Pen Academic Publishing   |  e-ISSN: 2602-4772

Original article | International Journal of Innovative Approaches in Agricultural Research 2019, Vol. 3(1) 112-122

Chickpea Callus Histology Inoculated with Ascochyta Rabiei Blight Causal Agent Spores

Amina Kadırı, Zineb Faiza Boukhatem, Yamina Halfaoui & Zohra Ighilhariz

pp. 112 - 122   |  DOI: https://doi.org/10.29329/ijiaar.2019.188.11   |  Manu. Number: MANU-1807-25-0001

Published online: March 29, 2019  |   Number of Views: 14  |  Number of Download: 138


Abstract

The objective of this research was to study the interaction between Ascochyta rabiei as a pathogen and its host, established through in vitro tissue culture using as a pathosystem chickpea Cicer arietinum callus inoculated with Ascochyta rabiei spores. A resistant chickpea genotype INRA 199 and a local cultivar Zouaoui were used.

Murashige and Skoog, (1962) medium supplemented with 0.5 mg/l of Naphthalene Acetic Acid (NAA) and 1 mg/l of Benzyl Amino Purine (BAP) were used for the production of calluses used as a host. The histological study of calli inoculated with Ascocchyta rabiei spore suspension compared to the control showed two different reactions. In INRA199, the pathogen proliferation was slow and limited by the formation of an area where the cells accumulated phenolic compounds whereas in the cultivar Zouaoui the pathogen rapidly colonized the calli intercellular space and the number of formed pycnidia was high.

Keywords: Cicer arietinum, Pathosystem, In vitro culture, Chickpea blight


How to Cite this Article?

APA 6th edition
Kadiri, A., Boukhatem, Z.F., Halfaoui, Y. & Ighilhariz, Z. (2019). Chickpea Callus Histology Inoculated with Ascochyta Rabiei Blight Causal Agent Spores . International Journal of Innovative Approaches in Agricultural Research, 3(1), 112-122. doi: 10.29329/ijiaar.2019.188.11

Harvard
Kadiri, A., Boukhatem, Z., Halfaoui, Y. and Ighilhariz, Z. (2019). Chickpea Callus Histology Inoculated with Ascochyta Rabiei Blight Causal Agent Spores . International Journal of Innovative Approaches in Agricultural Research, 3(1), pp. 112-122.

Chicago 16th edition
Kadiri, Amina, Zineb Faiza Boukhatem, Yamina Halfaoui and Zohra Ighilhariz (2019). "Chickpea Callus Histology Inoculated with Ascochyta Rabiei Blight Causal Agent Spores ". International Journal of Innovative Approaches in Agricultural Research 3 (1):112-122. doi:10.29329/ijiaar.2019.188.11.

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