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Genetic analysis of pollen viability: an indicator of heat stress in sunflower (Helianthus annuus L.)

Khuram Razaq, Saeed Rauf, Muhammad Shahzad, Ejaz Ashraf & Fawad Shah

  |  DOI: 10.29329/ijiaar.2017.100.5

Manuscript Views: 79  |  Manuscript Download: 41


Pollen viability represents sporo-gametophytic tolerance to the heat stress. Therefore, pollen fertility index under heat stress can be exploited to differentiate resistant and susceptible genotypes.  Information relative to genetics of pollen viability is necessary to improve pollen viability under heat stress. Studies were initiated to develop and evaluate heat tolerant populations under controlled and field conditions. Result showed that pollen fertility index of genotypes was stable over the years. It showed moderate to high heritability, which was due to greater magnitude of genotypic effects in total phenotype. Studies showed that pollen fertility was primarily controlled by dominant type of genetic variability which showed that selection per se for the pollen fertility could be improved through recurrent selection. General combining analysis showed that gametophytic type of heat resistance was important in the inheritance of pollen viability. Heat resistance was dependent on the genotype of gametes as indicated from lack of relationship between mean performance of inbred line per se and their progenies for pollen viability.  

Keywords: Heat stress, reproductive fitness, pollen staining, floral head, sterility, abiotic stress, mobilization of reserve, gene action, dominance


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The determining performances of some onion (Allium cepa l.) varieties in Absheron conditions

Nesrin Huseynzade

  |  DOI: 10.29329/ijiaar.2017.100.1

Manuscript Views: 78  |  Manuscript Download: 44


The study covers the performances of onion (Allium cepa L.) varieties introducted from Turkiye. Collecting and analysing of gene pool materials focusing to onion is so important for actual seed selection and perspective forms. The main purpose of the study is collecting gene pool materials spesific of onion plant (Allium cepa L.) according to economic indicators, quality and yield traits and phenological observations over 12 different varieties (Barakat, Mor Soghan, Beyaz soghan, Tekirdagh soghan, Casta, Beta Panko, Red Amposta, Betaki, Panko, Metan, Erkenci, Valenciana) bringing from different regions of Turkey. Based on study results, there were big differences among varieties in the Azerbaijan conditions.

Keywords: Onion, varieties, biological, phenological, economy, gene pool


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Genetic Variability, Correlation and Path Coefficient Analysis in Seed Yield and Related Traits of Cowpea (Vigna Unguiculata (L.)Walp) Germplasm Accessions on High Altitude Area of Jos Plateau

Kwon-Ndung E.H. & Kwala T. D.

  |  DOI: 10.29329/ijiaar.2017.100.3

Manuscript Views: 77  |  Manuscript Download: 38


A field experiment was carried out at Dagwom farm at National Veterinary Research Institute (NVRI) Vom, in Jos South Local Government area of Plateau state Nigeria (Lat.09044N, long.08047E, altitude 1293.2 m above sea level) in 2013 and 2014 rainy seasons, to evaluate the genetic variability, correlation and path coefficient analysis in seed yield and related traits of cowpea. The treatments consisted of eighty (80) cowpea germplasm accessions obtained from International Institute for Tropical Agriculture (IITA) Ibadan Nigeria. The experiment was laid out in a Randomized Complete Block Design (RCBD) in two replicates. The Analysis of Variance (ANOVA) showed significant difference at (P<0.01) among the entries for all the yield and related traits accessed. High phenotypic coefficient of variability (PCV) compared with genotypic coefficient of variability (GCV) coupled with low Environmental coefficient of variability (ECV) was observed for all the yield and related traits accessed. High broad sense heritability (H2B) and genetic advance (GAM (%) was observed for the entire yield and related traits accessed except days to maturity which recorded high heritability with moderate genetic advance. Significant positive correlation was observed between grain yield and number of pod per plant (r=0.572**), hundred seed weight (r=0.504**), pod length (r=0.523**), number of secondary branches per plant (r=0.450**), number of seed per pod (r=0.431**), number of primary branches per plant (r=0.339**) plant height (r=0.285*), suggests that improving any of these characters will boost grain yield of cowpea. Path coefficient analysis showed that, number of pod per plant (P=0.65), hundred seed weight (P=0.54), number of seed per pod ((P=0.29) number of secondary branches per plant ((P=0.16), plant height (P=0.05)), and pod length ((P=0.04) contributed directly to grain yield. The result from this study therefore revealed that hundred seed weight, number of pod per plant, number of secondary branches per plant, number of seed per pod, pod length and plant height recorded high genetic variability, heritability and genetic advance coupled with high significant genotypic correlation with grain yield and contributed directly to grain yield. Therefore, these characters should be considered when planning hybridization programme for cowpea yield improvement.

