Development and Validation of Analytical Methods for Quantification of L-Dopa; Application to Plant Materials

Erten Akbel & İbrahim Bulduk

doi:https://doi.org/10.29329/ijiaar.2023.568.5

Original article | Open Access
International Journal of Innovative Approaches in Agricultural Research 2023, Vol. 7(2) 208-219

Development and Validation of Analytical Methods for Quantification of L-Dopa; Application to Plant Materials

Erten Akbel & İbrahim Bulduk

pp. 208 - 219 | DOI: https://doi.org/10.29329/ijiaar.2023.568.5

Published online: June 29, 2023 | Number of Views: 45 | Number of Download: 170

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Abstract

An essential neurotransmitter used to treat nervous system dysfunction like Parkinson's disease is L-dopa. The amount of L-dopa in plant materials has been measured in this study using chromatographic and spectrophotometric methods that are user-friendly, straightforward, quick, and affordable. Utilizing conventional instrumental parameters, high-performance liquid chromatography and spectrophotometry methods were established. In accordance with ICH guidelines, these analytical methods were validated for linearity, accuracy, precision, and robustness. The chromatographic procedure made use of an Agilent Extend C18 (250×4.6 mm, 5 µm) column. 0.1% trifloroacetic acid solution and acetonitrile (92/8, v/v) were used as mobile phase. It was run in isocrotic mode and the flow rate was 1 mL min-1. The retention time of L-dopa was determined as 3.85 minutes. L-dopa was identified using the spectrophotometric method, which involved measuring the solutions' absorbance at a wavelength of 280 nm. In spectrophotometric analysis, ultra-pure water as a solvent gave sufficient molar absorptivity at a λmax of 280 nm. The results showed that spectrophotometric and chromatographic methods were linear, accurate, precise, robust, and the percent recovery was within standard limits. No statistically significant distinction existed between the methods within the 95% confidence interval (p<0.05). The developed methods can be used to quantify L-dopa in plant materials for routine analysis and have been found to be very efficient.

Keywords: L-Dopa, Quantification, Chromatography, Spectrophotometry, Validation

How to Cite this Article

APA 6th edition
Akbel, E. & Bulduk, I. (2023). Development and Validation of Analytical Methods for Quantification of L-Dopa; Application to Plant Materials . International Journal of Innovative Approaches in Agricultural Research, 7(2), 208-219. doi: 10.29329/ijiaar.2023.568.5

Harvard
Akbel, E. and Bulduk, I. (2023). Development and Validation of Analytical Methods for Quantification of L-Dopa; Application to Plant Materials . International Journal of Innovative Approaches in Agricultural Research, 7(2), pp. 208-219.

Chicago 16th edition
Akbel, Erten and Ibrahim Bulduk (2023). "Development and Validation of Analytical Methods for Quantification of L-Dopa; Application to Plant Materials ". International Journal of Innovative Approaches in Agricultural Research 7 (2):208-219. doi:10.29329/ijiaar.2023.568.5.

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