Production of <em>Trichoderma harzianum</em> (127a and 127b) spores by Fermentation (LF and SSF)

Almı Hiba, Dehimat Laid, Zaki Omrane, Ahmed Sabri & Philipe Thonart

doi:https://doi.org/10.29329/ijiaar.2019.206.3

Original article | Open Access
International Journal of Innovative Approaches in Agricultural Research 2019, Vol. 3(3) 376-384

Production of Trichoderma harzianum (127a and 127b) spores by Fermentation (LF and SSF)

Almı Hiba, Dehimat Laid, Zaki Omrane, Ahmed Sabri & Philipe Thonart

pp. 376 - 384 | DOI: https://doi.org/10.29329/ijiaar.2019.206.3

Published online: September 30, 2019 | Number of Views: 281 | Number of Download: 903

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Abstract

In order to produce a bio fungicide, two strains of Trichoderma harzianum (127a and 127b) were used.The results revealed that the mycelium multiplication of Trichoderma harzianum (127a and 127b) strains in liquid fermentation yielded a good mycelial mass (106 cells / ml), or the optimal conditions are a pH 5, a temperature of 30° C, and a medium of culture made of Malt extract and yeast extract. However, the more or less unfavorable conditions for the development of mycelium (FMS) favored the appearance of the spore form of our two antagonistic strains, which resulted in a smooth greenish powder at the end of the fermentation, on a solid medium.

Keywords: Trichoderma harzianum, liquid fermentation, solid-state fermentation, Wheat Bran.

How to Cite this Article

APA 6th edition
Hiba, A., Laid, D., Omrane, Z., Sabri, A. & Thonart, P. (2019). Production of Trichoderma harzianum (127a and 127b) spores by Fermentation (LF and SSF) . International Journal of Innovative Approaches in Agricultural Research, 3(3), 376-384. doi: 10.29329/ijiaar.2019.206.3

Harvard
Hiba, A., Laid, D., Omrane, Z., Sabri, A. and Thonart, P. (2019). Production of Trichoderma harzianum (127a and 127b) spores by Fermentation (LF and SSF) . International Journal of Innovative Approaches in Agricultural Research, 3(3), pp. 376-384.

Chicago 16th edition
Hiba, Almi, Dehimat Laid, Zaki Omrane, Ahmed Sabri and Philipe Thonart (2019). "Production of Trichoderma harzianum (127a and 127b) spores by Fermentation (LF and SSF) ". International Journal of Innovative Approaches in Agricultural Research 3 (3):376-384. doi:10.29329/ijiaar.2019.206.3.

References

Andrews, J.H. (1992). Biological control in the phyllosphere. Annu. Rev. Phytopathol., 30, 603-635. [Google Scholar]
Gveroska, B. and J. Ziberoski (2011). Trichoderma harzianum as a biocontrol agent against Alternaria alternata on tobacco. ATI - Applied Technologies & Innovations. 7 (2), 67-76. [Google Scholar]
Cook J.R. and K.F. Baker (1983). The Nature and Practice of Biological Control of Plant Pathogens. American Phytopathological Society, St. Paul. [Google Scholar]
Harman, G. E., C. R. Howell, A. Viterbo, I. Chet and M. Lorito (2004). Trichoderma species – opportunistic, avirulent plant symbionts. Nat. Rev. Microbiol., 2, 43–56. [Google Scholar]
Klein, D and D.E. Eveleigh (1998). Ecology of Trichoderma . In: Kubicek CP, Harman GE, Eds. Trichodermaand Gliocladium. Vol. 1. Basic Biology, Taxonomy and Genetics. London: Taylor and Francis Ltd.pp. 57–74. [Google Scholar]
Monte, E. and A. Llobell (2003). Trichoderma in organic agriculture. Proc. V World Avocado Congress (Actas V Congreso Mundial del Aguacate) 2003. pp. 725-733. [Google Scholar]
Niranjana, S.R., S. Lalitha and P. Hariprasad (2009). Mass multiplication and formulations of biocontrol agents for use against Fusarium wilt of pigeon pea through seed treatment. Intern. J. Pest Manag., 55(4), 317–324. [Google Scholar]
Papavizas, G.C. and R.D. Lumsader (1982). Improved medium for isolation of Trichoderma spp. from soil. Plant Dis., 66, 1019-1020. [Google Scholar]
Pedreschi, F. and J. M. Aguilera (1997). Viability of dry Trichoderma harzianum spores under storage, Bioprocess Eng., 177-183. [Google Scholar]
Cavalcante, R.S., H. L.S. Lima, G. A. S. Pinto, C. A. T. Gava and S. Rodrigues (2008). Effect of Moisture on Trichoderma Conidia Production on Corn and Wheat Bran by Solid State Fermentation. Food Bioproc. Tech., 1(1), 100–104. [Google Scholar]
Tarus, P., S. Chhabra, C.C. Lang'at-Thoruwa, A. Wanyonyi (2003) Afr. J. Health Sci. 2003, in press. [Google Scholar]
Tewari L. and Bhanu C. 2004. Evaluation of agro-industrial wastes for conidia bases incoulum production of bio-control agent: Trichoderma harzanium. J. Sci. Ind. Res., 6, 807–812. [Google Scholar]
Warcup J. H. (1955). On the origin of colonies of fungi developing on soil dilution plates. Trans. Br. Mycol. Soc., 38, 298-301. [Google Scholar]
Zuriash Mamo and Tesfaye Alemu (2012). Evaluation and optimization of agro- industrial wastes for conidial production of Trichoderma isolates under solid state fermentation. J. Appl. Biosci., 54, 3870– 3879. [Google Scholar]

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