International Journal of Innovative Approaches in Agricultural Research
Abbreviation: IJIAAR | ISSN (Online): 2602-4772 | DOI: 10.29329/ijiaar

Original article    |    Open Access
International Journal of Innovative Approaches in Agricultural Research 2020, Vol. 4(1) 56-68

Antifungal Activity of Mentha Rotundifolia Essential Oil Against Fusarium Oxysporum

Amina Leblalta, Harzallah Daoud, Cherrad Semcheddine & İbrahim Demirtaş

pp. 56 - 68   |  DOI: https://doi.org/10.29329/ijiaar.2020.238.7

Published online: March 29, 2020  |   Number of Views: 210  |  Number of Download: 630

Download PDF

Abstract

The antifungal activity of Mentha rotundifolia essential oil, harvested in Setif (Algeria) was evaluated in vitro against a phytopathogenic fungi Fusarium oxysporum, causing damage on tomato. The molecular identification of the strain was based on a comparison (BLAST) of the sequences obtained against a database and was often supplemented by microscopic observations. After "SANGER" sequencing of the PCR products, the sequences were received in FASTA format. Analysis of M. rotundifolia essential oil by Gas Chromatography/Mass Spectrometry method (GC-MS) identified 14 compounds.  The 3-Cyclopenten-1-one, 2-hydroxy-3- (3-methyl-2-butenyl) - was the major constituent of this oil with a rate of about 89.09%. For this activity, we adopted the technique of direct contact on agar. F. oxysporum continued to grow on oil-free media at 1% and 0.1% (fungistatic effect); also on media with an oil concentration of 0.01%. While the explants taken from petri dish with essential oil concentration of 2; 4 and 10% did not grow (fungicidal effect). The very interesting antifungal effect of M. rotundifolia essential oil indicates the potential of this plant species as a source of natural fungicidal material. The present study revealed that this mint exhibited antifungal effect against F. oxysporum which provided a scientific basis for the use of this species as a good source of antifungal compounds. This preliminary work could provide a basis for the determination of sufficient and effective concentrations for in planta studies for the biological control of natural active substances of M. rotundifolia against fungal diseases.

Keywords: Antifungal activity, Mentha rotundifolia, essential oil, Fusarium oxysporum, molecular identification, GC/MS.

How to Cite this Article

APA 6th edition
Leblalta, A., Daoud, H., Semcheddine, C. & Demirtas, I. (2020). Antifungal Activity of Mentha Rotundifolia Essential Oil Against Fusarium Oxysporum . International Journal of Innovative Approaches in Agricultural Research, 4(1), 56-68. doi: 10.29329/ijiaar.2020.238.7

Harvard
Leblalta, A., Daoud, H., Semcheddine, C. and Demirtas, I. (2020). Antifungal Activity of Mentha Rotundifolia Essential Oil Against Fusarium Oxysporum . International Journal of Innovative Approaches in Agricultural Research, 4(1), pp. 56-68.

