International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Research article    |    Open Access
International Journal of Innovative Approaches in Agricultural Research 2025, Vol. 9(3) 232-245

Effect of Two Different Blueberry (Vaccinium Corymbosum L.) Juice Processing Methods on Polyphenolic and Anthocyanin Contents

Onur Ercan, Buket Aşkın, Erdoğan Küçüköner

pp. 232 - 245   |  DOI: https://doi.org/10.29329/ijiaar.2025.1356.6

Publish Date: October 01, 2025  |   Single/Total View: 0/0   |   Single/Total Download: 0/0

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Abstract

Blueberry juice has become a popular beverage due to its nutritional value, including vitamins, minerals, and antioxidants. This study examined the effects of pressing, mashing, enzymatic treatment, and pasteurization on the anthocyanin content, color, and polyphenolic composition of blueberry juice (BJ). Enzymatic treatment caused a significant reduction (30%) in anthocyanin (ACN) content in Direct Juice Extraction (DJE), whereas Mash Treatment Processing (MTP) led to a significant increase. Overall, ACN levels were higher in treated samples after each processing step in MTP, while polyphenolic levels showed a slight increase in both DJE and MTP. However, both ACNs and polyphenolics decreased after pasteurization. BJ is effective for processing fresh juice. Additionally, throughout the processing stages, ACN, phenolic content, and antioxidant activity were higher, and juice yield was greater in comparison to both methods.

Keywords: Anthocyanins, Blueberry, Depectinization, Polyphenols

How to Cite this Article?

APA 7th edition
Ercan, O., Askin, B., & Kucukoner, E. (2025). Effect of Two Different Blueberry (Vaccinium Corymbosum L.) Juice Processing Methods on Polyphenolic and Anthocyanin Contents. International Journal of Innovative Approaches in Agricultural Research, 9(3), 232-245. https://doi.org/10.29329/ijiaar.2025.1356.6

Harvard
Ercan, O., Askin, B. and Kucukoner, E. (2025). Effect of Two Different Blueberry (Vaccinium Corymbosum L.) Juice Processing Methods on Polyphenolic and Anthocyanin Contents. International Journal of Innovative Approaches in Agricultural Research, 9(3), pp. 232-245.

Chicago 16th edition
Ercan, Onur, Buket Askin and Erdogan Kucukoner (2025). "Effect of Two Different Blueberry (Vaccinium Corymbosum L.) Juice Processing Methods on Polyphenolic and Anthocyanin Contents". International Journal of Innovative Approaches in Agricultural Research 9 (3):232-245. https://doi.org/10.29329/ijiaar.2025.1356.6
References
  1. Askin, B., Türkyılmaz, M., Özkan, M. & Küçüköner, E. (2022). Changes in anthocyanins and colour of black mulberry (Morus nigra) juice during clarification and pasteurization. J Food Meas Charact. 16, 784-792. DOI: 10.1007/s11694-021-01198-w. [Google Scholar]
  2. Buchert, J., Koponen, J., Suutarinen, M. & Mustranta, A. (2005). Effect of enzyme-aided pressing on anthocyanin yield and profiles in bilberry and blackcurrant juices. J Sci Food Agric. 85(15), 2548-2556. [Google Scholar]
  3. Chang, T. S., Sıddıq, M., Sınha, N. K. & Cash, J. N. (1994). Plum juice quality affected by enzyme treatment and fining. J Food Sci. 59(5), 1065-1069. DOI: 10.1111/j.1365-2621.1994.tb08191.x. [Google Scholar]
  4. Chevalier, L. M., Rioux, L. E., Angers, P. & Turgeon, S. L. (2019). Low-Temperature Blanching as a Tool to Modulate the Structure of Pectin in Blueberry Purees. Food Chem. 82, 2070–2077. DOI: 10.1111/1750-3841.13826. [Google Scholar]
  5. Dereli, U., Türkyilmaz, M., Yemiş, O. & Özkan, M. (2015). Effects of Clarification and Pasteurization on the Phenolics, Antioxidant Capacity, Color Density and Polymeric Color of Black Carrot (Daucus Carota L.) Juice. J Food Biochem. 39(5), 528-537. [Google Scholar]
  6. Dobson, G., McDougall, G. J., Stewart, D., Cubero, M. A & Karjalainen, R. O. (2016). Effects of Juice Matrix and Pasteurization on Stability of Black Currant Anthocyanins during Storage. J Food Sci. 82, 44-52. DOI:10.1016/j.lwt.2018.10.032. [Google Scholar]
  7. Fernandes, A., Bras, N. F., Mateus, N. & de Freitas, V. (2014). Understanding the Molecular Mechanism of Anthocyanin Binding to Pectin. Langmuir. 30, 8516-8527. [Google Scholar]
  8. Giusti, M. M. & Wrolstad, R. E. (2005). Unit F1.2: Characterization and measurement of anthocyanins by UV–visible spectroscopy. In R. E. Wrolstad, T. E. Acree, E. A. Decker, M. H. Penner, D. S. Reid, S. J. Schwartz, et al. (Eds.), Handbook of food analytical chemistry (pp. 19–31). New York: John Wiley & Sons. [Google Scholar]
  9. Hager, T. J., Howard, L. R. & Prior, R. L. (2008). Processing and storage effects on monomeric anthocyanins, percent polymeric colour, and antioxidant capacity of processed blackberry products. J Agric Food Chem. 56, 689–695. [Google Scholar]
  10. Howard, L., Clark, J. R. & Brownmiller, C. (2003). Antioxidant capacity and phenolic content in blueberries as affected by genotype and growing season. J Sci Food Agric. 83, 1238-1247. DOI: 10.1002/jsfa.1532. [Google Scholar]
  11. Kader, F., Rovel, B., Girardin, M. & Metche, M. (1999). Mechanism of Browning in Fresh Highbush Blueberry Fruit (Vaccinium corymbosum L). Role of Blueberry Polyphenol Oxidase, Chlorogenic Acid and Anthocyanins. J Sci Food Agric. 74(1), 31-34 (1999). DOI:10.1002/(SICI)1097-0010(199705)74:13.0.CO;2-9. [Google Scholar]
  12. Kalt, W., Ryan, D. A., Duy, J. C., Prior, R. L., Ehlenfeldt, K. & Kloet, S. P. V. (2001). Interspecific variation in anthocyanins, phenolics, and antioxidant capacity among genotypes of highbush and lowbush blueberries (Vaccinium section cyanococcus spp.). J Agric Food Chem. 49, 4761-7. DOI: 10.1021/jf010653e. [Google Scholar]
  13. Koponen, J. M., Buchert, J., Poutanen, K. S. & Törrönen, A.R. (2008). Effect of pectinolytic juice production on the extractability and fate of bilberry and black currant anthocyanins. Eur Food Res Technol. 227, 485–494. [Google Scholar]
  14. Kulcan, A. A, Öziyci, H. R., Tetik, N. & Karhan, M. (2015). Changes in turbidity, total phenolic and anthocyanin contents of clear red grape juice during processing. Food. 40:(6), 311-317 (2015). DOI: 10.15237/gida.GD15029. [Google Scholar]
  15. Landbo, A. & Meyer, A. A. (2004). Effects of different enzymatic maceration treatments on enhancement of anthocyanins and other phenolics in black currant juice. Innov Food Sci Emerg Technol. 5, 503-513. DOI: 10.1016/J.IFSET.2004.08.003. [Google Scholar]
