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International Journal of Innovative Approaches in Agricultural Research 2024, Vol 8(4) 289-299

The Effect of Different Nitrogen Forms on Tomato Spotted Wilt Virus Infection in Pepper Plants Grown in Full and Deficient-Water Conditions

Zeynep Aslan • Miray Arlı Sökmen • Coşkun Gülser
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Article Type
Research article
Publication Date
December 31, 2024
Pages
289-299
DOI
10.29329/ijiaar.2024.1109.2

Abstract

Abiotic stress factors have an impact on both plants and their pathogens. Water and nutrient deficiencies are among the major abiotic stress factors in agricultural systems. Viruses are obligate parasite pathogens causing detrimental yield reductions on crop plants worldwide. However, there have been limited studies on the impact of abiotic factors on plant-virus interactions. In this study, the effects of different forms of nitrogen (N) fertilizers on Tomato spotted wilt virus (TSWV) infection in pepper plants grown under two different irrigation regimes [water deficient (30%) and full irrigation] were investigated in a growth room condition. Fertilizer applications consisted of five individual treatments with three replications. The pepper plants were supplied with 12 kg of N/da using different sources of nitrogen [urea (CH4N2O), NH4NO3, Mg(NO3)2 and (NH4)2SO4]. MgSO4 was also added to the experiment for comparison of the effects of Mg2+ and SO42- in the fertilizers. Each treatment consisted of TSWV-inoculated plants, as well as non-inoculated (healthy) and non-fertilized plants as controls. Every plant in all treatments was tested by enzyme-linked immunosorbent assay (ELISA) to confirm virus infection four and eight weeks after mechanical inoculation. The results showed that the virus-inoculated plants treated with urea and NH4NO3 had lower ELISA absorbance values in both deficient and full-irrigated conditions (p<0.01), suggesting greater tolerance to TSWV infection with these fertilizers. Additionally, the plants treated with Mg(NO3)2-containing fertilizer were relatively more affected by the virus under full irrigation than in water-deficient conditions. Conversely, the plants treated with (NH4)2SO4 had high virus content in deficient irrigation conditions. Despite better canopy development in full-irrigated conditions, TSWV symptoms in pepper plants were less prominent in water-deficient conditions than in full irrigation across all nutrient treatments. This study suggests that urea, NH4NO3, and Mg(NO3)2 may provide a positive contribution to plant fitness and virus suppression under water-deficient conditions. It is necessary to conduct further research to determine the impact of different nutrients and water levels on plant-virus interactions in field conditions.

Keywords: TSWV Plant Nutrients Water Stress Disease Tolerance
Cite this article
Aslan, Z., Arlı Sökmen, M., & Gülser, C. (2024). The Effect of Different Nitrogen Forms on Tomato Spotted Wilt Virus Infection in Pepper Plants Grown in Full and Deficient-Water Conditions. International Journal of Innovative Approaches in Agricultural Research, 8(4), 289-299. https://doi.org/10.29329/ijiaar.2024.1109.2