Chicago 16th edition
Leblalta, Amina, Harzallah Daoud, Cherrad Semcheddine and Ibrahim Demirtas (2020). "Antifungal Activity of Mentha Rotundifolia Essential Oil Against Fusarium Oxysporum ". International Journal of Innovative Approaches in Agricultural Research 4 (1):56-68. doi:10.29329/ijiaar.2020.238.7.
References
  1. Alexopoulos, CJ, Mims CW., (1979). Introductory Mycology (3rd ed). John Wiley & Sons: New York. [Google Scholar]
  2. Arab R., Bounechada M., Ramdani M., (2016). Chemical composition and insecticidal activity of Mentha rotundifolia L. on Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) from Algeria. Adv. Environ. Biol., 10 (10), 13-19. [Google Scholar]
  3. Aziz, E. E. and M. H. Abbass (2010). Chemical composition and efficiency of five essential oils against the pulse beetle Callosobruchus maculatus (F.) on Vigna radiata seeds. Am. Eurasian J. Agric. Environ. Sci., 8 (4), 411- 419. [Google Scholar]
  4. Ben-Bnina, E., S. Hammami, M. Daamii-remadi, H. Ben-Jannet and Z. Mighri (2010). Chemical composition and antimicrobial effects of Tunisian Ruta chalepensis L. essential oils. J. Soc. Chim. Tunisie, 12, 1–9. [Google Scholar]
  5. Bitar, D., O. Lortholary, F. Dromer, B. Coignard and D. Che (2013). Mycoses invasives et France Métropolitaine, PMSI, incidence, létalité et tendances 2001–2010. Bulletin épidémiologique hebdomadaire, 109 -114. [Google Scholar]
  6. Botton, B., A. Breton, M. Févre, S. Gauthier, Ph. Guy, J.-P. Larpent, P.  Reymond, J. J.  Sanglier, Y. Vayssier and R. Veau (1990). Moisissures Utiles Et Nuisibles : Importance Industrielle. 2éme Edition Masson, Paris. 442 p. [Google Scholar]
  7. Brada, M., M. Bezzina, M. Marlier and G. C. Lognay (2006). Chemical Composition of the Leaf oil of Mentha rotundifolia (L.) from Algeria. J. Essen. Oil Res., 18, 663-665. [Google Scholar]
  8. Brada, M., M. Bezzina, M. Marlier, A. Carlier and G. Lognay (2007). Variabilité de la composition chimique des huiles essentielles de Mentha rotundifolia du Nord de l’Algérie. J. Biotechnol. Agron. Soc. Environ., 11, 3–7. [Google Scholar]
  9. Brahmi, F., A. Adjaoud, B. Marongiu, S. Porcedda, A. Piras, D. Falconieri, D. Yalaoui- Guellal, M. F.  Elsebai, K. Madani and M. Chibane (2016). Chemical composition and in vitro antimicrobial, insecticidal and antioxidant activities of the essential oils of Mentha pulegium L. and Mentha rotundifolia (L.) Huds growing in Algeria. Ind. Crop. Prod., 88, 96-105. [Google Scholar]
  10. Celimene, C.C., J.A. Micales, L. Ferge and R.A. Young (1999). Efficacy of pinosylvins against white-rot and brown-rot fungi. Holzforschung, 53, 491-497. [Google Scholar]
  11. Champion, R. (1997). Identifier les champignons transmis par les semences, INRA, Paris, 398 p. [Google Scholar]
  12. Clevenger, J. F. (1928). Apparatus for the determination of volatile oil. J. Am. Pharm. Assoc., 17, 346-341. [Google Scholar]
  13. Dehghanpour-Farashah, S. and P. Taheril (2016). Antifungal and antiaflatoxigenic effects of Mentha longifolia essential oil against Aspergillus flavus. International Journal of New Technology and Research (IJNTR), 2 (9), 30-39. [Google Scholar]
  14. Derwich, E., Z. Benziane, R. Taouil, O. Senhaji and M. Touzani (2010). Comparative Essential oil Composition of Leaves of Mentha rotundifolia and Mentha pulegium a Traditional Herbal Medicine in Morocco.  Am. Eurasian J. Sustain. Agric., 4(1),  47-54. [Google Scholar]
  15. Djeugap, F. J., D. A. Fontem and A. L. Tapondjou (2009). Évaluation des milieux de culture pour la croissance de Phytophthora infestans. Cameroon Biosci. Proc., 15, 85-92. [Google Scholar]
