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International Journal of Innovative Approaches in Agricultural Research 2022, Vol 6(3) 175-183

The Effect of Oxidized Fish Oil on Blood Parameters and Liver Histology of Nile Tilapia (Oreochromis niloticus)

Sana Yagoub Abdallah Tahir • Aysun Kop
Cite this article
Article Type
Research article
Publication Date
September 30, 2022
Pages
175-183
DOI
10.29329/ijiaar.2022.475.1

Abstract

Fish oil is a virtually unique source of natural LC omega-3 fatty acids EPA, DPA, and DHA, as an important source of lipid in aquafeeds. However during processing and storage, fish oil is prone to oxidation. The oxidized oil has many negative effects on the growth and health of farmed fish, therefore this study was conducted to investigate the effect of oxidized anchovy oil on blood parameters and liver histology of Nile tilapia (Oreochromis niloticus). Nile tilapia fingerlings with an initial average body weight of (15.07±0.58 g) were stocked in nine fiberglass tanks (with a tank capacity of 120 liters) in three groups in triplicates at a density of 50 fish/tank. Fish were fed with three test diets containing 35% crude protein and 8% crude fat (POV: 4.85, 50.10, and 100.80 meq/kg-1) twice a day at a feeding rate of 3% of their body weight per day for 12 weeks. During the experiment fish blood parameters analysis, and liver histology tests were performed on 3 fish taken randomly from each tank at the beginning of the experiment and monthly. The results of blood chemistry analysis showed better results in the group fed with feed that contained fresh oil (A) compared to groups fed with feeds that contained oxidized oils (B and C), but no significant differences (P > 0.05) between feed groups. Whereas the results of serum biochemical analysis showed significant differences (P > 0.05) between feed groups during the experiment period, oxidized fish oil increased serum ALT, AST activity, and TCHO level (P < 0.05), and decreased ALP activity. The liver histology test showed the best results in group A compared to oxidized oil groups (B, C), oxidized fish oil caused damage to the liver structure, and hepatocyte nuclear migration and lipid droplets were observed in liver samples from the oxidized oil groups. In conclusion, the present study demonstrates that oxidized fish oil may have a negative effect on the liver histology and blood parameters of Nile tilapia fingerlings.

Keywords: Oil Oxidation Blood Parameters Liver Histology Nile Tilapia
Cite this article
Tahir, S. Y. A. & Kop, A. (2022). The Effect of Oxidized Fish Oil on Blood Parameters and Liver Histology of Nile Tilapia (<em>Oreochromis niloticus</em>). International Journal of Innovative Approaches in Agricultural Research, 6(3), 175-183. https://doi.org/10.29329/ijiaar.2022.475.1

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