Keywords: Variability, cowpea, correlation, yield


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RFLP- Based Gel Electrophoregram of DNA Primers for Acha (Fonio) Characterisation From Nigeria

Nyam D.D., Kwon-Ndung E.H. & Wuyep A. P.

  |  DOI: 10.29329/ijiaar.2017.100.6

Manuscript Views: 44  |  Manuscript Download: 31


The evaluation of the molecular diversity of Acha (Digitaria sp.) was carried out using Microsatelite primer combinations specially designed forDigitaria exilis. The amplifiedmicrosatellite fragments were also used in the other species of Digitaria namely; Digitaria barbinodis and Digitaria iburua.DNA extraction was carried and Agarose Gel Electrophoresis was conducted on the restricted amplified DNA extracts using microsatellite primers developed for Digitaria exilis. RFLPs clearly suggest a distinct separation of the three species of Acha at the molecular level (D. iburua, D. exilis and D. barbinodis) demonstrating the extent of their genetic differences at the DNA level.

Keywords: Gel, electrophoresis, RFLP, Acha


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Characterization and evaluation data of some leguminous plants stored in National Genebank

Mirzaliyeva I.A., Asadova A. & Akparov Z.I.

  |  DOI: 10.29329/ijiaar.2017.100.4

Manuscript Views: 44  |  Manuscript Download: 27


Characterization of plant genetic resources is the major description of germplazm for breeding. Their morpho-agronomic, biochemistry and molecular evoluation also plays an important role for creation new valuable varieties. With the support of information technologies it can be more effective collection, conservation and use of characterization and evluation data as a database format. So, for this purpose there were created characterization databases on leguminous plants within the structure of Central Databases System. As we mentioned above characterizing of each sample with more traits is very important for the breeding issues. We have tried to achieve it in our research also. For characterization and evaluation data we used international plant descriptors prepared by ICARDA (International Center of Agriculture in Dry Areas), FAO and Bioversity International .

It was created the leguminous plants (bean, cow pea, chickpea, horse bean, grass pea) databases by proper database management systems within the structure of Central Database (CDB) on PGR of Azerbaijan based on MS FoxPro. Evaluated samples were taken from the National Genebank collection by proper crop groups.

Keywords: germplasm, characterization, evaluation, legumineus plants, database


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Assessment of Nitrogen and Phosphorous Applications for Yield Potential of Guar Strains Under Irrigated Conditions

Muhammad Azeem Ur Rahman Khalid Khalid

  |  DOI: 10.29329/ijiaar.2017.100.2

Manuscript Views: 36  |  Manuscript Download: 19


The productive efficiency of two guar varieties BR-2017 and BR-99 was studied with variable nitrogen and phosphorous levels including 0-0, 0-60, 15-60, 30-0, 30-30, 30-60, 30-90 and 45-60 NP kg ha-1during two sowing seasons 2015 and 2016 at Agriculture Research Station, Bahawalpur. The results showed that BR-2017 performed better than the BR-99. Variable levels of nitrogen and phosphorous also enhanced the yield of guar. The results indicated that maximum average grain yield of guar 2544 kg ha-1 was recorded in BR-2017  with NP dosage of 30:60 kg ha-1 nitrogen and phosphorous that was non-significantly different (2457.5 kg ha-1) from  NP level of 30:90 kg ha-1 recorded from same variety. Results indicated that ‘BR-2017’ was more receptive to fertilizer application than the BR-99. 

Keywords: Cluster Bean, Nitrogen, Phosphorous, Varieties, Split Plot Design, ANOVA, LSD.


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