Investigation of the Effectiveness of Inoculation Methods of <em>Macrophomina Phaseolina</em>, the Causative Agent of Coal Rot Disease, in Soybean

Sema Şahbaz, Fatih Mehmet Tok & Yaşar Akışcan

doi:https://doi.org/10.29329/ijiaar.2024.1049.1

Original article | Open Access
International Journal of Innovative Approaches in Agricultural Research 2024, Vol. 8(2) 62-72

Investigation of the Effectiveness of Inoculation Methods of Macrophomina Phaseolina, the Causative Agent of Coal Rot Disease, in Soybean

Sema Şahbaz, Fatih Mehmet Tok & Yaşar Akışcan

pp. 62 - 72 | DOI: https://doi.org/10.29329/ijiaar.2024.1049.1

Published online: June 30, 2024 | Number of Views: 0 | Number of Download: 2

Download PDF

Abstract

Although soybean is affected by many fungal diseases from seed to harvest, charcoal rot caused by Macrophomina phaseolina is one of the most important diseases of soybean. It is important to grow resistant varieties because microsclerots can remain alive in the soil for many years, and there is currently no effective chemical control. Currently, many inoculation methods are used to determine the resistance of soybean plants to M. phaseolina; however, their effectiveness under field conditions has not been fully determined. In this study, soybean plants were inoculated with M. phaseolina during the flowering period using agar-disc, toothpick, and microsclerot injection methods, and the efficiency of these methods was investigated. In the disease observations made close to harvest, the disease index value was 3.3 in the agar disk method, 1.9 in the toothpick method and 1.3 in the microsclerot method. The results of the study showed that the symptoms in all methods were similar to those in field conditions, the agent could be transmitted by seeds and had an effect on seed quality, and the agar-disc method was the most successful method among the methods used to inoculate soybean plants with M. phaseolina under field conditions.

Keywords: Soybean, Disease, Macrophomina, Charcoal rot, Resistance

How to Cite this Article

APA 6th edition
Sahbaz, S., Tok, F.M. & Akiscan, Y. (2024). Investigation of the Effectiveness of Inoculation Methods of Macrophomina Phaseolina, the Causative Agent of Coal Rot Disease, in Soybean . International Journal of Innovative Approaches in Agricultural Research, 8(2), 62-72. doi: 10.29329/ijiaar.2024.1049.1

Harvard
Sahbaz, S., Tok, F. and Akiscan, Y. (2024). Investigation of the Effectiveness of Inoculation Methods of Macrophomina Phaseolina, the Causative Agent of Coal Rot Disease, in Soybean . International Journal of Innovative Approaches in Agricultural Research, 8(2), pp. 62-72.

Chicago 16th edition
Sahbaz, Sema, Fatih Mehmet Tok and Yasar Akiscan (2024). "Investigation of the Effectiveness of Inoculation Methods of Macrophomina Phaseolina, the Causative Agent of Coal Rot Disease, in Soybean ". International Journal of Innovative Approaches in Agricultural Research 8 (2):62-72. doi:10.29329/ijiaar.2024.1049.1.

