International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article    |    Open Access
International Journal of Innovative Approaches in Agricultural Research 2024, Vol. 8(3) 218-229

Chemical Composition, Antioxidant and Antimicrobial Activities of Essential Oil Extracted from waste of Juniperus communis L.  Medicinal and Aromatic Plants Industry in Albania

Elton Basha, Erjon Mamoçi, Entela Hodaj-Çeliku, Aniket Sharma & Bisha Bledar

pp. 218 - 229   |  DOI: https://doi.org/10.29329/ijiaar.2024.1075.4

Published online: September 30, 2024  |   Number of Views: 7  |  Number of Download: 47

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Abstract

The Medicinal and Aromatic Plants (MAPs) industry is relevant for Albania covering approximately 20% of agricultural exports. Mostly high quality dried parts of MAPs are traded, while products not fulfilling quality criteria and non-tradable plant parts are often regarded as waste products, even though they contain valuable bioactive substances. This study evaluates the composition and biological activity of the essential oil (EO) fraction of wastes generated from the MAPs industy of Juniperus communis L. in Albania.

Juniperus communis L. was collected from Korçë area, dried, screened for trade quality berries in an industrial plant for MAPs, and the waste parts underwent hidrodistilation in industrial distilators. Chemical composition was performed using Gas Chromatography coupled with Mass Spectrometry and identified 50 compounds, where main components were α-Pinene (24.47%), Sabinene (12.4%), Germacrene D (3.2%) and β-Myrcene (1.6%). The antioxidant capacity of J. communis L. EO was determined by 2,2-diphenyl-1-picrylhydrazil (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and expressed as Inhibitory Concentration of 50% of the free radical (IC50) where values were IC50= 155.4 µg/mL and IC50= 163.2µg/mL for DPPH and ABTS respectively. Antimicrobial activity of J. communis L. EO was determined against five bacteria, Escherichia coli ATCC 10535; Salmonella enteritidis, ATCC 49223, Pseudomonas aeruginosa, ATCC 9027; Micrococcus luteus, ATCC 10240; Stenotrophomonas maltophilia, ATCC 1363;and one yeast, Candida albicans, ATCC 10231 by microdilution method used to determine the minimum inhibitory concentration (MIC). The EO showed no antimicrobial activity against the first 3 bacterial strains, while it inhibited growth of Micrococcus luteus and Candida albicans at concentrations of 2.5 mg/mL and of Stenotrophomonas maltophilia at the maximum tested concentration of 5 mg/mL.

Keywords: Juniperus communis L. by-product, antioxidant, antimicrobial, MIC

How to Cite this Article

APA 6th edition
Basha, E., Mamoci, E., Hodaj-Celiku, E., Sharma, A. & Bledar, B. (2024). Chemical Composition, Antioxidant and Antimicrobial Activities of Essential Oil Extracted from waste of Juniperus communis L.  Medicinal and Aromatic Plants Industry in Albania . International Journal of Innovative Approaches in Agricultural Research, 8(3), 218-229. doi: 10.29329/ijiaar.2024.1075.4

Harvard
Basha, E., Mamoci, E., Hodaj-Celiku, E., Sharma, A. and Bledar, B. (2024). Chemical Composition, Antioxidant and Antimicrobial Activities of Essential Oil Extracted from waste of Juniperus communis L.  Medicinal and Aromatic Plants Industry in Albania . International Journal of Innovative Approaches in Agricultural Research, 8(3), pp. 218-229.