  16. Dorman, H. J. D. and S. G.  Deans (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J. Appl. Microbiol., 88 (2), 308–316. [Google Scholar]
  17. El Ajjouri, M. (2013). Etude de la composition chimique et de l’efficacité des huiles essentielles de quelques espèces du genre Thymus dans la préservation du bois contre les champignons lignivores. Thèse de Doctorat. Université Mohammed V – Agdal, Rabat. Faculté des Sciences, Spécialité : Sciences du Bois, 181 p. [Google Scholar]
  18. El Arch, M., B. Satrani, A. Farah, L. Bennani, D. Briky, M. Fechtel, M. Blaghen and M. Talbi (2003). Chemical composition and antimicrobial and insecticidal activities of the essential oil in Mentha rotundifolia from Morocco. Acta Bot. Gallica, 150 (3),  267–274. [Google Scholar]
  19. Farag, R. S., Z. Y. Daw, F. M. Hewedi and G. S. A. El-Baroly (1989). Antimicrobial activity of some Egyptian spice essential oils. J. Food Prot., 52, 665-667. [Google Scholar]
  20. Farhat, A. (2010). Vapo-Diffusion assistée par Micro-ondes : Conception, Optimisation et Application. Thèse de Doctorat. Université d’Avignon et des Pays de Vaucluse et L’Ecole Nationale d’Ingénieurs de Gabès Marseille. Spécialité : Sciences des Procédés, Sciences des Aliments, 136 p. [Google Scholar]
  21. Giray, E. S., S. Kirici, D. A.  Kayab, M. Türk, Ö. Sönmez and M. Inan (2008). Comparing the effect of sub-critical water extraction with conventional extraction methods on the chemical composition of Lavandula stoechas. Talanta, 74, 930–935. [Google Scholar]
  22. Gulluce, M., F. Sahin, M. Sokmen, H. Ozer, D. Dafarera, A. Sokmen, M. Polissiou, A. Adiguzel and H. Ozkan  (2007). Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food Chem., 103(4), 1449-1546. [Google Scholar]
  23. Hajlaoui, H., N. Trabelsi, E. Noumi, M. Snoussi, H. Fallah, R. Ksouri and A. Bakhrouf (2009). Biological activities of the essential oils and methanol extract of tow cultivated mint species (Mentha longifolia and Mentha pulegium) used in the Tunisian folkloric medicine. World J. Microbiol. Biotech., 25, 2227–2238. [Google Scholar]
  24. Hussain, A. I., F. Anwar, S. P. Nigam, M. Ashraf and A. H. Gilani (2010). Seasonal variation in content, chemical composition and antimicrobial and cytotoxic activities of essential oils from four Mentha species. J. Sci. Food Agr., 90(11), 1827-1836. [Google Scholar]
  25. Knowles, J. R., S. Roller, D. B.  Murray and A. S. Naidu (2005). Antimicrobial action of carvacrol at different stages of dual-species biofilm development by Staphylococcus aureus and Salmonella enterica Serovar Typhimurium. Appl. Environ. Microbiol., 71, 797-803. [Google Scholar]
  26. Kotan, R.,  F. Dadasogl, K. Karagoz, A. Cakir, H. Ozer, S. Kordalia et al., (2013). Antibacterial activity of the essential oil and extracts of Satureja hortensis against plant pathogenic bacteria and their potential use as seed disinfectants. Sci. Hortic., 153, 34–41. [Google Scholar]
  27. Labiod, R., S. Aouadi and N. Bouhaddouda (2015). Chemical composition and antifungal activity of essential oil from Satureja calamintha nepeta against phytopathogens fungi. Int J Pharm Pharm Sci., 7 (7), 208-211. [Google Scholar]
  28. Lehbab, A., (2013). Etude phytochimique et évaluation de l'activité antimicrobienne des extraits de Mentha rotundifolia L. (Domrane) de la région de Tlemcen Composition chimique des huiles essentielles. Mémoire de Master en Biologie, Université Abou Bekr Belkaid, Tlemcen, 57p. [Google Scholar]
  29. Lingk, W., (1991). Health risk evaluation of pesticide contamination in drinking water. Gesunde Pflanz 43, 21–5. [Google Scholar]
