Chickpea Callus Histology Inoculated with <em>Ascochyta Rabiei </em>Blight Causal Agent Spores

Amina Kadırı, Zineb Faiza Boukhatem, Yamina Halfaoui & Zohra Ighilhariz

doi:https://doi.org/10.29329/ijiaar.2019.188.11

Original article | Open Access
International Journal of Innovative Approaches in Agricultural Research 2019, Vol. 3(1) 112-122

Chickpea Callus Histology Inoculated with Ascochyta Rabiei Blight Causal Agent Spores

Amina Kadırı, Zineb Faiza Boukhatem, Yamina Halfaoui & Zohra Ighilhariz

pp. 112 - 122 | DOI: https://doi.org/10.29329/ijiaar.2019.188.11

Published online: March 29, 2019 | Number of Views: 85 | Number of Download: 724

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Abstract

The objective of this research was to study the interaction between Ascochyta rabiei as a pathogen and its host, established through in vitro tissue culture using as a pathosystem chickpea Cicer arietinum callus inoculated with Ascochyta rabiei spores. A resistant chickpea genotype INRA 199 and a local cultivar “Zouaoui” were used.

Murashige and Skoog, (1962) medium supplemented with 0.5 mg/l of Naphthalene Acetic Acid (NAA) and 1 mg/l of Benzyl Amino Purine (BAP) were used for the production of calluses used as a host. The histological study of calli inoculated with Ascocchyta rabiei spore suspension compared to the control showed two different reactions. In INRA199, the pathogen proliferation was slow and limited by the formation of an area where the cells accumulated phenolic compounds whereas in the cultivar Zouaoui the pathogen rapidly colonized the calli intercellular space and the number of formed pycnidia was high.

Keywords: Cicer arietinum, Pathosystem, In vitro culture, Chickpea blight

How to Cite this Article

APA 6th edition
Kadiri, A., Boukhatem, Z.F., Halfaoui, Y. & Ighilhariz, Z. (2019). Chickpea Callus Histology Inoculated with Ascochyta Rabiei Blight Causal Agent Spores . International Journal of Innovative Approaches in Agricultural Research, 3(1), 112-122. doi: 10.29329/ijiaar.2019.188.11

Harvard
Kadiri, A., Boukhatem, Z., Halfaoui, Y. and Ighilhariz, Z. (2019). Chickpea Callus Histology Inoculated with Ascochyta Rabiei Blight Causal Agent Spores . International Journal of Innovative Approaches in Agricultural Research, 3(1), pp. 112-122.

Chicago 16th edition
Kadiri, Amina, Zineb Faiza Boukhatem, Yamina Halfaoui and Zohra Ighilhariz (2019). "Chickpea Callus Histology Inoculated with Ascochyta Rabiei Blight Causal Agent Spores ". International Journal of Innovative Approaches in Agricultural Research 3 (1):112-122. doi:10.29329/ijiaar.2019.188.11.