  • Abudurexiti, A., Adkins, S., Alioto, D., Alkhovsky, S. V., Avšič-Županc, T., et al. (2019). Taxonomy of the order Bunyavirales. Arch. Vir., 164:1949-1965. https://doi.org/10.1007/s00705-019-04253-6. [Google Scholar] [Crossref] 
  • Agrios, G. (2005). Plant Pathology. 5th Edition, Elsevier Academic Press, Amsterdam. 922 pp. [Google Scholar]
  • Alazem, M., Lin, K.Y. and Lin, N.S. (2014). The abscisic acid pathway has multifaceted effects on the accumulation of bamboo mosaic virus. Molecular Plant-Microbe Interact, 27: 177-189. http://dx.doi.org/10.1094/MPMI-08-13-0216-R. [Google Scholar]
  • Alazem, M, Lin, N. S. (2015). Roles of plant hormones in the regulation of host–virus interactions. Molecular Plant Pathology. 16 (5): 529-540. doi: 10.1111/mpp.12204. [Google Scholar] [Crossref] 
  • Bolat, İ. ve Kara, Ö. (2017). Bitki besin elementleri: Kaynakları, işlevleri, eksik ve fazlalıkları. Bartın Orman Fakültesi Dergisi, 19(1): 218-228. Doi: 10.24011/barofd.251313. [Google Scholar]
  • Bolton, M. D. and Thomma, B. P. H. J. (2008). The complexity of nitrogen metabolism and nitrogen-regulated gene expression in plant pathogenic fungi. Physiol. Mol. Plant Path., 72: 104-110. https://doi.org/10.1016/j.pmpp.2008.07.001. [Google Scholar] [Crossref] 
  • Camele, I., Candido, V., Miccolis, V. and Rana, G. L. (2000). Influence of nitrogen fertilization and water stress on incidence of virus infections in sweet pepper culture under glasshouse. Atti, Giornate Fitopatologiche, Perugia, Vol. Primo: 51-54. [Google Scholar]
  • Clark, M.F. and Adams, A.N. (1977). Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol., 34(3): 475-483. [Google Scholar]
  • Damayanti, T. A., Putra, L. K. (2011). First occurrence of Sugarcane streak mosaic virus infecting sugarcane in Indonesia. J. Gen. Plant Path., 77: 72-74. Doi 10.1007/s10327-010-0285-7. [Google Scholar]
  • Datnoff, L.E., Elmer, W.H., and Huber, D.M. (2007). Mineral nutrition and plant disease-American Phytopathological Society, J. Plant Prot. Res., 48(1): 106. Doi: 10.2478/v10045-008-0016-7. [Google Scholar]
  • Dordas, C. (2008). Role of nutrients in controlling plant diseases in sustainable agriculture. A review. Agron. Sustain. Dev., 28: 33-46. Doi: 10.1051/agro:2007051. [Google Scholar]
  • Easterday, C.A., Kendig, A.E., Lacroix, C., Seabloom, E.W., Borer, E.T. (2022). Long-term nitrogen enrichment mediates the effects of nitrogen supply and co-inoculation on a viral pathogen. Ecol. Evol., 12(1): e8450. https://doi.org/10.1002/ece3.8450. [Google Scholar] [Crossref] 
  • Elmer, W.H., Datnoff, L.E. (2014). Mineral Nutrition and Suppression of Plant Disease. In: Neal VA. Encyclopedia of Agriculture and Food Systems, (s. 231-244). San Diego. Elsevier. [Google Scholar]
  • Fageria, N.K. (2009). The Use of Nutrients in Crop Plants. New York: CRC Press [Google Scholar]
  • Hosseini, S.A., Zamani, G.R., Yaghub, Z.M. and Khayyat, M. (2018). Effects of Cucumber Mosaic Virus infection and drought tolerance of tomato plants under greenhouse conditions: Preliminary results. J. Berry Res., 8(2): 129-136. Doi: 10.3233/JBR-170285. [Google Scholar]
  • Iriti, M. and Faoro, F. (2008). Abscisic acid is involved in chitosan-induced resistance to tobacco necrosis virus (TNV). Plant Physiol. Biochem., 46: 1106-1111. https://doi.org/10.1016/j.plaphy.2008.08.002. [Google Scholar] [Crossref] 
  • Kendig, A. E., Borer, E. T., Boak, E. N., Picard, T. C., Seabloom, E. W. (2020). Host nutrition mediates interactions between plant viruses, altering transmission and predicted disease spread. Ecology, 101(11): e03155. https://doi.org/10.1002/ecy.3155. [Google Scholar] [Crossref] 
  • Kormelink, R. (2005) Tomato spotted wilt virus. In: Description of plant viruses (DPV) no. 412, https://www.dpvweb.net/dpv/showdpv/?dpvno=412/ (accessed on 5 September 2024). [Google Scholar]
  • Krisha-Kumar, N.K., Ullman, D.E., and Cho, J.J. (1993). Evaluation of lycopersicon germ plasm for Tomato sotted wilt tospovirus resistance by mechanical and trips transmission. Plant Dis., 77(9): 938-941. [Google Scholar]