  30. Lopez-Malo, A., S. M. Alzamora and E. Palou (2005). Aspergillus flavus growth in the presence of chemical preservatives and naturally occurring antimicrobial compounds. Int. J. Food Microbiol., 99, 119-128. [Google Scholar]
  31. Lorenzo, D., D. Paz, E. Dellacassa, P. Davies, R.  Vila and S. Canigueral  (2002). Essential oils of Mentha pulegium and Mentha rotundifolia from Uruguay. Bras. Arch. Boil. Technol., 45 (4), 519–524. [Google Scholar]
  32. Mann C.M., Cox S.D. et Markham J.L., (2000). The outer membrane of Pseudomonas aeruginosa NCTC 6749 contributes to the essential oil of Melaleuca alternifolia (tea tree oil), Letters in appl. Microbiol 30, 294-297. [Google Scholar]
  33. McKay, D. L. and J. B. Blumberg (2006). A review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.), Phytotherapy Res., 20, 519- 530. [Google Scholar]
  34. Mehani, M., L. Segni, V. Terzi, C. Morcia, R. Ghizzoni, M. B. Goudjil and S. E. Bencheikh (2015). Antibacterial, antifungal activity and chemical composition study of essential oil of Mentha pepirita from the south Algerian. Der Pharma Chemica, 7(12), 382-387. [Google Scholar]
  35. Messiaen, C. M., D. Blancard, F. Rouxel and R.  Lfon (1993). Les maladies des plantes maraichères. 3ème éd. INRA, Paris. [Google Scholar]
  36. Mimica-Dukić, N. and B.  Božin (2008). Mentha L. species (Lamiaceae) as promising sources of bioactive secondary metabolites. Curr. Pharm. Des., 14(29), 3141-3150. [Google Scholar]
  37. Mkaddem, M., J. Bouajila, M. Ennajar, A. Lebrihi, F. Mathieu and M.  Romdhane (2009). Chemical composition and antimicrobial and antioxidant activities of Mentha (longifolia L. and viridis) essential oils. J. Food Sci., 74, 358–363. [Google Scholar]
  38. Moghaddam, M., M. Pourbaige, H. K. Tabar, N. Farhadi and S. M. Ahmadi Hosseini (2013). Composition and Antifungal Activity of Peppermint (Mentha piperita) Essential Oil from Iran. TEOP 16 (4),  506 – 512. [Google Scholar]
  39. Moghtader, M., (2013). In vitro antifungal effects of the essential oil of Mentha piperita L. and its comparison with synthetic menthol on Aspergillus niger. Afr. J. Plant Sci., 7(11), 521-527. [Google Scholar]
  40. Nagell, A. and F. W. Hefendehl (1974). Composition of the essential oil of Mentha rotundifolia. Planta Med., 26, 1–8. [Google Scholar]
  41. Navi, S.S., R. Bandyopadhyay, A. J. Hall and P. J. Bramel-Cox (1999). A pictorial guide for the identification of mold fungi on Sorghum grain. International Crops Research Institute for the Semi-Arid Tropics. Natural Resources Institute. Bulletin N° 59, 118 p. [Google Scholar]
  42. Norsati, S., S. A. E. Hosseini, A. Sarpeleh, M. SoflaeiShahrbabak and Y. SoflaeiShahrbabak (2011). Antifungal Activity of Spearmint (Mentha Spicata L.) Essential oil on Fusarium oxysporum f. sp. radicis-cucumerinum the causal agent of stem and crown rot of greenhouse cucumber in Yazd, Iran. International Conference on Environmental and Agriculture Engineering, IPCBEE, 15, 52-56. [Google Scholar]
  43. Oumzil, H., S. Ghoulami, M. Rhajaoui, A. Ilidrissi, S. Fkih-Tetouani, M. Faid and A.  Benjouad (2002). Antibacterial and antifungal activity of essential oils of Mentha suaveolens. Phytother. Res., 16, 727–731. [Google Scholar]
  44. Ozen, T., I. Demirtas and H. Aksit (2011). Determination of antioxidant activities of various extracts and essential oil compositions of Thymus praecox subsp. skorpilii var. skorpilii. Food Chem., 124,  58–64.  [Google Scholar]
  45. Park, Y. J., T. B. Baskar, S. K. Yeo, M. V. Arasu,  N. A. Al‑Dhabi, S. S. Lim and S. U.  Park (2016). Composition of volatile compounds and in vitro antimicrobial activity of nine Mentha spp. SpringerPlus, 5, 1628. [Google Scholar]
