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International Journal of Innovative Approaches in Agricultural Research 2023, Vol 7(4) 514-534

Determination of the Bacterial Community in Soils Associated with Rare Wild Leguminous Species Cicer Montbretii Jaub. & Spach and Lupinus albus L. in Strandzha Natural Park

Mariana Petkova • Maryia Sabeva • Nurettin Tahsin
Cite this article
Article Type
Research article
Publication Date
December 31, 2023
Pages
514-534
DOI
10.29329/ijiaar.2023.630.12

Abstract

The soil formation in Strandzha Mountain is influenced by the particular combination of the climate's unique forest tree vegetation, the extraordinary variety of root and soil-forming rocks, the hilly low-mountainous relief with significant fragmentation, a densely located hydrographic network with short slopes and dominant exposures. The diversity of soil microorganisms is crucial for plant growth and development and it makes it possible to understand in detail the plant-microbial interactions. The objectives of this study were to determine soil bacteria associated with rare wild leguminous species Cicer montbretii Jaub. & Spach (Constantinople chickpeas) and Lupinus albus L. (white lupinus) in Strandzha National Park. A new locality of Cicer montbretii Jaub was marked nearby village of Brodilovo. L. albus was found in saline-alkaline soil (A1) and yellow earth podzolic soils (A2) around the village of Brodilovo and the Great Pazvlak area. C. montbretii was found to grow on cinnamon forest soils (B1) and siliceous red soil (B2). A study was conducted by physio-chemical analyses and by assessing 16S rDNA metagenomics technique used to generate a total of 126,837 reads from the samples.  The most significant number of observed species 2249 was found in soils saline-alkaline soil (B1) soil. According to that result, the higher diversity indices were calculated in the also in B1 soil. The α-diversity analysis reported yielded similar Shannon indices ranging from 8,322 in B2 to 9,337 in B1. The analyses revealed that B2 yellow earth podzolic soil, unique for Strandzha, has the largest composition with Proteobacteria 44% and the lowest in Actinobacteria 20%. Opposite, in A1 saline-alkaline soil have the richest composition of Actinobacteria 52% and the poorest in Proteobacteria 23%. C. montbretii was found in neutral A2 and B2 soils, while L. ablus prefer acidic A1 and B1 soils. The determination of the microbiological status of the soils associated with Constantinople chickpeas and white lupinus and the annual monitoring of the species in Strandzha Park will determine the methods for the most effective maintenance and storage outside their habitats.

Keywords: NGS Cicer montbretii Jaub. & Spach Lupinus ablbus L. expedition Strandzha Nature Park
Cite this article
Petkova, M., Sabeva, M., & Tahsin, N. (2023). Determination of the Bacterial Community in Soils Associated with Rare Wild Leguminous Species <em>Cicer Montbretii</em> Jaub. &amp; Spach and <em>Lupinus albus</em> L. in Strandzha Natural Park. International Journal of Innovative Approaches in Agricultural Research, 7(4), 514-534. https://doi.org/10.29329/ijiaar.2023.630.12

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