References

Altman, A. and P. M. Hasegawa (2011). Plant biotechnology and agriculture: prospects for the 21st century. Academic press. [Google Scholar]
Bruno, G. and L. Sparapano (2006). Effects of three esca-associated fungi on Vitis vinifera L.: I. Characterization of secondary metabolites in culture media and host responses to the pathogens in calli. Physiol. Mol. Plant Path., 69(4), 209-223. [Google Scholar]
Buchanan, B. B., W. Gruissem, K. Vickers and R. L. Jones (2015). Biochemistry and molecular biology of plants 2nd edition, John Wiley and Sons. [Google Scholar]
Clérivet, A., I. Alami, F. Breton, D. Garcia. and C. Sanier (1996). Les composés phénoliques et la résistance des plantes aux agents pathogènes. Acta Bot. Gal., 143(6), 531-538. [Google Scholar]
Dai, G. H., C. Andary, L. Mandolot-Cosson and D. Boubals (1995). Involvement of phenolic compounds in the resistance of grapevine callus to downy mildew (Plasmopara viticola). Eur. J. Plant Path., 101, 541-547. [Google Scholar]
DeZoeten, G. A., G. Gaad, G. T. Haberlac and J. P. Helgeson (1982). Infection of Tobacco callus by Phytophthora paraitca var. nicotinae. Phytopthology, 72, (7):743-746. [Google Scholar]
Doke, N. and K. Tomiyama (1980). Suppression of the hypersensitive response of potato tuber protoplasts to hyphal wall components by water soluble glucans isolated from Phytophthora infestans. Physiol. Plant Path.,16(2), 177-186. [Google Scholar]
FAO, Food and agriculture United Nations Organization (2015). (accessed 31th Nov 2015 at 5pm) http://faostat3.fao.org/ [Google Scholar]
Fondevilla, S., N. Krezdorn, B. Rotter, G. Kahl, P. Winter (2015). In planta identification of putative pathogenicity factors from the chickpea pathogen Ascochyta rabiei by de novo transcriptome sequencing using RNA-Seq and massive analysis of cDNA ends. Front. Microbiol., 6. [Google Scholar]
Fosket, D. E. and J. G. Torrey (1969). Hormonal Control of Cell Proliferation and Xylem Differentiation in Cultured Tissues of Glycine max var. biloxi. Plant Physiol., 44(6), 871-880. [Google Scholar]
Gallão, M. I., A. L. Cortelazzo, M. P. S. Fevereiro and E. S. Brito (2007). Response to chitin in suspension-cultured Citrus aurantium cells. Braz. J. Plant Physiol., 19 (1), 69-76. [Google Scholar]
Gan, Y. T., K.H. M. Siddique, W. J. MacLeod, and Jayakumar (2006). Management options for minimizing the damage by Ascochyta blight (Ascochyta rabiei) in chickpea (Cicer arietinum L.). Field Crop. Res., 97, 121–134. [Google Scholar]
Gautheret, R. J. (1959). La culture des tissus végétaux: techniques et réalisations. Masson. [Google Scholar]
Gueye, B., F. Morcillo, M. Collin, D. Gargani, P. Overvoorde, F. Aberlenc-Bertossi, J. L. Verdeil (2009). Acquisition of callogenic capacity in date palm leaf tissues in response to 2, 4-D treatment. Plant Cell Tissue Organ Cult., 99 (1), 35-45. [Google Scholar]
Gupta, S., N. Nadarajan and D. S. Gupta (2014). Legumes in the Omic Era. Springer-Verlag. [Google Scholar]
Gustine, D. L., R. T. Sherwood and C. P. Vance (1978). Regulation of Phytoalexin Synthesis in Jackbean Callus Cultures. Plant Physiol., 61, 226-230. [Google Scholar]
Höhl, B., M. Pfautsch, W. Barz (1990). Histology of disease development in resistant and susceptible cultivars of chickpea (Cicer arietinum L.) inoculated with spores of Ascochyta rabiei. J. Phytopath., 129, 31-45. [Google Scholar]
Holliday, M. and W. Klarman (1979). Expression of disease reaction types in soybean callus from resistant and susceptible plants. Phytopatholology, 69, 576-578. [Google Scholar]
Ingram, D. S. (1967). The expression of R-gene Resistance to Phytophthora infestans in Tissue Cultures of Solanum tuberosum. J. Gen. Microbiol., 49 (1), 99-108. [Google Scholar]
Ingram, D. S. and I. Joachim (1971). The growth of Peronospora farinosa f.sp. betae and sugar beet callus tissues in dual culture. J. Gen. Microbiol., 69 (2), 211-220. [Google Scholar]
Jang, J. C. and F. H. Tainter (1990). Cellular response of Pine callus to infection by Phytophtora cinnamomi. Phytopathology, 80 (12), 1347-1352. [Google Scholar]