  46. Peixoto, I. T. A, V. F. Furlanetti, P. C. Anibal, M. C. T. Duarte and J. F. Hofling (2009). Potential pharmacological and toxicological basis of the essential oil from Mentha spp. Rev. Ciěnc. Farm. Basica Apl., 30(3), 235‐239. [Google Scholar]
  47. Pino, J.A., A. Rosado and V. Fuentes (1999). Chemical composition of the leaf oil of Mentha rotundifolia (L.) Hudson from Cuba. J. Essent. Oil Res., 11, 241–242. [Google Scholar]
  48. Pitt, J. I. and A. D.  Hocking (1997). Fungi and food spoilage. First edition 1985 published by Academic Press, Sydney. Blackie Academic & Professional. [Google Scholar]
  49. Pradhanang, P.M., M. T. Momol, S. M. Olson and J. B.  Jones (2003). Effects of plant essential oils on Ralstonia solanacearum population density and bacterial wilt incidence in tomato. Plant Dis., 87, 423–427. [Google Scholar]
  50. Proestos, C., I. S. Boziaris, G. J. E. Nychas and M.  Komaitis (2006). Analysis of flavonoids and phenolic acids in Greek aromatic plants: investigation of their antioxidant capacity and antimicrobial activity. Food Chem., 95, 664–671. [Google Scholar]
  51. Raya, M.D.P., M. P. Utrilla, M. C. Navarro and J. Jiminez (1990). CNS activity of Mentha rotundifolia and Mentha longifolia essential oil in mice and rats. Phytotherapy Res., 4, 232–234. [Google Scholar]
  52. Reddy, D.N., A. J. Al-Rajab, M. Sharma, M. M. Moses, G. R. Reddy and M.  Albratty (2017). Chemical constituents, in vitro antibacterial and antifungal activity of Mentha piperita L. (peppermint) essential oils. Journal of King Saud University–Science.  [Google Scholar]
  53. Remmal, A., A. Tantaoui-Eiaraki, T. Bouchikhi, M. Rhayour and K. Ettayebi (1993). Improved method for determination of antimicrobial activity of essential oils in agar medium. J. Ess. Oil. Res., 5, 179-184. [Google Scholar]
  54. Riahi, L., M. El ferchichi, H. Ghazghazi, J. Jebali, S. Ziadi, C. Aouadhi, H. Chograni, Y. Zaouali, N. Zoghlami and A. Mliki (2013). Phytochemistry, antioxidant and antimicrobial activities of the essential oils of Mentha rotundifolia L. in Tunisia. Ind. Crop Prod., 49, 883-889. [Google Scholar]
  55. Teixeira, B., A. Marques, C. Ramos, I. Batista, C. Serrano, O. Matos, N. R. Neng, J. M. F. Nogueira, J. A. Saraiva and M. L.  Nunes (2012). European pennyroyal (Mentha pulegium) from Portugal: chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Ind. Crop  Prod., 36, 81–87. [Google Scholar]
  56. Tian, J., X. Ban, H. Zeng, J. He, B. Huang and Y. Wang (2011). Chemical composition and antifungal activity of essential oil from Cicuta virosa L. var. latisecta Celak. Int. J. Food Microbiol., 145, 464–70. [Google Scholar]
  57. Viennot-Bourgin, G. (1980). Systématique des Champignons Parasites des Plantes (5e ed). Centre de Documentation Universitaire et SEDES réunis : Place de la Sorbonne, Paris Ve. [Google Scholar]
  58. Watanabe ,T. (2002). Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species, 2nd Edition, 486 p. [Google Scholar]
  59. Zhu, J., Z. Zhang and Z. Yang (2001). General research methods on pathogen of potato late blight (Phytophthora infestans). J. Agric. Sci., 24, 112- 114. [Google Scholar]
Meta
Vol. 4 (1)
Download
Metric
History
Related

Volume 4 Issue 1

March 2020

All Articles
Copyright © 2012 - 2024 International Journal of Innovative Approaches in Agricultural Research.
Pen Academic Publishing is a trademark of THDSoft.
This work is licensed under a Creative Commons Attribution 4.0 International License. CC BY-NC-ND
Electronic Journal Management System. This software was developed in Türkiye. ejournal.gen.tr
Pen Academic Publishing General Publication Policy: