International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article | International Journal of Innovative Approaches in Agricultural Research 2019, Vol. 3(1) 123-131

Extraction and Antistaphylococcal Study of the Essential Oil of Origanum vulgare L. (Guelma-Algeria)

Karima Ounaissia, Nora Mahfouf, Salima Bennadja, Abdelghani Djahoudı & Hichem Nasrı

pp. 123 - 131   |  DOI:

Published online: March 29, 2019  |   Number of Views: 108  |  Number of Download: 377


Antimicrobial properties of plant essential oils (EO) have been investigated through several observations and clinical studies which purpose them as potential tools to overcome the microbial drug resistance (MDR) problem. The aim of this research was to study the antibacterial effect of a traditional plant EO, Oraginum vulgare L., against clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) through disk diffusion and agar dilution methods.

The EO showed very effective bactericidal activity towards the majority of the tested bacterial strains with inhibition zone diameters in the range of 9.9-31.9 mm and a minimum inhibitory concentration (MIC) ranging from 0.314 to 0.628 mg/ml.

These results suggest that the essential oil of Origanum vulgare L. may be a useful alternative to antibiotics for the control of the infections caused by Staphylococcus aureus.

Keywords: Origanum vulgare L., Essential oil, Antibacterial activity, Staphylococcus aureus MRSA

How to Cite this Article?

APA 6th edition
Ounaissia, K., Mahfouf, N., Bennadja, S., Djahoudi, A. & Nasri, H. (2019). Extraction and Antistaphylococcal Study of the Essential Oil of Origanum vulgare L. (Guelma-Algeria) . International Journal of Innovative Approaches in Agricultural Research, 3(1), 123-131. doi: 10.29329/ijiaar.2019.188.12

Ounaissia, K., Mahfouf, N., Bennadja, S., Djahoudi, A. and Nasri, H. (2019). Extraction and Antistaphylococcal Study of the Essential Oil of Origanum vulgare L. (Guelma-Algeria) . International Journal of Innovative Approaches in Agricultural Research, 3(1), pp. 123-131.

Chicago 16th edition
Ounaissia, Karima, Nora Mahfouf, Salima Bennadja, Abdelghani Djahoudi and Hichem Nasri (2019). "Extraction and Antistaphylococcal Study of the Essential Oil of Origanum vulgare L. (Guelma-Algeria) ". International Journal of Innovative Approaches in Agricultural Research 3 (1):123-131. doi:10.29329/ijiaar.2019.188.12.