Jayakumar, P., Y. Gan, B. Gossen, T. Warkentin and S. Banniza (2005). Ascochyta blight of chickpea: infection and host resistance mechanisms. Periodical Ascochyta blight of chickpea. Can. J. Plant Path., 27, 499–509. [Google Scholar]
Kadiri, A., Z. Ighilhariz, L. Bouabdallah and Z. Benbayer (2012). Étude histocytochimique des cals de Cicer arietinum L. confrontés au mycélium d’Ascochyta rabiei. Synthèse: Revue des Sciences et de la Technologie, 25(1), 32-40. [Google Scholar]
Kadiri, A., Z. Ighilhariz, L. Bouabdallah and Z. Benbayer (2013). Explant source, genotype and plant growth regulators effects on chickpea (Cicer arietinum L.) callus induction. Int. J. Agric. Crop Sci., 5(10), 1067-1073. [Google Scholar]
Kavousi, H. R., H. Marashi and A. Bagheri (2009). Expression of phenylpropanoid pathway genes in chickpea defence against race 3 of Ascochyta rabiei. Plant Path. J., 8(3), 127-132. [Google Scholar]
Kraft, B. and W. Barz (1985). Degradation of the isoflavone biochanin A and its glucoside conjugates by Ascochyta rabiei. Appl. Environ. Microbiol., 50 (1), 45-48. [Google Scholar]
Kumar, P., M. Sangwan and N. Mehta (2010). Callogenic response of calli from chickpea genotypes on exposure to culture filtrate of Ascochyta rabiei. J. Mycol. Plant Path., 40 (1), 22-26. [Google Scholar]
Lebeda, A. and L. Švábová (2010). In vitro screening methods for assessing plant disease resistance Pages 5-45 In: Mass Screening Techniques for Selecting Crops Resistant to Diseases. International Atomic Energy Agency publications. [Google Scholar]
Longevialle, M. (1974). Première description d'un cal se prêtant à la culture indéfinie, obtenu à partir d'une Campanulacée, Specularia speculum L. (DC). Bulletin de la Société Botanique de France, 121, (3-4), 89-96. [Google Scholar]
Macheix, J. J., A. Fleuriet and C. Jay-Allemand (2005). Les composés phénoliques des végétaux Un exemple de métabolites secondaires d’importance économique. Presses Polytechniques et Universitaires Romandes. [Google Scholar]
McComb, J. A., J. M. Hinch and A. E. Clarke (1987). Expression of field resistance in callus tissue inoculates with Phytophthora cinnamomi. Phytopathology, 77(2), 346-351. [Google Scholar]
McPhee, K. E., J. Croser, B. Sarmah, S. S. Ali, D. V. Amla, P. N. Rajesh and T. J. Higgins (2007). Development of transgenics in chickpea. Pages 458-473 in Chickpea breeding and management. CAB International, New Delhi, India. [Google Scholar]
Mehrotra, R. and A. Aggarwal (2013). Fundamentals of Plant Pathology. Tata McGraw-Hill Education. [Google Scholar]
Murashige, T. and F. Skoog (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum, 15, 443-497. [Google Scholar]
Neumann, K. H., A. Kumar and J. Imani (2009). Plant Cell and Tissue Culture- A Tool in Biotechnology. Basics and Application, Principles and Practice. Springer-Verlag. [Google Scholar]
O'Brien, T. P., N. Feder and M. E. McCully (1964). Polychromatic staining of Plant cell walls by toluidine blue O. Protoplasma, 59, 367-373. [Google Scholar]
Shah, T. M., M. Haq, B. Atta, S. Alam and H. Ali (2005). Evaluation of Cicer species for resistance to Ascochyta blight. Pak. J. Bot., 37 (2), 431-438. [Google Scholar]
Singh, M. and A. Singh (1992). Growth and sporulation of Neovossia indica in wheat callus culture. Indian Phytopath., 45(1), 113-115. [Google Scholar]
Singh, R., A. Sindhu, H. R. Singal and R. Singh (2003). Biochemical basis of resistance in chickpea (Cicer arietinum L.) against Fusarium wilt. Acta Phytopath. Hung., 38, 13-19. [Google Scholar]
Trigiano, R. N., C. G. Van Dyke., H. W. Spurr and D. J. Gray (1984). Infection and colonization of Tobacco callus by Preospora tabacina. Phytopathology, 74(3), 280-285. [Google Scholar]
Uchiyama, T., J. Sato and N. Ogasawara (1983). Lignification and qualitative changes of phenolic compounds in rice callus tissues. Agric. Biol. Chem.., 47 (1), 1-10. [Google Scholar]
Upadhyaya, G., M. B. Shivanna., H. S. Prakash and H. S. Shetty (1992). A novel approach to the establishment of dual cultures of pearl millet and Sclerospora graminicola. Plant Cell Tissue Organ Cult., 31 (3), 203-206. [Google Scholar]

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