References

Akışcan, Y., & Tok, F.M. (2019). Farklı Kökenli Bazı Pamuk Genotiplerinde Verticillium Solgunluğuna Dayanıklılığın Araştırılması. KSÜ Tarım ve Doğa Derg 22(Ek Sayı 2): 354-360. [Google Scholar]
Anonim, 2002. Fao istatistik Bölümü İnternet sitesi. http://www.fao.org. [Google Scholar]
Arca, G., & Yıldız, M. (1990). Investigaıions on the incıdence of tobacco charçoal rot disease (Macrophomina phaseolina (Tassi) Goid.) in the Aegean Region, iıs paıîıogenicity and susceptibility of Turkish lobacco cullivars. J. Turk. Phytopath., 19: 13-19. [Google Scholar]
Arıoğlu, H. (1999). Yağ Bitkileri Yetiştirme ve Islahı, Çukurova Üniversitesi Ziraat Fakültesi Genel Yayın No: 220, Ders Kitapları Yayın No: A-70, Adana [Google Scholar]
Arıoğlu, H. (2007). Yağ Bitkileri Yetiştirme ve Islahı Ders Kitapları Yayın No:A70, Çukurova Üniversitesi Ziraat fakültesi Ofset Atölyesi, 204 s., Adana. [Google Scholar]
Avilés, M., Castillo, S., Bascon, J., Zea-Bonilla, T., Martín-Sánchez, PM., & Pérez-Jiménez, RM. (2008). First report of Macrophomina phaseolina causing crown and root rot of strawberry in Spain. Plant Pathol. 57(2):382. [Google Scholar]
Booth C. The genus Fusarium. Common wealth Mycological Institute. Kew. Surrey. England 1971, pp 237. [Google Scholar]
Cohen, R., Elkabetz, M., & Edelstein, M. (2016). Variation in the responses of melon and watermelon to Macrophomina phaseolina Department of Plant Sciences, Agricultural Research Organization, Newe Ya'ar Research Center, Ramat Yishay, 30095, Israel [Google Scholar]
Deniz, N. (1988). Ankara Yöresinde Sulu Koşullarda Yetiştirilebilecek Soya Çeşitleri. Tarım Orman ve Köy İşl. Bak., Köy Hiz. Gen. Md., Toprak ve Gübre Araş. Enst. Md. Genel Yayın No: 148, Rapor Seri No: R-72. Ankara. [Google Scholar]
Diourte, M., S, J, Star., M. J. Jeger., J. P. Stack & D. T. Rosenow (1995). Charcoal Rot (Macrophomina phaseolina) Resistance and the Effect of Water Stress on Disease Development in Sorghum. Plant Pathology, 44: 196- 202. [Google Scholar]
Esentepe, M., Onan, E., Sezgin, E., & Karcılıoğlu, A., (1985). Investigaıions on sesame seed bome fungi and their rates of presence. J. Turk. Phytopath., 14: 95-96 (Abst.). [Google Scholar]
GerJach, W., & Nirenberg, HI. (1982) The genus Fusarium — a pictorial atlas. Mitt Biol Bundesanst Land- Forstwirtsch, Berlin-Dahlem 209: 406 pp [Google Scholar]
Hussain, S., Ghaffar A., & Aslam, M.,(1990). Biological control of Macrophomina phaseolina ehîffCCatfOq£ sunflowerandMung bean. J. Phytopalhology, 130: 157-160. [Google Scholar]
İlisulu, K., (1983). Soyanın Türkiye Ekonomisindeki Yeri ve Önemi. Soya Semineri ve Paneli. Adana. [Google Scholar]
Karcılıoğlu, A., Esentepe M., Onan, E., & Sezgin, E., (1990). Ege Bölgesi ikinci iirün ekim alanlarında göriilen hastalık, zararlı, yabancı otlar ve bunların doğal düşmanları üzerinde araştırmalar. Bornova Zirai Mücadele Araştırma Enstitüsü, 8/A 600.001.1 No'lu proje nihai raporu (Yayınlanmamış). [Google Scholar]
Maden, S., (1987). Seed-bome fungal diseases af chick-pea in Turkey. J. Turk. Phytopath., 16: 1-8. [Google Scholar]
