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International Journal of Innovative Approaches in Agricultural Research 2024, Vol 8(2) 62-72

Investigation of the Effectiveness of Inoculation Methods of Macrophomina Phaseolina, the Causative Agent of Coal Rot Disease, in Soybean

Sema Sahbaz • Fatih Mehmet Tok • Yaşar Akışcan
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Article Type
Research article
Publication Date
June 30, 2024
Pages
62-72
DOI
10.29329/ijiaar.2024.1049.1

Abstract

Although soybean is affected by many fungal diseases from seed to harvest, charcoal rot caused by Macrophomina phaseolina is one of the most important diseases of soybean. It is important to grow resistant varieties because microsclerots can remain alive in the soil for many years, and there is currently no effective chemical control. Currently, many inoculation methods are used to determine the resistance of soybean plants to M. phaseolina; however, their effectiveness under field conditions has not been fully determined. In this study, soybean plants were inoculated with M. phaseolina during the flowering period using agar-disc, toothpick, and microsclerot injection methods, and the efficiency of these methods was investigated. In the disease observations made close to harvest, the disease index value was 3.3 in the agar disk method, 1.9 in the toothpick method and 1.3 in the microsclerot method. The results of the study showed that the symptoms in all methods were similar to those in field conditions, the agent could be transmitted by seeds and had an effect on seed quality, and the agar-disc method was the most successful method among the methods used to inoculate soybean plants with M. phaseolina under field conditions.

Keywords: Soybean Disease Macrophomina Charcoal rot Resistance
Cite this article
Sahbaz, S., Tok, F. M., & Akışcan, Y. (2024). Investigation of the Effectiveness of Inoculation Methods of <em>Macrophomina Phaseolina</em>, the Causative Agent of Coal Rot Disease, in Soybean. International Journal of Innovative Approaches in Agricultural Research, 8(2), 62-72. https://doi.org/10.29329/ijiaar.2024.1049.1

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