  16. Lee, J. & Wrolstad, R. E. (2004). Extraction of anthocyanins and polyphenolics from blueberry processing waste. J Food Sci. 69(1), 564–573. [Google Scholar]
  17. Mikkelsen, B. B. & Poll, L. (2002). Decomposition and transformation of aroma compounds and anthocyanins during black currant (Ribes nigrum L.) juice processing. J Food Sci. 67, 3447–3451. [Google Scholar]
  18. Moyer, R. A., Hummer, K. E., Finn, C. E., Frei, B. & Wrolstad, R.E. (2008). Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: Vaccinium, Rubus, and Ribes. J Agric Food Chem. 50, 519-525. [Google Scholar]
  19. Perreault, V., Gouin, N., Bérubé, A., Villeneuve, W., Pouliot, Y. & Doyen, A. (2021). Effect of Pectinolytic Enzyme Pretreatment on the Clarification of Cranberry Juice by Ultrafiltration. Membranes. 11(1), 55. DOI: 10.3390/membranes11010055. [Google Scholar]
  20. Rapeanu, G., Loey, A. V., Smout, C. & Hendrickx, M. (2005). Thermal and high-pressure inactivation kinetics of polyphenol oxidase in Victoria grape must. J Agric Food Chem. 53, 2988-2994. DOI: 10.1021/jf0482515. [Google Scholar]
  21. Rommel, A., Heatherbell, D. A. & Wrolstad, R. E. (1990). Red raspberry juice and wine: efect of processing and storage on anthocyanin pigment composition, colour and appearance. J Food Sci. 55, 1011–1017. [Google Scholar]
  22. Saeeduddin, M., Abid, M., Jabbar, S., Wu, T., Hashim, M. M., Awad, F. N., Hu, B., Lei, S. & Zeng, X. (2015). Quality assessment of pear juice under ultrasound and commercial pasteurization processing conditions. Lebensm Wiss Technol. 64, 452-458. [Google Scholar]
  23. Sadilova, E., Stintzing, F. C. & Carle, R. (2006). Thermaldegradation of acylated and nonacylated anthocyanins. J Food Sci. 71, 504 – 512. [Google Scholar]
  24. Siddiq, M. & Dolan, K. D. (2017). Characterization of polyphenol oxidase from blueberry (Vaccinium corymbosum L.). Food Chem. 218, 216-220. DOI: 10.1016/j.foodchem.2016.09.061. [Google Scholar]
  25. Skrede, G., Wrolstad, R. E. & Durst, R. W. (2000). Changes in anthocyanins and polyphenolics during juice processing of highbush blueberries (Vaccinium corymbosum L.). J Food Sci. 65, 357-364. [Google Scholar]
  26. Sulaimana, A., Soo, M. J., Filipa, M. F. & Silvaa, V. M. (2008). Thermosonication for polyphenoloxidase inactivation in fruits: Modeling the ultrasound and thermal kinetics in pear, apple, and strawberry purees at different temperatures. J Food Eng. 165, 133-140. [Google Scholar]
  27. Szajdek, A. & Borowska, E. J. (2008). Bioactive compounds and health-promoting properties of berry fruits: A review. Plant Foods Hum Nutr. 63, 147-156. DOI: 10.1007/s11130-008-0097-5. [Google Scholar]
  28. Terefe, N. S., Delon, A., Buckow, R. & Versteeg, C. (2015). Blueberry polyphenol oxidase: Characterization and the kinetics of thermal and high pressure activation and inactivation. Food Chem. 188, 193. DOI: 10.1016/j.foodchem.2015.04.040. [Google Scholar]
  29. Threlfall, R. T., Morris, J. R., Howard, L. R., Brownmiller, C. R. & Walker, T. L. (2006). Pressing Effects on Yield, Quality, and Nutraceutical Content of Juice, Seeds, and Skins from Black Beauty and Sunbelt Grapes. J Food Sci. 70, S167-S171. [Google Scholar]
  30. Türkyılmaz, M., Tağı, Ş., Dereli, U. & Özkan, M. (2013). Effects of various pressing programs and yields on the antioxidant activity, antimicrobial activity, phenolic content and colour of pomegranate juices. Food Chem. 138, 138. DOI: 10.1016/j.foodchem.2012.11.100. [Google Scholar]
  31. Türkyılmaz, M., Yemiş, O. & Özkan, M. (2012). Clarifcation and pasteurisation efects on monomeric anthocyanins and percent polymeric colour of black carrot (Daucus carota L.) juice. Food Chem. 134, 1052–1058. [Google Scholar]
  32. Turfan, Ö., Türkyilmaz, M., Yemis, Ö. & Özkan, M. (2011). Effects of clarification and storage on anthocyanins and color of pomegranate juice concentrates. J Food Qual. 35, 272–282. DOI: 10.1111/j.1745-4557.2012.00451.x [Google Scholar]
  33. Weber, F. & Larsen, L. R. (2017). Influence of fruit juice processing on anthocyanin stability. Food Res Int. 100, 354-365. [Google Scholar]
  34. Yilmaz, Y., Toledo, R. (2005). Antioxidant Activity of Water-Soluble Maillard Reaction Products. Food Chem. 93, 273-278 (2005). DOI:1016/j.foodchem.2004.09.043. [Google Scholar]
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