Soil Microorganisms Quantitative Dynamic Characterizing the Overall Biological State in Rhizosphere of Tobacco and Tomato Plants Infected by Broomrape

Tsveta Hristeva, Iliya Denev & Radka Bojinova

doi:https://doi.org/10.29329/ijiaar.2019.206.7

Original article | Open Access
International Journal of Innovative Approaches in Agricultural Research 2019, Vol. 3(3) 411-419

Soil Microorganisms Quantitative Dynamic Characterizing the Overall Biological State in Rhizosphere of Tobacco and Tomato Plants Infected by Broomrape

Tsveta Hristeva, Iliya Denev & Radka Bojinova

pp. 411 - 419 | DOI: https://doi.org/10.29329/ijiaar.2019.206.7

Published online: September 30, 2019 | Number of Views: 137 | Number of Download: 677

Download PDF

Abstract

The quantitative dynamics of soil microorganisms in the rhizosphere of tobacco and tomato plants infected by broomrape /Phelipanche ramosa L./ were investigated. Parallel pot experiments with the two hosts of the parasite - Oriental type of tobacco and tomato were presented. The variants were: by introducing contamination into the soil - seeds by broomrape and control pots - without the seeds by broomrape. Experiments were carried out under controlled conditions. Soil samples for microbiological analyzes were taken from the rhizosphere, in dynamics. The microbiological analyses were executed according to Koh’s, in three replications (MPN/g a.d.s), with confidence level 0.05. The surveyed set of soil microorganisms has been comprised two indicators groups characterizing the overall biological state of the microbial communities: Autochthonous (on soil extract agar) and Oligotrophic (on diluted soil extract agar). Diversity indexes Shannon (H) and Simpson (D) and the distribution evenness (EH) in the microbial communities were determined. A statistical analysis has been made. The obtained results showed that the quantities of the two groups of microorganisms begun to change visibly around and after 20th day, compared to the amount at the rhizosphere of uninfected by broomrape plants. There was an increase at the population density of autochthonous microorganisms between 20 and 60 days and reduced significantly after this period. The trend was reversed at oligotrophic microorganisms, but the quantities were higher throughout the study period. These dynamics coincide with the phases of broomrape development - germination, formation of a haustorium (about 10-20th day) and attachment to the root of the host (up to about 40-60 days) and the above-ground phases (80-100 days). The analysis showed that microbial communities formed in rhizospheres in the presence of a broomrape had been an oligotrophic character. The relative share of oligotrophic microorganisms in these microbial communities was over 50% at both host rhizospheres.

Keywords: : branched broomrape; tobacco, tomato; rhizosphere, autochthonous microorganisms; oligotrophic microorganisms

How to Cite this Article

APA 6th edition
Hristeva, T., Denev, I. & Bojinova, R. (2019). Soil Microorganisms Quantitative Dynamic Characterizing the Overall Biological State in Rhizosphere of Tobacco and Tomato Plants Infected by Broomrape . International Journal of Innovative Approaches in Agricultural Research, 3(3), 411-419. doi: 10.29329/ijiaar.2019.206.7

Harvard
Hristeva, T., Denev, I. and Bojinova, R. (2019). Soil Microorganisms Quantitative Dynamic Characterizing the Overall Biological State in Rhizosphere of Tobacco and Tomato Plants Infected by Broomrape . International Journal of Innovative Approaches in Agricultural Research, 3(3), pp. 411-419.

Chicago 16th edition
Hristeva, Tsveta, Iliya Denev and Radka Bojinova (2019). "Soil Microorganisms Quantitative Dynamic Characterizing the Overall Biological State in Rhizosphere of Tobacco and Tomato Plants Infected by Broomrape ". International Journal of Innovative Approaches in Agricultural Research 3 (3):411-419. doi:10.29329/ijiaar.2019.206.7.

