International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Research article    |    Open Access
International Journal of Innovative Approaches in Agricultural Research 2025, Vol. 9(4) 301-315

Genetic Characterization and Brown Rust Resistance Screening of Gamma Irradiated Bread Wheat M4 Populations by DNA Markers

Deniz Aşkar, Behiye Banu Bilgen

pp. 301 - 315   |  DOI: https://doi.org/10.29329/ijiaar.2025.1375.3

Publish Date: December 17, 2025  |   Single/Total View: 0/0   |   Single/Total Download: 0/0

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Abstract

The rapid increase in the world population has increased the demand for wheat, and breeding studies that enable the development of high-yield varieties resistant to various stress conditions have accelerated to meet this demand. Brown rust disease poses a serious threat to sustainable wheat cultivation. Classical breeding studies are effectively used in variety development in important agricultural plant species, supported by mutation and molecular breeding studies. In recent years, retrotransposon-based molecular markers have been widely used to better understand the variation in genotypes obtained in breeding processes. In this study, 15 retrotransposon based molecular markers, iPBS primers, were used for molecular characterization in M4 bread wheat populations exposed to different doses of gamma radiation. In addition, the presence of 8 Lr genes was examined in order to determine resistance to brown rust disease, which is one of the important causes of yield loss in various wheat varieties. A total of 102 bands belonging to iPBS primers were observed in 35 genotypes belonging to the seven bread wheat varieties analyzed. The average polymorphism rate of the bread wheat populations used in the study was calculated as relatively low (5.88%). It was observed that the studied wheat varieties had six Lr gene regions (Lr13, Lr19, Lr22a, Lr24, Lr37, and Lr47) except for the Lr14a and Lr34 genes from brown rust resistance genes. The molecular data obtained from the study will provide significant information for using mutation breeding and molecular breeding in classical breeding studies in bread wheat.

Keywords: iPBS, Lr Genes, Molecular Markers, Retrotransposons, Triticum aestivum

How to Cite this Article?

APA 7th edition
Askar, D., & Bilgen, B.B. (2025). Genetic Characterization and Brown Rust Resistance Screening of Gamma Irradiated Bread Wheat M4 Populations by DNA Markers. International Journal of Innovative Approaches in Agricultural Research, 9(4), 301-315. https://doi.org/10.29329/ijiaar.2025.1375.3

Harvard
Askar, D. and Bilgen, B. (2025). Genetic Characterization and Brown Rust Resistance Screening of Gamma Irradiated Bread Wheat M4 Populations by DNA Markers. International Journal of Innovative Approaches in Agricultural Research, 9(4), pp. 301-315.

Chicago 16th edition
Askar, Deniz and Behiye Banu Bilgen (2025). "Genetic Characterization and Brown Rust Resistance Screening of Gamma Irradiated Bread Wheat M4 Populations by DNA Markers". International Journal of Innovative Approaches in Agricultural Research 9 (4):301-315. https://doi.org/10.29329/ijiaar.2025.1375.3
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