Chicago 16th edition
Basha, Elton, Erjon Mamoci, Entela Hodaj-Celiku, Aniket Sharma and Bisha Bledar (2024). "Chemical Composition, Antioxidant and Antimicrobial Activities of Essential Oil Extracted from waste of Juniperus communis L.  Medicinal and Aromatic Plants Industry in Albania ". International Journal of Innovative Approaches in Agricultural Research 8 (3):218-229. doi:10.29329/ijiaar.2024.1075.4.
References
  1. AAFS (2019). Medicinal and Aromatic Plants Sector Study. Albania Agribusiness Support Facility (AASF). Institute of Economics Studies and Knowledge Transfer Tirana, 2019. Available online at: https://aasf.com.al/wp-content/uploads/2019/08/Map-EN.pdf [Google Scholar]
  2. Sela, F.; Karapandzova, M.; Stefkov, G.; Cvetkovikj, I.; Trajkovska-Dokik, E.; Kaftandzieva, A.; Kulevanova, S. Chemical composition and antimicrobial activity of leaves essential oil of Juniperus communis (Cupressaceae) grown in Republic of Macedonia. Maced. Pharm. Bull. 2013, 59, 23–32. [Google Scholar]
  3. Adams R.P.,(2007) Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. In: Forth Edition. Allured Publishing Corporation, Carol Stream, IL. [Google Scholar]
  4. Agastra, A.; Gixhari, B.; Kadiasi, N.; Ibraliu, A. Influence of environmental factors in the composition of essential oils content ofJuniperus communis L. berries, in Southeast part of Albania. Int. J. Ecosyst. Ecol. 2021, 11, 943–948. [Google Scholar]
  5. Ainane, A., Abdoul-Latif, F. M., Ali, A. M., Mohamed, J., Shybat, Z. L., & Ainane, T. (2022). Chemical composition ofJuniperus communis L. essential oil and evaluation of its antifungal activity in vitro against Ascochyta rabiei. Journal ofAnalytical Sciences and Applied Biotechnology, 4(2), 108-115. [Google Scholar]
  6. Angioni A, Barra A, Russo MT, Coroneo V, DessiS, Cabras P. Chemical composition of the essentialoils of Juniperus from ripe and unripe berriesand leaves and their antimicrobial activity. J AgricFood Chem 2003; 51(10):3073-3078. doi: [Google Scholar]
  7. Brand-Williams W, Cuvelier ME, Berset C: Use of free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft und-Technol 1995, 28:25–30. [Google Scholar]
  8. Buci, A.; Hodaj Celiku, E.; Manaj, H.; Abazi, S.; Drushku, S.; Lazari, D. Essential oilcomposition from Juniperus communis originated from Albania. Int. J. Environ. Appl. Sci.2018, 13, 15-19. [Google Scholar]
  9. Code of Federal Regulation. 21 CFR Part 182–Substances Generally Recognized as Safe (4–1–23 Edition). 2023. Availableonline: https://www.govinfo.gov/content/pkg/CFR-2023-title21-vol3/pdf/CFR-2023-title21-vol3-part182.pdf [Google Scholar]
  10. Damjanowić B, Skala D, Baras J, Petrović-DjakovD. Isolation of essential oil and supercritical carbondioxide extract of Juniperus communis L fruitsfrom Montenegro. Flavour Fragr J 2006; 21:875-880. doi: [Google Scholar]
  11. Emami, S.A.; Javadi, B.; Hassanzadeh, M.K. Antioxidant activity of the essential oils of differentparts of Juniperus communis. subsp. hemisphaerica and Juniperus oblonga. Pharm. Biol. 2007,45, 769–776. [Google Scholar]
  12. Enescu, C.M.; Houston Durrant, T.; Caudullo, G.; de Rigo, D. Juniperus communis in Europe: Distribution, habitat, usage and threats. In European Atlas of Forest Tree Species; San-Miguel-Ayanz, J., de Rigo, D., Caudullo, G., Houston Durrant, T., Mauri, A., Eds.; Publications Office of the EU: Luxembourg, 2016; p. e01d2de+. [Google Scholar]
  13. Esteban LS, Mediavilla I, Xavier V, Amaral JS, Pires TCSP, Calhelha RC, López C, Barros L. Yield, Chemical Composition and Bioactivity of Essential Oils from Common Juniper (Juniperus communis L.) from Different Spanish Origins. Molecules. 2023 May 30;28(11):4448. [Google Scholar]
  14. Höferl M, Stoilova I, Schmidt E, Wanner J, Jirovetz L, Trifonova D et al. Chemical compositionand antioxidant properties of Juniper berry(Juniperus communis L.) essential oil on the antioxidantprotection of Saccharomyces cerevisiaemodel organism. Antioxidants 2014; 3:81-98. [Google Scholar]