Maden, S., & İren, S., (1984). Fasulyelerde tohumla geçen bazı önemli fungal hastalık etmenlerinin tanımlanması, taşınma şekilleri ve mücadele yöntemleri üzerinde araştırmalar. Ankara Üniversitesi Fen Bilimleri Enstitüsü, Yayın No BK.2: 1-15. [Google Scholar]
Malone, GP., & Muskette, AE. (1964). Seed borne fungi: Description of 77 fungal species. Proc. Int.Seed Test Assoc 29(2):180-183. [Google Scholar]
Onan, E., Çimen, M., & Karcılıoğlu, A. (1992). Fungal diseases of sunflovver in Aegean Region of Türkiye. J. Turk. Phytopath., 21: 101-107. [Google Scholar]
Öz, S., & Yalçın 0. (1991). Ege Bölgesinde yemlik baklagillerde görülen fungal hastalıklar üzerinde ön çalınmalar. VI, Türkiye Fitopatoloji Kongresi (7-11 Ekim 1991, İzmir), Türkiye Rhizoctonia-like fungi from turfgrasses in North Carolina. Phytopathology, 74: 170-175 [Google Scholar]
Pickel, B., Dai, N., Maymon, M., Elazar, M., Tanami, Z., Frenkel, O., Toamy, MA., Mor, N., & Freeman, S. (2020). Development of a reliable screening technique for determining tolerance to Macrophomina phaseolina in strawberry. Eur J Plant Pathol. 157(7):707–718. [Google Scholar]
Pratt, RG., Mclaughlin, MR., Pederson, GA., & Rowe, DE. (1998). Pathogenicity of Macrophomina phaseolina to mature plant tissues of alfalfa and white clover. Plant Dis. 82(9):1033–1038. [Google Scholar]
Temizel, M., & Erlunç, F. (1992). Investigations on the detection of bean diseases of Van Province. J. Türk. Phytopath., 21: 25-31. [Google Scholar]
Tezcan, H, & Yıldız, M., (1993). Invesligations on the collapse of melon planls caused by soil-bome fungi in Turkey. 6 ıh International Congress of Plant Paıhology (July 28- August 6, 1993), Montreal (Quebcc) Canada, 143 (Abst). [Google Scholar]
Tezcan, H., Demir, S., & Çiftçi, V. (1994). Broad bean: a new host record for Macrophomina phaseoiina charcoal rot in Türkiye. 9 th Congress of the Mediterranean Phyiopalhotogieal Union (Sepıember 18-24, 1994, Kuşadası-Aydın), Turkish Phytopathologieal Society Pııblication, No 7: 535-536. [Google Scholar]
Twizeyimana, M., Hill, CB., Pawlowski, M., Paul, C., & Hartman, GL. (2012). A cut-Stem inoculation technique to evaluate soybean for resistance to Macrophomina phaseolina. Plant Dis. 96(8):1210–1215. [Google Scholar]
Ulukış, I. (1978). Macrophomina phaseoli (Maubl.) Ashby'nm soğandan elde edilen izolatınm suda erir kırmızı pigment oluşturmasına elverişli yeni bir ortam. II. Türkiye Fitopatoloji Kongresi (9-13 Ekim 1978, Ankara), Tebliğ Özetleri, 10. [Google Scholar]
Viteri, DM., & Linares, AM. (2017). Reaction of Phaseolus spp. genotypes to ashy stem blight caused by Macrophomina phaseolina. Euphytica. 213(8):1–8. [Google Scholar]
Yorgancı, Ü., & Turhan, G. (1988). Untersuchungen über den weicfıfaeule erzeugenden erregerkompleı an zuckerrüben. J, Turk. Phytopath., 17: 57-66. [Google Scholar]
Yıldız, M. (1989). Domateslerde Fungal Hastalıklar. Domateslerde Hastalıklar, Zararlılar ve Yabancıotlar, İstanbul, Hasad, 35-50. [Google Scholar]
Yücel, S., & Günci , M.(1991). Akdeniz Bölgesi yemeklik baklagillerinde görülen fungal hastalıklar. Bitki Koruma Bülteni, 31: 19-30. [Google Scholar]
Zazzerini A, Tosi L. (1989). Chlorate sensitivity of Sclerotium bataticola isolates from different hosts. J Phytopathol. 126(3):219–224. [Google Scholar]

Volume 8 Issue 2

June 2024

All Articles