Pen Academic Publishing   |  e-ISSN: 2602-4772

Original article | International Journal of Innovative Approaches in Agricultural Research 2018, Vol. 2(3) 226-243

Fungicide Tolerance and Effect of Environmental Conditions on Growth of Trichoderma spp. with Antagonistic Activity Against Sclerotinia sclerotiorum Causing White Mold of Common Bean (Phaseolus vulgaris)

Marie Amperes Bedıne Boat, Beatrice Iacomı, Modeste Lambert Sameza & Fabrice Fekam Boyom

pp. 226 - 243   |  DOI: https://doi.org/10.29329/ijiaar.2018.151.8   |  Manu. Number: MANU-1806-12-0003

Published online: September 26, 2018  |   Number of Views: 61  |  Number of Download: 202


Abstract

The present study was conducted to evaluate in vitro compatibility of commonly used agrochemicals as well as the effect of temperature, pH and salt on the growth of six Trichoderma spp. with antagonistic activity against S. sclerotiorum responsible for white mold of common bean. The results revealed that in dual culture, the mycelial growth inhibition of S. sclerotiorum ranged from 83.4 to 87.4 %.  The highest inhibition (87.4 %) was obtained with isolate T. erinaceum It-58, while the lowest inhibition (83.4 %) was caused by T. koningiopsis It-21.  Except T. asperellum It-13, antagonistic fungi were able to fully colonized pathogen in five days reaching class I antagonism according to Bell scale. The maximum inhibition percentage of volatile (54.07 %) and non-volatile compounds (68.89 %) on pathogen was respectively caused by T. asperellum It-13 and T. harzianum P-11. Fungicides affect the growth of Trichoderma differently. No growth was observed while testing compatibility of T. asperellum It-13 and T. erinaceum It-58 with Mancozeb as well as T. asperellum It-13 and T. afroharzianum P-8 with Methyl thiophanate illustrating the absence of compatibility. The excellent growth rate of Trichoderma was found at temperature range of 25–30˚C and pH range 4.5-5.5. Apart from T. asperellum It-13, all the isolates were able to grow at NaCl concentrations up to 1000 µM and were identified as superior salt-tolerant isolates.

Keywords: Antagonistic, S. sclerotiorum, Trichoderma, biological control, fungicide tolerance.


How to Cite this Article?

APA 6th edition
Boat, M.A.B., Iacomi, B., Sameza, M.L. & Boyom, F.F. (2018). Fungicide Tolerance and Effect of Environmental Conditions on Growth of Trichoderma spp. with Antagonistic Activity Against Sclerotinia sclerotiorum Causing White Mold of Common Bean (Phaseolus vulgaris). International Journal of Innovative Approaches in Agricultural Research, 2(3), 226-243. doi: 10.29329/ijiaar.2018.151.8

Harvard
Boat, M., Iacomi, B., Sameza, M. and Boyom, F. (2018). Fungicide Tolerance and Effect of Environmental Conditions on Growth of Trichoderma spp. with Antagonistic Activity Against Sclerotinia sclerotiorum Causing White Mold of Common Bean (Phaseolus vulgaris). International Journal of Innovative Approaches in Agricultural Research, 2(3), pp. 226-243.

Chicago 16th edition
Boat, Marie Amperes Bedine, Beatrice Iacomi, Modeste Lambert Sameza and Fabrice Fekam Boyom (2018). "Fungicide Tolerance and Effect of Environmental Conditions on Growth of Trichoderma spp. with Antagonistic Activity Against Sclerotinia sclerotiorum Causing White Mold of Common Bean (Phaseolus vulgaris)". International Journal of Innovative Approaches in Agricultural Research 2 (3):226-243. doi:10.29329/ijiaar.2018.151.8.

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