Gara (1994). Metabolites of Pseudomonas involved in the bio-control of plant disease. Trends Biotech., 12, 133-141. [Google Scholar]
  13. El-Behadli, A. H. and A. Al-Azawi (1979). Preliminary survey of disease greenhouse in Iraq. Iraqi J. Agric. Sci., 14, 15-38. [Google Scholar]
  14. F.A.O. 2001. Food and Agriculture organization of United Nations. Book Rome, Italy. [Google Scholar]
  15. Gravel, V., H. Antoun and R. J. Tweddell (2007). Effect of indole-acetic acid (IAA) on the development of symptoms caused by Pythium ultimum on tomato plants. Eur. J. Plant Pathol., 119, 457-462. [Google Scholar]
  16. Harman, G. E. (2000). Myths and Dogmas of Biological Changes are perceptions derived from research on Trichoderma  harzianum strain T.22. Plant Dis. Report., 84, 377-393 [Google Scholar]
  17. Hassan, Ahmed Abdel Moneim (1991). Production of vegetable crops. Arab House Baghdad University.  [Google Scholar]
  18. Hultberg, M., B. Alsanius and P. Sundin (2000). In vivo and in vitro interactions between Pseudomonas fluorescens and Pythium ultimum in the suppression of damping-off in tomato seedlings. Biological Control, 110, 19-18. [Google Scholar]
  19. Kwok, O. C. H., P.C.  Fahy, H.A.J. Hoitink and G. A. Kuter (1987). Interaction between bacteria and Trichoderma hamatum in suppression of Rhizoctonia damping-off in bark compost media. Phytopathology, 77(80), 1206-1212 [Google Scholar]
  20. Limon, M. C., I.A. Pintor – Toro and T. Benitez (1999). Increased antifungal activity of Trichoderma harzianum transformants that over express a33-kola chitinase. Physiopathology, 89, 254-261. [Google Scholar]
  21. Maksimov, I. V., R.R. Abizgil’dina and L. I. Pusenkova (2011). Plant growth promoting rhizobacteria as alternative to chemical crop protectors from pathogens (Review). Appl. Biochem. Microbiol., 47, 333-345. [Google Scholar]
  22. Mathre, D. F., R. J. Cook and N. W. Callan (1999). From discovery use: Trarersing the world of commercializing bio-control agent for plant disease control. Plant Disease, 83, 972-983. [Google Scholar]
  23. Mavrodi, O.V., B.B. Mespadden - Gardener, L.S. Thomashow, D.V. Mavrodi, R.F. Bonsall and D.M.Weller (2001). Genetic diversity of PhLD from 2, 4-diacetyl phloroglucinol producing flurescent Pseudomonas spp. Phytopathology, 91, 35-43. [Google Scholar]
  24. Neeraja, C., K. Anil, P. Purushotham, K. Suma, P. Sarma, B. M. Moerschbacher, and A. R. Podile (2010). Biotechnological approaches to develop bacterial chitinases as a bioshield against fungal diseases of plants. Crit. Rev. Bio-technol., 30, 231-241 [Google Scholar]
  25. Ongena, M., F. Daayf, P. Jacques, P. Thonart, N. Benhamou, T. C. Paulitz, P. Cornelis, N. Koedam and R.R. Belanger (1999). Protection of Cucumber against Pythium root rot by fluorescent Pseudomonads: Predominant role of induced resistance over siderophores and antibiosis. Plant Pathology, 48, 66-76. [Google Scholar]
  26. Ownley, B. H., B. K. Duffy and D. M. Weller (2003). Identification and manipulation of soil properties to improve the biological control performance of phenazineproducing Pseudomonas fluorescens. Appl. Environ. Microbiol., 69, 3333-3343. [Google Scholar]
  27. Punja, Z.  K. and R. Yip (2003). Biological control of damping-off and root rot caused by Phythium aphanidermatum on greenhouse Cucumbers. Can. J. Plant Pathol., 25, 411-417. [Google Scholar]
  28. Shameem, S. (2006). Studies of microbial antagonists showing antifungal activity against phytopathogenic fungi. Phd dissertation. pp 47-68. [Google Scholar]
  29. Shew, H. D. (1991). Pythium Diseases Compendium of Tobacco Diseases. G. Lucas and H. D. eds. American Phytopathological Society. St. Paul. MN. pp. 20-21. [Google Scholar]
  30. Sivan, A. and I. Chet (1989). The possible role competition between Trichoderma harzianum and Rhizoctonia oxysporum on Rhizosphere colonization. Phytopathology, 79 (2), 198-203. [Google Scholar]
  31. Stanghellini, M. E. and J. M. Phillips (1975). Pythium aphanidermatum: It’s Occurrence and Control with Pyroxychlor in the Arabian Desert at Abu-DhaBi. Plant Disease Reporter,  59, 559-563. [Google Scholar]
