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International Journal of Innovative Approaches in Agricultural Research 2026, Vol 10(1) 1-13

Determining the Efficiency of ISSR Markers among Wild Prunus spinosa Genotypes

Ödül Efsane Ayçiçek • Hayat Topçu • Behiye Banu Bilgen
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Article Type
Research article
Publication Date
July 03, 2026
Pages
1-13
DOI
10.29329/ijiaar.2026.1423.16

Abstract

Prunus spinosa L., commonly known as ‘Çakal Eriği’ or ‘Güvem’ in Türkiye, is a wild plum species that naturally occurs across many regions of the country, particularly in the Thrace region. Despite its ability to thrive in harsh environmental conditions and its wide habitat range, this species is not cultivated commercially, and scientific studies involving P. spinosa remain limited. In this study, the effectiveness of ISSR (Inter Simple Sequence Repeat) markers was evaluated using seven previously unstudied P. spinosa genotypes collected from the Thrace region. Of the 27 ISSR primers used, 17 were successfully amplified, yielding a total of 244 bands. Among these, 216 bands were polymorphic, resulting in an average polymorphism rate of 86.49%. The number of bands per primer ranged from 5 (ISSR825) to 24 (ISSR823 and ISSR844), with an average of 14.8 bands per primer. The amplified fragment sizes ranged between 290 and 2100 base pairs. The polymorphism information content (PIC) values varied from 0.313 (ISSR843) to 0.424 (ISSR825), with an average PIC value of 0.365. Genetic similarity coefficients based on Jaccard’s similarity index ranged between 0.627 and 0.816, with a mean value of 0.745. These findings demonstrate that ISSR markers are effective molecular tools for assessing genetic diversity among P. spinosa genotypes. In particular, primers ISSR825 (0.424), ISSR841 (0.397), and ISSR808 (0.392) exhibited high levels of discrimination, making them especially useful for detecting genetic differences. The results indicate that ISSR markers can be reliably used to investigate genetic diversity in previously uncharacterized P. spinosa genotypes, with ISSR825, ISSR841 and ISSR808 primers standing out in particular due to their high discriminatory potential.

Keywords: Genetic Diversity ISSR Prunus spinosa L. Wild Plum
Cite this article
Ayçiçek, Ö. E., Topçu, H., & Bilgen, B. B. (2026). Determining the Efficiency of ISSR Markers among Wild Prunus spinosa Genotypes. International Journal of Innovative Approaches in Agricultural Research, 10(1), 1-13. https://doi.org/10.29329/ijiaar.2026.1423.16

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