References

Amsellem, Z., S. Barghouthi, B. Cohen, Y. Goldwasser, J. Gressel, L. Hornok, Z. Kerenyi, Y. Kleifeld, O. Klein and J. Kroschel (2001). Recent advances in the biocontrol of Orobanche (broomrape) species, J. BioControl, 46, 2, 211-228. [Google Scholar]
Beals, M., L. Gross and S. Harrell (1999), Diversity Indices: Simpson’s D and E. [Google Scholar]
Burns, R. G., J. L. De Forest, J. Marxsenc, R. L. Sinsabaughd, M. R. Strombergere, M. D. Wallensteinf, M. N. Weintraubg and A. Zoppinih (2013). Soil enzymes in a changing environment: Current knowledge and future directions. Soil Biol. Biothech., 59, 216-234. [Google Scholar]
Cavar, S, B. Zwanenburg and P. Tarkowski (2015). Strigolactones: occurrence, structure, and biological activity in the rhizosphere. Phytochem Rev., 14, 691–711. [Google Scholar]
Chen, J., Q. H. Xue, C. S. McErlean, J. H. Zhi, Y. Q. Ma, X. T. Jia, M. Zhang and X. X.Ye (2016). Biocontrol potential of the antagonistic microorganism Streptomyces enissocaesilis against Orobanche cumana. J. BioControl, 1, 1-11 [Google Scholar]
Dennis, P. G., A. J. Miller and P. R. Hirsch (2010). Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities? FEMS Microbiol. Ecol., 72, 313–327. [Google Scholar]
Fernandez-Aparicio, M., K. Yoneyama and D. Rubiales (2011). The role of strigolactones in host specificity of Orobanche and Phelipanche seed germination. Seed Sci. Res., 21, 55–61. [Google Scholar]
Hristeva, Ts., T. Dekalska and I. Denev (2013). Structural and functional biodiversity of microbial communities in rhizospheres of plants infected with broomrapes (Orobanchaceae). J. Biotech. Biotech. Equip., 27 (5). [Google Scholar]
Hristeva, Ts. and I. Denev. (2017). Changes at the Rhizosphere Microbiota of the Sunflower - Orobanche cumana Wall Pathosystem. Int. J. Curr. Microbiol. App. Sci., 6(1), 733-746. [Google Scholar]
Joel, D.M., J. Hershenhorn, H. Eizenberg, R. Aly, G. Ejeta, P.J. Rich, J. K. Ransom, J. Sauerborn and D. Rubiales (2007). Biology and management of weedy root parasites. Hortic. Rev., 33, 267–349. [Google Scholar]
Morris, E. K., T. Caruso, F. Buscot, M. Fischer, Ch. Hancock, T.Maier, T. Meiners, C. Müller, E. Obermaier, D. Prati, St. Socher, I. Sonnemann, N. Wäschke, T. Wubet, S. Wurst and M. Rillig (2014). Choosing and using diversity indices: insights for ecological applications from the German Biodiversity Exploratories Ecol Evol., 4(18), 3514–3524. [Google Scholar]
Koleshko, O. I. (1991). Ecology of soil microorganisms Handbook, University Press., Minsk: 19-136. [Google Scholar]
Longo, A. M. G., A. Lo Monaco and G. Mauromicale (2010). The effect of Phelipanche ramosa infection on the quality of tomato fruit. Weed Res., 50, 58–66. [Google Scholar]
Magurran, A. E. (2004). Meausuring Biological Diversity. Blackwell. [Google Scholar]
Parker, C. (2009). Observations on the current status of Orobanche and Striga problems worldwide. Pest Manag. Sci., 65, 453–459. [Google Scholar]
Panchenko, A.Y. (1975). Accelerated method of assessing breeding materials for resistance in broomrape. Her. Agric. Sci., 2, 107- 115. [Google Scholar]
Ranjan, A., Y. Ichihashi, M. Farhi, K. Zumstein, B. Townsley, T. David, R. Vera, J. M. García, M. J. Pozo and J. A. López-Ráez (2016). Expression of molecular markers associated to defense signaling pathways and strigolactone biosynthesis during the early interaction tomato Phelipanche ramosa. Physiol. Mol. Plant Pathol., 94, 100–107. [Google Scholar]
Sauerborn, J., D. Muller-Stover, J. Hershenhorn (2007). The role of biological control in managing parasitic weeds. Crop Prot., 26, 246–254. [Google Scholar]
Zermane, N., T. Souissi, J. Kroschel and R. Sikora (2007). Biocontrol of broomrape (Orobanche crenata Forsk. and Orobanche foetida Poir.) by Pseudomonas fluorescens isolate Bf7-9 from faba bean rhizosphere. J. Biocontrol Sci.Tech., 17 (5). 483-497. [Google Scholar]
Zonno, M. C. and M. Vurro (2002). Inhibition of germination of Orobanche ramosa seeds by Fusarium toxins. Phytoparasitica, 30, 519-524. [Google Scholar]
Vurro, M., A. Boari, A. Evidente, A. Andolfi and N. Zermane (2009). Natural metabolites for parasitic weed management. Pest Manag. Sci., 65, 566-571. [Google Scholar]
Werner D., (2001). The organic signals between plants and microorganisms. In: Pinton R., Varanini Z., Nannipieri (eds.). Rhizosphere biochemistry and organic substances in the soil-plant interface. Marcel Dekker, New York: 197-222. [Google Scholar]
Wegmann K. (1986). Biochemistry of osmoregulation and possible biochemical reasons of resistance against Orobanche. In: S. J. ter Borg (ed.) roc. of a Workshop in Wageningen, The Netherlands: 42-50. [Google Scholar]

Volume 3 Issue 3

September 2019

All Articles