  15. Joint FAO/WHO Codex Alimentarius Commission. Codex Alimentarius:CX/SCH 21/5/3 On Spices and Culinary Herbs. Rome: World HealthOrganization: Food and Agriculture Organization of the United Nations (2021). [Google Scholar]
  16. Koukos PK, Papadopoulou KI. Essential oil of Juniperuscommunis L. grown in Northern Greece:variation of fruit yield and composition. J EssentOil Res 1997; 9:35-39. doi: [Google Scholar]
  17. M100 Ed34 | Performance Standards for Antimicrobial Susceptibility Testing, 34th Edition. Clinical & Laboratory Standards Institute. https://clsi.org/standards/products/microbiology/documents/m100/ [Google Scholar]
  18. Mancuso, G.;Midiri, A.; Gerace, E.; Biondo, C. Bacterial Antibiotic Resistance: TheMost Critical Pathogens. Pathogens 2021, 10, 1310. [Google Scholar]
  19. MassadaY., (1976) Analysis of essential oils by Gas Chromatography and Mass Spectrometry: John Wiley & Sons, New York. [Google Scholar]
  20. Medicinal and Aromatic Plants Sector Study. Selected sectorial analysis as a solid ground for the preparation of IPARD III programme and of Strategy for Agriculture, Rural Development andFishery 2021-2027 for Albania. May 2021. [Google Scholar]
  21. Mërtiri, I.; Păcularu-Burada, B.; Stănciuc, N. Phytochemical Characterization and Antibacterial Activity of Albanian Juniperus communis and Juniperus oxycedrus Berries and Needle Leaves Extracts. Antioxidants 2024, 13, 345. [Google Scholar]
  22. Navarrete A, Herrero M, Martin A, Cocero MJ. Ibanez, E. Valorizationof solid wastes from essential oil industry. J Food Eng. (2011) 104:196–201. [Google Scholar]
  23. Puškárová A, Bučková M, Kraková L, Pangallo D, Kozics K. The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells. Sci Rep 2017;7:8211. [Google Scholar]
  24. Rao J, Chen B, McClements DJ. Improving the Efficacy of Essential Oils as Antimicrobials in Foods: Mechanisms of Action. Annu Rev Food Sci Technol. 2019 Mar 25;10:365-387. [Google Scholar]
  25. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999 May;26(9-10):1231-7. [Google Scholar]
  26. Roberto, D.; Micucci, P.; Sebastian, T.; Graciela, F.; Anesini, C. Antioxidant activity of limoneneon normal murine lymphocytes: Relation to H2O2 modulation and cell proliferation. Basic Clin.Pharmacol. Toxicol. 2010, 106, 38–44 [Google Scholar]
  27. Routray W, Orsat V. “Plant By-Products and Food Industry Waste: A Sourceof Nutraceuticals and Biopolymers,” In: Grumezescu M, Holban AM, editors.Handbook of Food Bioengineering, Food Bioconversion. (2017).  279–315. [Google Scholar]
  28. Sahaa A, Basaka BB. Scope of value addition and utilization of residualbiomass from medicinal and aromatic plants. Ind Crops Prod. (2020) 145:111979. [Google Scholar]
  29. Salamon I, Ibraliu A , Fejer J, (2014) Essential oil of common juniper (Juniperus communis L.) In Albania. Conference paper : Proceedings of the Eighth Conference on Medicinal and Aromatic Plants of Southeast European Countries (8th CMAPSEEC) 19-22 May 2014, Durrës, Albania, pp.239-244. [Google Scholar]
  30. Sela, F.; Karapandzova, M.; Stefkov, G.; Cvetkovikj, I.; Trajkovska-Dokik, E.; Kaftandzieva, A.; Kulevanova, S. Chemical composition and antimicrobial activity of leaves essential oil of Juniperus communis (Cupressaceae) grown in Republic of Macedonia. Maced. Pharm. Bull. 2013, 59, 23–32. [Google Scholar]
  31. Shamir F, Ahmadi L, Mirza M, Korori SAA. Secretoryelements of needles and berries of Juniperuscommunis L. ssp. communis and its volatileconstituents. Flavour Frag J 2003; 18:425-428. [Google Scholar]
  32. Van den Dool H and Kratz PD, (1963) A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography 11, 463. [Google Scholar]
  33. Wang Q, Rehman M, Peng D, Liu L. Antioxidant capacity and a-glucosidaseinhibitory activity of leaf extracts from ten ramie cultivars. Ind Crop Prod. (2018) 122:430–7. [Google Scholar]
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