  • Labuschagne, P.M. (1995). Casing media for Agaricus bisporus cultivation in South Africa (Doctoral dissertation, University of Pretoria). [Google Scholar]
  • Li, W., Cui, X., Meng, Z., Huang, X., Xie, Q., Wu, H., et al. (2012). Transcriptional regulation of Arabidopsis MIR168a and argonaute1 homeostasis in abscisic acid and abiotic stress responses. Plant Physiol., 158:1279-1292. https://doi.org/10.1104/pp.111.188789. [Google Scholar] [Crossref] 
  • Mandal, B., Pappu, H. R., Culbreath, A. K. (2001). Factors affecting mechanical transmission of Tomato spotted wilt virus to peanut (Arachis hypogaea). Plant Dis., 85(12): 1259-1263. https://doi.org/10.1094/PDIS.2001.85.12.1259. [Google Scholar] [Crossref] 
  • Mandal, B., Csinos, A.S., Martinez-Ochoa, N. and Pappu, H.R. (2008). A rapid and efficient inoculation method for Tomato spotted wilt tospovirus. J. Virol. Methods, 149(1): 195-198. Doi: 10.1016/j.jviromet.2007.12.007. [Google Scholar]
  • Marschner, H. (1995). Mineral Nutrition of Higher Plants. London: Academic Press. 889 pp. [Google Scholar]
  • Marschner, H., and Marschner, P. (2012). Marschner’s Mineral Nutrition of Higher Plants, San Diego: Elsevier Academic Press. 1–651. [Google Scholar]
  • Mur, L.A., Simpson, C., Kumari, A., Gupta, A.K. and Gupta, K.J. (2017). Moving nitrogen to the centre of plant defence against pathogens. Ann. Bot., 119(5): 703-709. Doi: 10.1093/aob/mcw179. [Google Scholar]
  • Mutebi, C.M. and Moranga, V. (2022). Effect of Different Soil Fertilities on Cowpea Mosaic Virus Disease Incidence. Int. J. Hortic. Sci. Technol., 9(3): 355-362. https://DOI: 10.22059/IJHST.2021.331384.507. [Google Scholar]
  • Pataky, N.R. (1991). Tomato spotted wilt virus. Report on Plant Disease, RPD No. 665, Department of Crop Sciences, University of Illinois, Urbana Champaign. [Google Scholar]
  • Putra, L.K. and Damayanti, T.A. (2016). Effect of nitrogen and potassium fertilizers, and soil water content on incidence and severity of Sugarcane streak mosaic virus (SCSMV) infecting sugarcane. In Proceedings of the International Society of Sugar Cane Technologists, 29: 1620-1626. [Google Scholar]
  • Reynolds, W. D., Bowman, B. T., Drury, C. F., Tan, C. S., & Lu, X. (2002). Indicators of good soil physical quality: density and storage parameters. Geoderma, 110(1-2): 131-146. https://doi.org/10.1016/S0016-7061(02)00228-8. [Google Scholar] [Crossref] 
  • Roselló, S., Díez, M.J. and Nuez, F. (1996). Viral diseases causing the greatest economic losses to the tomato crop. I. The Tomato spotted wilt virus-a review. Sci. Hortic., 67(3-4): 117-150. https://doi.org/10.1016/S0304-4238(96)00946-6. [Google Scholar] [Crossref] 
  • Scholthof, K.B.G., Adkins, S., Czosnek, H., Palukaitis, P., Jacquot, E., Hohn, T., et al. (2011). Top 10 plant viruses in molecular plant pathology. Mol. Plant Path., 12(9): 938-954. Doi: 10.1111/j.1364-3703.2011.00752.x. [Google Scholar]
  • Sun, Y., Wang, M., Mur, L. A. J., Shen, Q. and Guo, S. (2020). Unravelling the roles of nitrogen nutrition in plant disease defences. Int. J. Mol. Sci., 21(2): 572. https://doi.org/10.3390/ijms21020572. [Google Scholar] [Crossref] 
  • Suzuki, N., Rivero, R.M., Shulaev, V., Blumwald, E., Mittler, R. (2014). Abiotic and biotic stress combinations. New Phytol., 203: 32-43. https://doi.org/10.1111/nph.12797. [Google Scholar] [Crossref] 
  • Takahashi, F., Kuromori, T., Urano, K., Yamaguchi-Shinozaki, K., Shinozaki, K. (2020). Drought Stress Responses and Resistance in Plants: From Cellular Responses to Long-Distance Intercellular Communication. Front. Plant Sci., 11. https://doi.org/10.3389/FPLS.2020.556972. [Google Scholar] [Crossref] 
  • Uyemoto, J.K., Luhn, C.F., Asai, W.K., Beede, R., Beutel, J.A., Fenton, R. (1989). Incidence of ilarviruses in young peach trees in California. Plant Dis., 73(3): 217-20. Doi:10.1094/PD-73-0217, 0191-2917. [Google Scholar]
  • Walters, D.R., and Bingham, I.J. (2007). Influence of nutrition on disease development caused by fungal pathogens implications for plant disease control. Ann. App. Biol., 151(3): 307-324. https://doi.org/10.1111/j.1744-7348.2007.00176.x. [Google Scholar] [Crossref]