  1. Adam, K., A. Sivropoulou, S. Kokkini, T. Lanaras and M. Arsenakis (1998). Antifungal activity of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi. J. Agric. Food Chem., 46, 1739–1745. [Google Scholar]
  2. Ahmad, V.U., A.R. Jassbi and M.S.C. Pannahi (1999). Analysis of the essential oil of Echinophora sibthorpiana Guss  by means of GC, GC/MS and 13C-NMR techniques. J. Essent. Oil Res., 11, 107-108. [Google Scholar]
  3. Barros, J.C., M. L. De Conceicão, N.J. Da Gomes Neto, C.V. Costa, J.P. Da Siqueira Júnior, I. D. Basílio Junior and E. L. De Souza (2009). Interference of Origanum vulgare L. essential oil on the growth and some physiological characteristics of Staphylococcus aureus strains isolated from foods. LWT—Food Sci. Technol. 42 (6), 1139–1143. [Google Scholar]
  4. Baydar, H., O. Sagdiç, G. Ozkan and T. Karadogan (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Sartureja species with commercial importance in Turkey. Food Cont., 15, 169-172. [Google Scholar]
  5. Boucher, H. W., G. H.Talbot, J. S. Bradley, J.E. Edwards, D. Gilbert, L.B.Rice, M.Scheld, B. Spellberg and J. Bartlett (2009). Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clin. Infect. Dise., 48 (1), 1–12. [Google Scholar]
  6. Bouhdid, S., J. Abrini, D. Baudoux, A. Manresa and A. Zhiri (2012). Les huiles essentielles de l’Origan compact et de la cannelle de Ceylan: pouvoir antibactérien et mécanisme d’action. J. Pharm. Clin., 31(3), 141-148.   [Google Scholar]
  7. Cohen, M. L. (1992). Epidemiology of drug resistance: implications for a postantimicrobialera. Science, 257, 1050-1055. [Google Scholar]
  8. DeLeo, F. R., M. Otto, B.N. Kreiswirth and H.N. Chambers (2010). Community-associated methicillin resistant Staphylococcus aureus. Lancet, 375, 1557–1568. [Google Scholar]
  9. Dorman, H. J. D. and S.G. Deans (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J. Appl. Microbiol., 88, 308–316. [Google Scholar]
  10. Ellof, J.N. (1998). Which extractant should be used for the screening and isolation of antimicrobial components from plants? J. Ethnopharmacol., 60, 1-6. [Google Scholar]
  11. Friedman, M., P.R. Henika and R. E. Mandrell (2002). Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. J. Food. Prot., 65, 1545–1560. [Google Scholar]
  12. Giamarellou, H. (2010). Multidrug-resistant Gram-negative bacteria: how to treat and for how long, Int. J. Antimicrob. Agents, 36, Suppl. 2, S50–S54. [Google Scholar]
  13. Helander, I. M., H. L. Alakomi, K. Latva-Kala, T. Mattila-Sandholm, I. Pol, E. J. Smid, L. G. M. Gorris and A. von Wright (1998). Characterization of the action of selected essential oil components on Gram-negative bacteria. J. Agric. Food Chem., 46, 3590–3595. [Google Scholar]
  14. Juven, B. J., J.  Kanner, F. Schved and H. Weisslowicz, H. (1994). Factors that interact with the antibacterial action of thyme essential oil and its active constituents. J. Appl. Bacteriol., 76, 626–631. [Google Scholar]
  15. Lambert, R. J. W., P. N. Skandamis, P. J. Coote and G. J. E. Nychas (2001). A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J. Appl. Microbiol., 91, 453–462. [Google Scholar]
  16. Li, M., B. M. Diep, A. Villaruz, K. Braughton, X. Jiang, F. Deleo, H. Chambers and O. Lu yuan (2009). Evolution of virulence in epidemic community-associated methicillin-resistant Staphylococcus aureus. Proc. Natl. Acad. Sci. USA. 106, 5883-5888. [Google Scholar]
  17. Lin, Y.T., R.G., Labbe and K. Shetty (2004). Inibition of Listeria monocytogenes in fish and meat system by use of oregano and cramberry phytochemical synergies. Appl. Environ. Microbiol., 70, 5672-5678. [Google Scholar]
  18. Mahfouf, N., S. Bennadja, H. Nasri, A. Djahoudi and S. Heni (2017). Antioxidant and antibacterial activities of essential oil of Origanum vulgare L. against Escherichia coli stains. J. Essent. Oil Bear. Pl., 20(5), 1391-1399.   [Google Scholar]
  19. Manohar, V., C. Ingram, J. Gray, N. A.  Talpur, B. W. Echard, D. Bagchi and G. Preuss  (2001). Antifungal activities of origanum oil against Candida albicans. Mol. Cell Biochem., 228, 111–117. [Google Scholar]
  20. Marino, M., C. Bersani and G. Comi (2001). Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. Int. J. Food Microbiol., 67, 187-195. [Google Scholar]
  21. National Committee for Clinical Laboratory Standard. (2000). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7 A5. National Committee for Clinical Laboratory Standard, Wayne. [Google Scholar]
  22. Nostro, A., A. R. Blanco, M. A. Canntelli, V. Enea, G. Flamini, I. Morelli, A. S. Roccaro and V. Alonzo (2004). Susceptibility of methicillin-resistant staphylococci to oregano essential oil, carvacrol and thymol. FEMS Microbiol. Let., 230, 191-195. [Google Scholar]
  23. Pozzo, M.D., E. S. Loreto, D. F. Santurio, S. H. Alves, L. Rossatto, A. C. De Vargas, J. Viegas and M. M. Costa (2012). Antibacterial activity of essential oil of cinnamon and trans-cinnamaldehyde against Staphylococcus spp. isolated from clinical mastitis of cattle and goats. Acta Sci. Vet., 40, 1080. [Google Scholar]
  24. Rafee, Y., N. Abdel-Haq, B. Asmar, T. Salimnia, C.V. Pharm, P.M.J. Rybak and M. Amjad, (2012). Increased prevalence of methicillin-resistant Staphylococcus aureus nasal colonization inbhousehold contacts of children with community acquired disease. BMC Infect. Dis. doi:10.1186/1471-2334-12-4. [Google Scholar] [Crossref] 
  25. Santos, P.R.V., A.C.X. Oliveira and T.C.B. Tomassini (1995). Controle microbiógico de produtos fitoterápicos. Rev. Farm. Bioquím. 31, 35-38. [Google Scholar]
  26. Shetty, K. and M.L.  Wahlqvist (2004). A model for the role of prolinelinked pentose phosphate pathway in phenolic phytochemicals biosynthesis and mechanism of action from human health and environmental applications. Asia Pac. J. Clin. Nutr., 13, 1-24. [Google Scholar]
  27. Skandamis, P., E. Tsigarida and G.J.E. Nichas (2002). The effect of oregano essential oil on survival/death of Salmonella typhimurium in meat stored at 5°C under aerobic, VP/MAP conditions. Food Microbiol., 19, 97-108. [Google Scholar]
  28. Souza, E. L., T. L. Stamford and E. O. Lima (2006). Sensitivity of spoiling and pathogen-food related bacteria to Origanum vulgare L. (Lamiaceae) essential oil. Braz. J. Microbiol., 37, 527-532. [Google Scholar]
  29. Souza, E. L., T. L. M. Stamford, E. O. Lima  and V. N. Trajano (2007). Effectiveness of Origanum vulgare L. essential oil to inhibit the growth of food spoiling yeasts. Food Cont., 18, 409-413. [Google Scholar]
  30. Trombetta, D., F. Castelli, M.G. Sarpietro, V. Venuti, M. Cristani, C. Daniele, A. Saija, G. Mozzanti and G. Bisignano (2005). Mechanisms of antibacterial action of three monoterpenes. Antimicrob. Agents Chemother., 49, 2474–2478. [Google Scholar]
  31. Ultee, A., L. G. Gorris and E. J. Smid (1998). Bactericidal activity of carvacrol towards the food-borne pathogen Bacillus cereus. J. Appl. Microbiol., 85, 211–218. [Google Scholar]
  32. Ultee, A., E. P. W. Kets and J. Smid (1999). Mechanisms of action of carvacrol on the food-borne pathogen Bacillus cereus. Appl. Environ. Microbiol., 65, 4606–4610. [Google Scholar]
  33. Wang, J.T., C.H. Liao, C.T. Fang, W.C. Chie, M.S. Lai, T. L. Lauderdale and S.C. Chang (2010). Incidence of and risk factors for community-associated methicillin-resistant Staphylococcus aureus acquired infection or colonization in intensive-care-unit patients. J. Clin. Microbiol., 48, 4439–4444. [Google Scholar]