  32. Staplers, J. A. and T. E. Anderson (1996). Asynopsis of the taxonomy of teleomorphs connected with Rhizoctonia S. L. Rhizoctonia species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease control. B.senh, S. Jabaji-Hare, S. Neate and G. Dijst. (Eds). pp 49-63. [Google Scholar]
  33. Suslow, T.V. (1982). Role of root colonizing bacteria in plant growth. In Mount M.S. and G.H. Lacy, (EDS). Phytopathology, 5(1), 187-223 [Google Scholar]
  34. Uzuhashi, S., M. Tojo and M. Kakishima (2010). Phylogeny of the genus Pythium and description of new genera. Mycoscience, 51, 337-365. [Google Scholar]
  35. VanLoon, L. C. (1997). Induced resistance in plant and the role of Pathogenesis – related proteins. Eur. J. Plant Pathol., 103, 753-765. [Google Scholar]
  36. VanLoon, L. C. (2007). Plant responses to plant growth-promoting rhizobacteria. Eur. J. Plant Pathol., 119, 243-254. [Google Scholar]
  37. Watanabe, T. (1984). Detection and Quantitative Estimations of Pythium aphanidermatum in soil with Cucumber seeds as a baiting substrate. Plant Disease, 68, 697-698. [Google Scholar]
  38. Waterhouse, G. M. (1967). Key to Pythium Pringsheim. Mycol. Papers Commonwealth Mycol. Inst. Kew, Surrey, England, 15, 109. [Google Scholar]
  39. Yedidia, I. N. Benhamou and I. Chet (1998). Induction of defense responses in Cucumber plants (Cucumis sativus L.) by bio-control agent Trichoderma harzianum. Appl. Environ. Microbiol., 88, 1061-1070. [Google Scholar]
  40. Zhou, T. and T. C. Paulitz (1993). In vitro and in vivo effects of Pseudomonas spp. on Pythium aphanidermatum zoospore behavior in exudates and on the rhizoplane of bacteria - treated Cucumber roots. Physiopathology, 83, 872-876. [Google Scholar]
  41.                  الخفاجي ، هادي مهدي عبود. (1985). دراسة بايولوجية ووقائية للفطر Pythium aphanidermatum المسبب المرضي لسقوط بادرات الخيار في البيوت الزجاجية والبلاستيكية. رسالة ماجستبر. كلية الزراعة . جامعة بغداد.  [Google Scholar]
  42.                  الدليمي ، اسماعيل عباس جديع. (2000). تقويم كفاءة البكتريا Pseudomonas fluorescens Migula في استحثاث مقاومة جهازية في نبات الخيار ضد الفطرين Pythium aphanidermatum (Edson) Fitz و Pseudoperonospora cubensis (Berk and Curt) Rostow. اطروحة دكتوراه . كلية الزراعة . جامعة بغداد.                    [Google Scholar]
  43.                  القرغولي ، جبار محسن جابر حسين (1999) . تأثير البكتريا Pseudomonas fluorescens والمعاملة بكبريتات الكالسيوم على مسببي مرض التعفن الطريErwinia carotovora var. Carotovora    ومرض التعفن الجاف Fusarium solani على درنات البطاطا في الحقل واثناء الخزن. اطروحة دكتوراه. كلية الزراعة . جامعة بغداد.  [Google Scholar]
  44.                   المعروف ، عماد محمود غالب. (1984). تدهور اشجار الفاكهة ذات النواة الحجرية (المشمش ، الخوخ والاجاص) المتسبب بصورة رئيسية عن الفطر Phytophthora drechsleri Tucker. رسالة ماجستبر. كلية الزراعة . جامعة بغداد.  [Google Scholar]
  45.                   جبر ، كامل سلمان.  (1996). المقاومة الاحيائية للمعقد المرضي بين ديدان تعقد الجذور Meloidogyne javanica والفطر Rhizoctonia solani في الباذنجان. اطروحة دكتوراة . كلية الزراعة . جامعة بغداد. [Google Scholar]
  46.                    سعد ، نجاة عدنان . (2001). التداخل بين ديدان العقد الجذرية Meloidogyne javanica والفطر Rhizoctonia solani في الباذنجان ومقاومته احيائياً. رسالة ماجستير. كلية الزراعة، جامعة بغداد، العراق [Google Scholar]
  47.                    شريف ، فياض محمد وفضل عبدالحسين واحمد محمد عكاشة(1988 ). المقاومة الحيوية للفطر Pythium aphanidermatum (Edson) Fitz على الخيار في البيت البلاستيكي. مجلة البحوث الزراعية والموارد المائية. المجلد  (7) : 99-108. [Google Scholar]
  48. عبدالواحد ، اياد ومحمد عامر فياض وعلي سالم حسين الغالبي . (1996). تطبيق تقنية التلقيح البكتيري بالبكتريا Pseudomonas fluorescens على نبات الرز وتأثيرها على القدرة الانتاجية. مجلة اباء للابحث الزراعية. 6 (1): 71-83. [Google Scholar]
  49. علوان ، صباح لطيف  (2005) امكانية تصنيع مبيد إحيائي من الفطر Trichoderma harzianum Rifai لمكافحة مرض تعفن البذور وموت البادرات في الحنطة . اطروحة دكتوراه . كلية التربية للبنات . جامعة الكوفة العراق [Google Scholar]
  50. كمال الدين، زاهد نوري علي (2008).تأثير التداخل بين الفطر Trichoderma harzianum Rifai والفطر Aspergillus niger Van Tieghem في  حماية  نباتات الطماطة من الاصابة Fusarium oxysporum f.sp . lycopersici .رسالة ماجستير- كلية الزراعة – جامعة الكوفة. [Google Scholar]